Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Bispecific antibodies with native chain structure

Two new methods overcome a major hurdle that blocked easy and efficient design and expression of human bispecific antibodies.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

Figure 1: Expression of human bispecific antibodies.

References

  1. 1

    Kontermann, R. MAbs 4, 182–197 (2012).

    PubMed  PubMed Central  Article  Google Scholar 

  2. 2

    Spiess, C. et al. Nat. Biotechnol. 31, 753–758 (2013).

    CAS  PubMed  Article  Google Scholar 

  3. 3

    Lewis, S.M. et al. Nat. Biotechnol. 32, 191–198 (2014).

    CAS  PubMed  Article  Google Scholar 

  4. 4

    Ridgway, J.B., Presta, L.G. & Carter, P. Protein Eng. 9, 617–621 (1996).

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  5. 5

    Gunasekaran, K. et al. J. Biol. Chem. 285, 19637–19646 (2010).

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  6. 6

    Merchant, A.M. et al. Nat. Biotechnol. 16, 677–681 (1998).

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  7. 7

    Rouet, R., Lowe, D. & Christ, D. FEBS Lett. 588, 269–277 (2014).

    CAS  PubMed  Article  Google Scholar 

  8. 8

    Ewert, S., Huber, T., Honegger, A. & Pluckthun, A. J. Mol. Biol. 325, 531–553 (2003).

    CAS  PubMed  Article  Google Scholar 

  9. 9

    Dudgeon, K. et al. Proc. Natl. Acad. Sci. USA 109, 10879–10884 (2012).

    CAS  PubMed  Article  Google Scholar 

Download references

Acknowledgements

The authors thank Brendan Roome for assistance with graphics design and the generation of Figure 1.

Author information

Affiliations

Authors

Corresponding author

Correspondence to Daniel Christ.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Rouet, R., Christ, D. Bispecific antibodies with native chain structure. Nat Biotechnol 32, 136–137 (2014). https://doi.org/10.1038/nbt.2812

Download citation

Further reading

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing