Brief Communication | Published:

Highly efficient RNA-guided base editing in mouse embryos

Nature Biotechnology volume 35, pages 435437 (2017) | Download Citation

Abstract

Base editors (BEs) composed of a cytidine deaminase fused to CRISPR–Cas9 convert cytidine to uridine, leading to single-base-pair substitutions in eukaryotic cells. We delivered BE mRNA or ribonucleoproteins targeting the Dmd or Tyr gene via electroporation or microinjection into mouse zygotes. F0 mice showed nonsense mutations with an efficiency of 44–57% and allelic frequencies of up to 100%, demonstrating an efficient method to generate mice with targeted point mutations.

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Acknowledgements

This work was supported by the Institute for Basic Science (IBS-R021-D1 to J.-S.K.).

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Author notes

    • Kyoungmi Kim
    •  & Seuk-Min Ryu

    These authors contributed equally to this work.

Affiliations

  1. Center for Genome Engineering, Institute for Basic Science, Seoul, Republic of Korea.

    • Kyoungmi Kim
    • , Seuk-Min Ryu
    • , Sang-Tae Kim
    • , Gayoung Baek
    • , Kayeong Lim
    • , Eugene Chung
    • , Sunghyun Kim
    •  & Jin-Soo Kim
  2. Department of Chemistry, Seoul National University, Seoul, Republic of Korea.

    • Seuk-Min Ryu
    • , Daesik Kim
    • , Kayeong Lim
    • , Eugene Chung
    • , Sunghyun Kim
    •  & Jin-Soo Kim

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Contributions

K.K., S.-M.R., and J.-S.K. designed the research. K.K., S.-M.R., S.-T.K., G.B., D.K., K.L., E.C. and S.K. performed the experiments. J.-S.K. supervised the research. All authors discussed the results and commented on the manuscript.

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Competing interests

J.-S.K. is a founder of and shareholder in ToolGen.

Corresponding author

Correspondence to Jin-Soo Kim.

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DOI

https://doi.org/10.1038/nbt.3816

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