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Bispecific antibodies with native chain structure

Nature Biotechnology volume 32, pages 136–137 (2014)Cite this article

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Two new methods overcome a major hurdle that blocked easy and efficient design and expression of human bispecific antibodies.

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Figure 1: Expression of human bispecific antibodies.

References

  1. Kontermann, R. MAbs 4, 182–197 (2012).

    Article  PubMed  PubMed Central  Google Scholar 

  2. Spiess, C. et al. Nat. Biotechnol. 31, 753–758 (2013).

    Article  CAS  PubMed  Google Scholar 

  3. Lewis, S.M. et al. Nat. Biotechnol. 32, 191–198 (2014).

    Article  CAS  PubMed  Google Scholar 

  4. Ridgway, J.B., Presta, L.G. & Carter, P. Protein Eng. 9, 617–621 (1996).

    Article  CAS  PubMed  Google Scholar 

  5. Gunasekaran, K. et al. J. Biol. Chem. 285, 19637–19646 (2010).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Merchant, A.M. et al. Nat. Biotechnol. 16, 677–681 (1998).

    Article  CAS  PubMed  Google Scholar 

  7. Rouet, R., Lowe, D. & Christ, D. FEBS Lett. 588, 269–277 (2014).

    Article  CAS  PubMed  Google Scholar 

  8. Ewert, S., Huber, T., Honegger, A. & Pluckthun, A. J. Mol. Biol. 325, 531–553 (2003).

    Article  CAS  PubMed  Google Scholar 

  9. Dudgeon, K. et al. Proc. Natl. Acad. Sci. USA 109, 10879–10884 (2012).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Acknowledgements

The authors thank Brendan Roome for assistance with graphics design and the generation of Figure 1.

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Authors and Affiliations

  1. Romain Rouet & Daniel Christ are at the Garvan Institute of Medical Research and at the University of New South Wales, Faculty of Medicine, Darlinghurst, Sydney, Australia.,

    Romain Rouet & Daniel Christ

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  1. Romain Rouet
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  2. Daniel Christ
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Correspondence to Daniel Christ.

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The authors declare no competing financial interests.

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Rouet, R., Christ, D. Bispecific antibodies with native chain structure. Nat Biotechnol 32, 136–137 (2014). https://doi.org/10.1038/nbt.2812

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