Abstract
The cullin–RING ubiquitin E3 ligase (CRL) family comprises over 200 members in humans. The COP9 signalosome complex (CSN) regulates CRLs by removing their ubiquitin-like activator NEDD8. The CUL4A–RBX1–DDB1–DDB2 complex (CRL4ADDB2) monitors the genome for ultraviolet-light-induced DNA damage. CRL4ADBB2 is inactive in the absence of damaged DNA and requires CSN to regulate the repair process. The structural basis of CSN binding to CRL4ADDB2 and the principles of CSN activation are poorly understood. Here we present cryo-electron microscopy structures for CSN in complex with neddylated CRL4A ligases to 6.4 Å resolution. The CSN conformers defined by cryo-electron microscopy and a novel apo-CSN crystal structure indicate an induced-fit mechanism that drives CSN activation by neddylated CRLs. We find that CSN and a substrate cannot bind simultaneously to CRL4A, favouring a deneddylated, inactive state for substrate-free CRL4 complexes. These architectural and regulatory principles appear conserved across CRL families, allowing global regulation by CSN.
This is a preview of subscription content, access via your institution
Relevant articles
Open Access articles citing this article.
-
Cryo-EM structures of Gid12-bound GID E3 reveal steric blockade as a mechanism inhibiting substrate ubiquitylation
Nature Communications Open Access 01 June 2022
-
Desmosomes: emerging pathways and non-canonical functions in cardiac arrhythmias and disease
Biophysical Reviews Open Access 04 September 2021
-
Expression and purification of functional recombinant CUL2•RBX1 from E. coli
Scientific Reports Open Access 27 May 2021
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Rent or buy this article
Get just this article for as long as you need it
$39.95
Prices may be subject to local taxes which are calculated during checkout
Accession codes
Primary accessions
Electron Microscopy Data Bank
Protein Data Bank
Data deposits
The cryo-EM structures are available from the Electron Microscopy Data Bank under accessions EMD-3313 (CSN–N8CRL4A at 6.7 Å), EMD-3314 (CSN–N8CRL4A at 6.4 Å), EMD-3315 (CSN–N8CRL4A at 8.8 Å) and EMD-3316 (CSN–N8CRL4ADDB2 at 8.3 Å). The negative-stain CSN–N8CRL3∆SPOP map is available under accession EMD-3317. The coordinates and structure factors for the CSN P1 crystal form have been deposited in the PDB under accession number 4WSN.
References
Marteijn, J. A., Lans, H., Vermeulen, W. & Hoeijmakers, J. H. Understanding nucleotide excision repair and its roles in cancer and ageing. Nature Rev. Mol. Cell Biol. 15, 465–481 (2014)
Ciccia, A. & Elledge, S. J. The DNA damage response: making it safe to play with knives. Mol. Cell 40, 179–204 (2010)
Wood, R. D., Mitchell, M. & Lindahl, T. Human DNA repair genes, 2005. Mutat. Res. 577, 275–283 (2005)
Sugasawa, K. et al. UV-induced ubiquitylation of XPC protein mediated by UV-DDB-ubiquitin ligase complex. Cell 121, 387–400 (2005)
Kapetanaki, M. G. et al. The DDB1–CUL4ADDB2 ubiquitin ligase is deficient in xeroderma pigmentosum group E and targets histone H2A at UV-damaged DNA sites. Proc. Natl Acad. Sci. USA 103, 2588–2593 (2006)
Fischer, E. S. et al. The molecular basis of CRL4DDB2/CSA ubiquitin ligase architecture, targeting, and activation. Cell 147, 1024–1039 (2011)
Scrima, A. et al. Detecting UV-lesions in the genome: the modular CRL4 ubiquitin ligase does it best! FEBS Lett. (2011)
Reardon, J. T. et al. Comparative analysis of binding of human damaged DNA-binding protein (XPE) and Escherichia coli damage recognition protein (UvrA) to the major ultraviolet photoproducts: T[c,s]T, T[t,s]T, T[6–4]T, and T[Dewar]T. J. Biol. Chem. 268, 21301–21308 (1993)
Wittschieben, B. Ø., Iwai, S. & Wood, R. D. DDB1–DDB2 (xeroderma pigmentosum group E) protein complex recognizes a cyclobutane pyrimidine dimer, mismatches, apurinic/apyrimidinic sites, and compound lesions in DNA. J. Biol. Chem. 280, 39982–39989 (2005)
