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The Secretion of Human Serum Albumin from the Yeast Saccharomyces cerevisiae Using Five Different Leader Sequences

Bio/Technology volume 8, pages 42–46 (1990)Cite this article

Abstract

We demonstrate the secretion of human serum albumin into the culture supernatant from the yeast Saccharomyces cerevisiae. Studies with five KEX2 processed leader sequences, namely the S. cerevisiae α factor, the natural human serum albumin, the Kluyveromyces lactis killer, a natural human serum albumin/α factor fusion, and a Kluyveromyces lactis killer/α factor fusion leader, are described. We show that the leader sequence used to direct secretion influences the quantity and quality of the secreted product. In designing secretion systems for heterologous proteins, one aims to maximise both the yield and fidelity of the product. Our results indicate that the choice of leader sequence and its relationship to the structural protein under study are crucial to the success of this process.

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Authors and Affiliations

  1. Delta Biotechnology Limited, Castle Court, 59 Castle Boulevard, Nottingham, NG7 1FD, Great Britain

    D. Sleep, G. P. Belfield & A. R. Goodey

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  1. D. Sleep
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  2. G. P. Belfield
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  3. A. R. Goodey
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Sleep, D., Belfield, G. & Goodey, A. The Secretion of Human Serum Albumin from the Yeast Saccharomyces cerevisiae Using Five Different Leader Sequences. Nat Biotechnol 8, 42–46 (1990). https://doi.org/10.1038/nbt0190-42

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