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Direct cloning of large genomic sequences

Nature Biotechnology volume 30, pages 405–406 (2012)Cite this article

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The discovery of an efficient mechanism of homologous recombination between two linear DNA substrates provides a new method for direct cloning.

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Figure 1: Strategies for heterologous expression of a gene cluster from genomic DNA.

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Authors and Affiliations

  1. Ryan E. Cobb is in the Department of Chemical and Biomolecular Engineering at the University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.,

    Ryan E Cobb

  2. Huimin Zhao is in the Departments of Chemical and Biomolecular Engineering, Chemistry, Biochemistry and Bioengineering, and the Institute for Genomic Biology at the University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.,

    Huimin Zhao

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  1. Ryan E Cobb
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  2. Huimin Zhao
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Correspondence to Huimin Zhao.

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The authors declare no competing financial interests.

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Cobb, R., Zhao, H. Direct cloning of large genomic sequences. Nat Biotechnol 30, 405–406 (2012). https://doi.org/10.1038/nbt.2207

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