We employ the CRISPR-Cas system of Streptococcus pyogenes as programmable RNA-guided endonucleases (RGENs) to cleave DNA in a targeted manner for genome editing in human cells. We show that complexes of the Cas9 protein and artificial chimeric RNAs efficiently cleave two genomic sites and induce indels with frequencies of up to 33%.
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This work was supported by the National Research Foundation of Korea (2012-0001225) and ToolGen, Inc. We thank Jae Kyung Chon for bioinformatic analysis.
S.W.C., S.K. and J.-S.K. have filed a patent based on this work.
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Cho, S., Kim, S., Kim, J. et al. Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease. Nat Biotechnol 31, 230–232 (2013). https://doi.org/10.1038/nbt.2507
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