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Ceramic Matrix for Large Scale Animal Cell Culture


A ceramic matrix having a high degree of surface area per unit volume is shown to have significant utility in the large scale culture of animal cells. The surfaces of the ceramic provide for the adhesion and growth of a wide variety of cells to densities equal to or greater than obtained with other methods such as roller bottles or microcarriers. Utilizing an automated system controlling pH and dissolved oxygen, scale-up from 0.9 m2 to 18.5 m2 of surface area was accomplished with no losses in efficiency of surface utilization. The density of Vero cells after 7–8 days culture under standard conditions averaged 6.6 × 105 cells/cm2 for each size of ceramic. Methods providing continuous monitoring of the culture through analysis of the cellular oxygen consumption rate and complete cell harvesting are described.

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  1. 1

    Van Wezel, A.L. and van der Velden-de Groot, C.A.M. 1978. Large scale cultivation of animal cells in microcarrier culture. Process Biochemistry, March:6–8.

  2. 2

    Van Wezel, A.L., Van Steenis, G., Hannik, C.A. and Cohen, H. 1978. New approach to the production of concentrated and purified inactivated polio and rabies tissue culture vaccines. Dev. Biol. Stand. 41: 159–168.

  3. 3

    Spier, R.E. 1980. Recent developments in the large scale cultivation of animal cells in monolayers. Advances in Biochemical Engineering, 14: 119–162.

  4. 4

    Tolbert, W.R. and Feder, J. 1983. Large scale cell culture technology. Annual Reports on Fermentation Processes, 6: 35–74.

  5. 5

    Lynn, J.D. and Acton, R.T. 1974. Design of a large-scale mammalian cell, suspension culture facility. Biotechnol. Bioeng. 17: 659–673.

  6. 6

    Zwerner, R.K., Cox, R.M., Lynn, J.D. and Acton, R.T. 1981. Five year perspective of the large-scale growth of mammalian cells in suspension culture. Biotechnol. Bioeng. 23: 2717–2735.

  7. 7

    Knazek, R.A., Guillino, P.M., Kohler, P.O. and Dedrick, R.L. 1972. Cell culture on artificial capillaries: an approach to tissue growth in vitro. Science 178: 65–66.

  8. 8

    Ku, K., Kuo, M.J., Delente, J., Wilde, B.S. and Feder, J. 1981. Development of a hollow-fibre system for large-scale culture of mammalian cells. Biotechnol. Bioeng. 23: 79–95.

  9. 9

    Jarvis, A.P. and Grdina, T.A. 1983. Production of biologicals from microencapsulated living cells. Biotechniques 1: 22–27.

  10. 10

    Griffiths, J.B., Thornton, B. and McEntee, I. 1982. The development and use of microcarrier and glass sphere culture techniques for the production of Herpes Simplex virus. Develop. Biol. Standard 50: 103–110.

  11. 11

    Clark, J., Hirtenstein and Gebbee, C. 1980. Critical parameters in the microcarrier culture of animal cells. Develop. Biol. Standard 46: 117–124.

  12. 12

    Tolbert, W.R., Feder, J. and Kimes, R. 1981. Large-scale rotating filter perfusion system for high-density growth of mammalian suspension cultures. In Vitro 17: 885–890.

  13. 13

    Fleischaker, R.J. and Sinskey, A.J. 1981. Oxygen demand and supply in cell culture. European J. Appl. Microbiol. Biotechnol. 12: 193–197.

  14. 14

    Erlinger, S. and Saier, M.H. 1982. Decrease in protein content and cell volume of cultured dog kidney epithelial cells during growth. In Vitro 18: 196–202.

  15. 15

    Lowry, O., Rosebrough, N., Farr, A. and Randall, R. 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193: 265–275.

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