An atlas of targeted mass spectrometry coordinates provides a starting point for measuring the unmodified human proteome.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 12 print issues and online access
$209.00 per year
only $17.42 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
Kusebauch, U. et al. Human SRMAtlas: a resource of targeted assays to quantify the complete human proteome. Cell 166, 766–778 (2016).
Mertins, P. et al. Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55–62 (2016).
Zhang, B. et al. Proteogenomic characterization of human colon and rectal cancer. Nature 513, 382–387 (2014).
Zhang, H. et al. Integrated proteogenomic characterization of human high-grade serous ovarian cancer. Cell 166, 755–765 (2016).
Baker, M. Reproducibility crisis: blame it on the antibodies. Nature 521, 274–276 (2015).
Hoofnagle, A.N. & Wener, M.H. The fundamental flaws of immunoassays and potential solutions using tandem mass spectrometry. J. Immunol. Methods 347, 3–11 (2009).
Kennedy, J.J. et al. Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Nat. Methods 11, 149–155 (2014).
Hoofnagle, A.N. et al. Recommendations for the generation, quantification, storage, and handling of peptides used for mass spectrometry-based assays. Clin. Chem. 62, 48–69 (2016).
Carr, S.A. et al. Targeted peptide measurements in biology and medicine: best practices for mass spectrometry-based assay development using a fit-for-purpose approach. Mol. Cell Proteomics 13, 907–917 (2014).
Grant, R.P. & Hoofnagle, A.N. From lost in translation to paradise found: enabling protein biomarker method transfer by mass spectrometry. Clin. Chem. 60, 941–944 (2014).
Whiteaker, J.R. et al. CPTAC Assay Portal: a repository of targeted proteomic assays. Nat. Methods 11, 703–704 (2014).
Sharma, V. et al. Panorama: a targeted proteomics knowledge base. J. Proteome Res. 13, 4205–4210 (2014).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Competing interests
The authors declare no competing financial interests.
Rights and permissions
About this article
Cite this article
Paulovich, A., Whiteaker, J. Quantifying the human proteome. Nat Biotechnol 34, 1033–1034 (2016). https://doi.org/10.1038/nbt.3695
Published:
Issue Date:
DOI: https://doi.org/10.1038/nbt.3695