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Crossing the Rubicon

A viral vector injected intravenously in mice crosses the blood-brain barrier and transduces widespread regions of the brain and spinal cord.

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Figure 1: Developmental changes in the structure of the blood–brain barrier and the brain extracellular matrix likely influence the capacity of AAV9 to transduce different types of brain cells.

Kim Caesar


  1. Foust, K.D. et al. Nat. Biotechnol. 27, 59–65 (2008).

    Article  Google Scholar 

  2. Lowenstein, P.R. Trends Immunol. 23, 23–30 (2002).

    Article  CAS  Google Scholar 

  3. Bechmann, I. et al. Trends Immunol. 28, 5–11 (2007).

    Article  CAS  Google Scholar 

  4. Enquist, L.W. J. Infect. Dis. 186 Suppl 2, S209–14 (2002).

    Article  Google Scholar 

  5. Glorioso, J.C. & Fink, D.J. Mol. Ther. advance online publication, doi:10.1038/mt.2008.213 (7 October 2008).

  6. Fiandaca, M.S. et al. Neurotherapeutics 5, 123–127 (2008).

    Article  Google Scholar 

  7. Cearley, C.N. & Wolfe, J.H. J. Neurosci. 27, 9928–9940 (2007).

    Article  CAS  Google Scholar 

  8. Fu, H. et al. Mol. Ther. 8, 911–917 (2003).

    Article  CAS  Google Scholar 

  9. Di Pasquale, G. & Chiorini, J.A. Mol. Ther. 13, 506–516 (2006).

    Article  CAS  Google Scholar 

  10. Donsante, A. et al. Science 317, 477 (2007).

    Article  CAS  Google Scholar 

  11. Manno, C.S. et al. Nat. Med. 12, 342–347 (2006).

    Article  CAS  Google Scholar 

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Lowenstein, P. Crossing the Rubicon. Nat Biotechnol 27, 42–44 (2009).

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