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How deep is enough in single-cell RNA-seq?

Guidelines for determining sequencing depth facilitate transcriptome profiling of single cells in heterogeneous populations.

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Figure 1: The effect of sequencing depth on cell identification.

Marina Corral Spence/Nature Publishing Group

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Correspondence to Yanyi Huang.

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Streets, A., Huang, Y. How deep is enough in single-cell RNA-seq?. Nat Biotechnol 32, 1005–1006 (2014). https://doi.org/10.1038/nbt.3039

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