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Conservation of T-DNA in Plants Regenerated from Hairy Root Cultures

Bio/Technology volume 2, pages 73–76 (1984)Cite this article

Abstract

T1-plants (first generation of transformed plants) regenerated from hairy root culture lines synthesized the same opines and carried T-DNA with the same restriction pattern as the parental line. Opine synthesis segregated in the progeny of T1-plants (T2-plants), Two opine-positive T2-plants had essentially the same T-DNA structure as the parental T1-plant, Clonal constitution of hairy root cultures, facility of regeneration, stability of opine synthesis, and T-DNA structure appear to be valuable attributes of the hairy root system for studies on genetic engineering of plants.

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Author notes

  1. Mary-Dell Chilton: Agricultural Biotechnology, CIBA-Geigy Corp., P.O. Box 12257, Research Triangle Park, N.C. 27709.

Authors and Affiliations

  1. GRIMP—Département de Génétique et d'Améliorations des Plantes, INRA Institut de Microbiologie, Bâtiment 409, Université de Paris-Sud, 91405, Orsay, France

    Chantal David, Mary-Dell Chilton & Jacques Tempé

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  1. Chantal David
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  2. Mary-Dell Chilton
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  3. Jacques Tempé
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David, C., Chilton, MD. & Tempé, J. Conservation of T-DNA in Plants Regenerated from Hairy Root Cultures. Nat Biotechnol 2, 73–76 (1984). https://doi.org/10.1038/nbt0184-73

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