Wang, H. et al. Histone H3 and H4 ubiquitylation by the CUL4–DDB–ROC1 ubiquitin ligase facilitates cellular response to DNA damage. Mol. Cell 22, 383–394 (2006)
Tang, J. & Chu, G. Xeroderma pigmentosum complementation group E and UV-damaged DNA-binding protein. DNA Repair (Amst.) 1, 601–616 (2002)
Chu, G. & Chang, E. Xeroderma pigmentosum group E cells lack a nuclear factor that binds to damaged DNA. Science 242, 564–567 (1988)
Groisman, R. et al. The ubiquitin ligase activity in the DDB2 and CSA complexes is differentially regulated by the COP9 signalosome in response to DNA damage. Cell 113, 357–367 (2003)
Lingaraju, G. M. et al. Crystal structure of the human COP9 signalosome. Nature 512, 161–165 (2014)
Lyapina, S. et al. Promotion of NEDD–CUL1 conjugate cleavage by COP9 signalosome. Science 292, 1382–1385 (2001)
Takedachi, A., Saijo, M. & Tanaka, K. DDB2 complex-mediated ubiquitylation around DNA damage is oppositely regulated by XPC and Ku and contributes to the recruitment of XPA. Mol. Cell. Biol. 30, 2708–2723 (2010)
Zimmerman, E. S., Schulman, B. A. & Zheng, N. Structural assembly of cullin–RING ubiquitin ligase complexes. Curr. Opin. Struct. Biol. 20, 714–721 (2010)
Soucy, T. A. T. et al. An inhibitor of NEDD8-activating enzyme as a new approach to treat cancer. Nature 458, 732–736 (2009)
Schwechheimer, C. et al. Interactions of the COP9 signalosome with the E3 ubiquitin ligase SCFTIRI in mediating auxin response. Science 292, 1379–1382 (2001)
Chen, X., Zhang, Y., Douglas, L. & Zhou, P. UV-damaged DNA-binding proteins are targets of CUL-4A-mediated ubiquitination and degradation. J. Biol. Chem. 276, 48175–48182 (2001)
Bennett, E. J., Rush, J., Gygi, S. P. & Harper, J. W. Dynamics of Cullin–RING ubiquitin ligase network revealed by systematic quantitative proteomics. Cell 143, 951–965 (2010)
Emberley, E. D., Mosadeghi, R. & Deshaies, R. J. Deconjugation of Nedd8 from Cul1 is directly regulated by Skp1-F-box and substrate, and the COP9 signalosome inhibits deneddylated SCF by a noncatalytic mechanism. J. Biol. Chem. 287, 29679–29689 (2012)
Enchev, R. I. et al. Structural basis for a reciprocal regulation between SCF and CSN. Cell Rep . 2, 616–627 (2012)
Enchev, R. I., Schreiber, A., Beuron, F. & Morris, E. P. Structural insights into the COP9 signalosome and its common architecture with the 26S proteasome lid and eIF3. Structure 18, 518–527 (2010)
Rockel, B., Schmaler, T., Huang, X. & Dubiel, W. Electron microscopy and in vitro deneddylation reveal similar architectures and biochemistry of isolated human and Flag-mouse COP9 signalosome complexes. Biochem. Biophys. Res. Commun. 450, 991–997 (2014)
Joazeiro, C. A. et al. The tyrosine kinase negative regulator c-Cbl as a RING-type, E2-dependent ubiquitin-protein ligase. Science 286, 309–312 (1999)
Waterman, H., Levkowitz, G., Alroy, I. & Yarden, Y. The RING finger of c-Cbl mediates desensitization of the epidermal growth factor receptor. J. Biol. Chem. 274, 22151–22154 (1999)
Yokouchi, M. et al. Ligand-induced ubiquitination of the epidermal growth factor receptor involves the interaction of the c-Cbl RING finger and UbcH7. J. Biol. Chem. 274, 31707–31712 (1999)
Lorick, K. L. et al. RING fingers mediate ubiquitin-conjugating enzyme (E2)-dependent ubiquitination. Proc. Natl Acad. Sci. USA 96, 11364–11369 (1999)
Freemont, P. S., Hanson, I. M. & Trowsdale, J. A novel cysteine-rich sequence motif. Cell 64, 483–484 (1991)
Zheng, N., Wang, P., Jeffrey, P. D. & Pavletich, N. P. Structure of a c-Cbl–UbcH7 complex: RING domain function in ubiquitin-protein ligases. Cell 102, 533–539 (2000)
Zheng, N. et al. Structure of the Cul1–Rbx1–Skp1–F boxSkp2 SCF ubiquitin ligase complex. Nature 416, 703–709 (2002)
Scott, D. C. et al. Structure of a RING E3 trapped in action reveals ligation mechanism for the ubiquitin-like protein NEDD8. Cell 157, 1671–1684 (2014)
Li, T., Chen, X., Garbutt, K. C., Zhou, P. & Zheng, N. Structure of DDB1 in complex with a paramyxovirus V protein: viral hijack of a propeller cluster in ubiquitin ligase. Cell 124, 105–117 (2006)
