Analysis | Published:

Reproducibility of computational workflows is automated using continuous analysis

Nature Biotechnology volume 35, pages 342346 (2017) | Download Citation


Replication, validation and extension of experiments are crucial for scientific progress. Computational experiments are scriptable and should be easy to reproduce. However, computational analyses are designed and run in a specific computing environment, which may be difficult or impossible to match using written instructions. We report the development of continuous analysis, a workflow that enables reproducible computational analyses. Continuous analysis combines Docker, a container technology akin to virtual machines, with continuous integration, a software development technique, to automatically rerun a computational analysis whenever updates or improvements are made to source code or data. This enables researchers to reproduce results without contacting the study authors. Continuous analysis allows reviewers, editors or readers to verify reproducibility without manually downloading and rerunning code and can provide an audit trail for analyses of data that cannot be shared.

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We would like to thank D. Balli (University of Pennsylvania) for providing the RNA-seq analysis design, K. Siewert (University of Pennsylvania) for providing the phylogenetic analysis design and A. Whan (Commonwealth Scientific and Industrial Research Organization) for contributing a Travis-CI implementation. We also thank M. Paul, Y. Park, G. Way, A. Campbell, J. Taroni and L. Zhou for serving as usability testers during the implementation of continuous analysis. This work was supported by the Gordon and Betty Moore Foundation under a Data Driven Discovery Investigator Award to C.S.G. (GBMF 4552). B.K.B.-J. was supported by a Commonwealth Universal Research Enhancement (CURE) Program grant from the Pennsylvania Department of Health and by US National Institutes of Health grants AI116794 and LM010098.

Author information


  1. Genomics and Computational Biology Graduate Group, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.

    • Brett K Beaulieu-Jones
  2. Department of Systems Pharmacology and Translational Therapeutics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.

    • Casey S Greene


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B.K.B.-J. and C.S.G. conceived the study and designed the solution. B.K.B.-J. implemented continuous analysis. B.K.B.-J. and C.S.G. wrote the manuscript.

Competing interests

The authors declare no competing financial interests.

Corresponding author

Correspondence to Casey S Greene.

Integrated supplementary information

Supplementary information

PDF files

  1. 1.

    Supplementary Text and Figures

    Supplementary Figures 1–6

  2. 2.

    Supplementary Data 1

    Top 104 most recent papers citing the manuscript used and the Custom CDF version used. The 104 most recent papers identified using Web of Science on November 14, 2016 that reference the BrainArray manuscript and the version of the Custom CDF that was specified.

  3. 3.

    Supplementary Data 2

    Top 116 most cited papers citing the manuscript used and the Custom CDF version used. The 116 most cited papers identified using Web of Science on November 14, 2016 that reference the BrainArray manuscript and the version of the Custom CDF that was specified.

CSV files

  1. 1.

    Supplementary Data 3

    Complete P values for Custom CDF version 18.

  2. 2.

    Supplementary Data 4

    Complete P values for Custom CDF version 19.

  3. 3.

    Supplementary Data 5

    Complete P values for Custom CDF version 20.

Zip files

  1. 1.

    Supplementary Source Code

    Continuous analysis source code. This includes template workflows for multiple distinct continuous analysis providers.

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