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Production of Recombinant Human Erythropoietin in Mammalian Cells: Host–Cell Dependency of the Biological Activity of the Cloned Glycoprotein

Bio/Technology volume 6, pages 67–71 (1988)Cite this article

Abstract

Erythropoietin (EPO), a heavily glycosylated protein, is a major stimulatory factor in erythropoiesis. The human EPO gene was engineered for expression in animal cells. Recombinant EPOs produced in two kinds of cells were isolated and their properties compared with those of human urinary EPO. The results indicated that the carbohydrates attached to the EPO peptide are responsible for the different biological activities of these proteins. The biological activities of recombinant glycoproteins produced in heterologous systems may vary depending on the host cells in which the proteins are modified.

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Author information

Author notes

  1. Noriko Takahashi: Department of Biochemistry, Nagoya City University Medical School, Mizuho-ku, Nagoya 467.

  2. Akimine Ishimoto: Department of Viral Oncology, Laboratory of Gene Analysis of Oncogenesis, Institute for Virus Research, Kyoto University, Kyoto 606.

  3. Hideo Chiba and Ryuzo Sasaki: Department of Food Science and Technology, Faculty of Agriculture, Kyoto University, Kyoto 606, Japan.

  4. Ryuzo Sasaki: Corresponding author.

Authors and Affiliations

  1. Research Institute of Life Science, Snow Brand Milk Products Co., Ltd., Tochigi

    Masaaki Goto, Kunihisa Akai, Akihiko Murakami, Chika Hashimoto, Eisuke Tsuda, Masatsugu Ueda, Gosei Kawanishi & Hideo Chiba

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  1. Masaaki Goto
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  2. Kunihisa Akai
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Goto, M., Akai, K., Murakami, A. et al. Production of Recombinant Human Erythropoietin in Mammalian Cells: Host–Cell Dependency of the Biological Activity of the Cloned Glycoprotein. Nat Biotechnol 6, 67–71 (1988). https://doi.org/10.1038/nbt0188-67

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  • DOI: https://doi.org/10.1038/nbt0188-67

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