Scrima, A. et al. Structural basis of UV DNA-damage recognition by the DDB1–DDB2 complex. Cell 135, 1213–1223 (2008)
Angers, S. et al. Molecular architecture and assembly of the DDB1–CUL4A ubiquitin ligase machinery. Nature 443, 590–593 (2006)
Fischer, E. S. et al. Structure of the DDB1–CRBN E3 ubiquitin ligase in complex with thalidomide. Nature 512, 49–53 (2014)
Ito, T. et al. Identification of a primary target of thalidomide teratogenicity. Science 327, 1345–1350 (2010)
Lopez-Girona, A. et al. Cereblon is a direct protein target for immunomodulatory and antiproliferative activities of lenalidomide and pomalidomide. Leukemia 26, 2326–2335 (2012)
Krönke, J. et al. Lenalidomide causes selective degradation of IKZF1 and IKZF3 in multiple myeloma cells. Science 343, 301–305 (2014)
Lu, G. et al. The myeloma drug lenalidomide promotes the cereblon-dependent destruction of Ikaros proteins. Science 343, 305–309 (2014)
Brownell, J. E. et al. Substrate-assisted inhibition of ubiquitin-like protein-activating enzymes: the NEDD8 E1 inhibitor MLN4924 forms a NEDD8–AMP mimetic in situ. Mol. Cell 37, 102–111 (2010)
Chew, E.-H. & Hagen, T. Substrate-mediated regulation of cullin neddylation. J. Biol. Chem. 282, 17032–17040 (2007)
Pierce, N. W. et al. Cand1 promotes assembly of new SCF complexes through dynamic exchange of F box proteins. Cell 153, 206–215 (2013)
Dubiel, W. Resolving the CSN and CAND1 paradoxes. Mol. Cell 35, 547–549 (2009)
Kucukelbir, A., Sigworth, F. J. & Tagare, H. D. Quantifying the local resolution of cryo-EM density maps. Nature Methods 11, 63–65 (2014)
Landau, M. et al. ConSurf 2005: the projection of evolutionary conservation scores of residues on protein structures. Nucleic Acids Res. 33, W299–W302 (2005)
Acknowledgements
We thank K. Böhm for technical assistance in protein expression and purification, D. Hess and J. Seebacher for mass spectrometry analysis, and M. Jones, B. Martoglio, R. Assenberg, C. Logel, I. Bechtold and M. Renatus for help developing the TR-FRET and PT22 CSN assays. We thank B. Anderson and A. Fecteau-Lefebvre (‘C-CINA’) for technical support and TEM maintenance. We are grateful to T. Walz and D. Barford for help and discussions. Part of this work was performed at beamline X10SA of the Swiss Light Source. This work was supported by the Novartis Research Foundation and grants to N.H.T. from the European Research Council (ERC-2014-ADG 666068 CSNCRL) and to H.S. from the Swiss initiative for Systems Biology (SystemsX.ch grant ‘C-CINA’). G.P. was supported by long-term fellowships of the European Molecular Biology Organization (EMBO; ALTF-1350-2013) and the Human Frontier Science Program (HFSP; LT000210/2014). W.I.M. was supported by the Boehringer Ingelheim Fonds.
Author information
Authors and Affiliations
Contributions
S.C., E.S.F. and N.H.T, co-led the study. S.C. prepared the specimens for EM data collection and collected EM data with contributions from K.N.G.. S.C. performed EM data processing. E.S.F. developed the binding and activity assays with input from U.H., G.P. and A.P. G.M.L. designed and created CSN mutants with input from S.C. E.S.F, M.F., G.M.L. and W.A. purified proteins for crystallography, cryo-EM and biochemical assays. A.P. and E.S.F. performed functional assays and analysed the results. G.M.L. crystallized CSN. R.D.B. and G.M.L collected the X-ray diffraction data. R.D.B carried out the crystallographic analysis, built the cryo-EM models with input from S.C., and interpreted the results with S.C. and N.H.T.. K.N.G., R.S.P. and H.S. provided access to TEM microscopes and initial training. W.I.M. designed the RBX1–CSN4 fusion construct and purified protein for EM studies. R.E.J.B. and R.B.T. designed and performed the chemical syntheses. S.M. and K.S. performed cellular assays. S.C., E.S.F., R.D.B. and N.H.T. wrote the manuscript with input from G.P.
Corresponding author
Ethics declarations
Competing interests
The authors declare no competing financial interests.
