Using a flow cytometry–based screen of commercial antibodies, we have identified cell-surface markers for the separation of pancreatic cell types derived from human embryonic stem (hES) cells. We show enrichment of pancreatic endoderm cells using CD142 and of endocrine cells using CD200 and CD318. After transplantation into mice, enriched pancreatic endoderm cells give rise to all the pancreatic lineages, including functional insulin-producing cells, demonstrating that they are pancreatic progenitors. In contrast, implanted, enriched polyhormonal endocrine cells principally give rise to glucagon cells. These antibodies will aid investigations that use pancreatic cells generated from pluripotent stem cells to study diabetes and pancreas biology.
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We thank O. Madsen (Hagedorn Research Institute), C. Wright (Vanderbilt University), J. Johnson (UT Southwestern Medical Center) and BD Biosciences for providing antibodies and A. Elefanty and E. Stanley (Monash University) for providing the MEL1 hES cell line. The CyT203 and CyT49 hES cell lines were derived with partial funding from the Juvenile Diabetes Research Foundation.
The authors are employees or former employees of ViaCyte (formerly Novocell).
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Kelly, O., Chan, M., Martinson, L. et al. Cell-surface markers for the isolation of pancreatic cell types derived from human embryonic stem cells. Nat Biotechnol 29, 750–756 (2011). https://doi.org/10.1038/nbt.1931
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