Papers from the literature selected by the Nature Biotechnology editors. (Follow us on Twitter, @NatureBiotech #nbtHighlight)

Massively parallel single-amino-acid mutagenesis

Kitzman, J.O. et al. Nat. Methods doi:10.1038/nmeth.3223 (5 January 2015)

The human splicing code reveals new insights into the genetic determinants of disease

Xiong, H.Y. et al. Science 347, 1254806 (2015)

Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex

Konermann, S. et al. Nature doi:10.1038/nature14136 (10 December 2014)

Engineering complex synthetic transcriptional programs with CRISPR RNA scaffolds

Zalatan, J.Z. et al. Cell doi:10.1016/j.cell.2014.11.052 (18 December 2014)

A new antibiotic kills pathogens without detectable resistance

Ling, L.L. et al. Nature doi:10.1038/nature14098 (7 January 2015)

Genetic diagnosis of developmental disorders in the DDD study: a scalable analysis of genome-wide research data

Wright, C.F. et al. Lancet doi:10.1016/S0140-6736(14)61705-0 (17 December 2014)