Extended data figures and tables
Extended Data Figure 1 Atomic models of the canonical CRL families.
a, CUL1–RBX1–SKP1–SKP2 (PDB accession codes 1LDK and 1FQV). b, CUL1–RBX1–SKP1–FBW7 (PDBs accession codes 1LDK and 2OVP). c, CUL2–RBX1–ElonginB–ElonginC–VHL (PDB accession codes 1LDK and 1VCB). d, Dimeric CUL3–RBX1–SPOP. The SPOP protein includes a BTB and a MATH domain. (PDB accession codes 1LDK, 4EOZ and 3HU6). e, CUL4A–RBX1–DDB1–DDB2 (PDB accession code 4A0K). f, CUL4A–RBX1–DDB1–CRBN (PDB accession codes 4A0K and 4CI1). g, CUL4A–RBX1–DDB1–CSA (PDB accession codes 4A0K and 4A11). h, CUL5–RBX1–ElonginB–ElonginC–SOCS2 (PDB accession codes 1LDK, 4JGH and 2C9W).
Extended Data Figure 2 Classification and refinement procedures for the CSN–N8CRL4ADDB2 complex.
a, Representative micrographs (scale bar, 50 nm; the whole data set includes 1,427 micrographs) and reference-free two-dimensional (2D) class averages (scale bar, 10 nm). b, After reference-free 2D classification, the initial model obtained from preliminary cryo-EM studies of CSN–N8CRL4ADDB2 was low-pass filtered to 60 Å and subjected to 3D classification to discard large misassembled particles. Class I was subjected to a second round of 2D classification and particles showing an intact assembly were merged with the particles included in the Class III, leading to 16,090 particles. The final model was refined to 8.3 Å resolution. c, Angular distribution plot. d, Gold-standard Fourier shell correlation curve (FSC).
Extended Data Figure 3 Classification and refinement procedures for the CSN–N8CRL4A complex.
a, Representative micrographs (scale bar, 50 nm; the whole data set includes 2,626 micrographs) and reference-free 2D class averages (scale bar, 10 nm). b, After 2D classification, the initial model obtained from preliminary cryo-EM studies of CSN–N8CRL4ADDB2 was low-pass filtered to 60 Å and subjected to a first round of 3D classification to discard large misassembled particles. Particles included in class I and class III were combined and refined in a 8.8 Å reconstruction map. To improve the resolution, we performed further 3D classification into two classes. The most populated (73%, 63,269 particles) class shows better resolution and finer details as suggested by the slice-through Z of the map (black circles with with particles on the left and right side of the 3D model). Refinement with a soft mask around the more rigid part of the complex led to a 6.4 Å resolution map. Refinement of the same set of particles with a soft mask around the whole complex let to a 6.7 Å resolution map. The 6.7 Å reconstruction is coloured according to the local resolution as estimated with ResMap46. c, The same set of particles that led to the 8.8 Å structure were also subjected to particle polishing and 3D focus classification on CSN (mask drawn as a blue line) with signal subtraction of CUL4A–DDB1. The data was divided into four classes revealing the dynamic of the CSN5–CSN6 heterodimer on cullin binding (Fig. 3a–c) (highlighted with a dashed red circle). Refinement of class I and class III led to a 7.8 Å and 8.8 Å resolution maps, respectively. d, Angular distribution of the particles included in the 6.4 Å (and 6.7 Å) data set. e, Gold-standard Fourier shell correlation curves (FSC) for the four refined models.
Extended Data Figure 4 The overall fold of each protein within the CSN–N8CRL4A complex.
a–r, Unless specified, the individual subunits found in 6.4 Å CSN–N8CRL4A map are shown as cartoon representation. The cryo-EM density of each subunit is shown as a coloured mesh. CSN1 (a); CSN2 (b); CSN3 (c); CSN4 (d) CSN5 (8.8 Å resolution CSN–N8CRL4A map) (e) and CSN6 (8.8 Å resolution CSN–N8CRL4A map) (f); CSN7 (g); and CSN8 (h). Cryo-EM density and architecture of the CSN helical bundle found in the 6.7 Å CSN–N8CRL4A cryo-EM map (i); C-terminal (j) and N-terminal domain of CUL4A (k); RBX1 (l); DDB1 WD40 β-propeller domains BPA (m); BPB (n); and BPC (o); NEDD8 (N8) and WHB binding to CSN5 as shown in the 8.8 Å resolution CSN–N8CRL4A map (Fig. 1d) (p).
Extended Data Figure 5 Biophysical characterization of CSN mutants.
Determination of steady-state kinetics using the N8–PT22CRL4ADDB2 substrate. Initial rates observed following incubation of CSN or mutants (at 10 nM, unless specified otherwise) with increasing concentrations of N8–PT22CRL4ADDB2. The fit of the observed data to the Michaelis–Menten equation is shown in a, for 2 nM wild-type CSN (the experiment shown is a representative of four technical replicates (n = 2)); b, 20 nM CSN1279–527 (n = 3); c, 10 nM CSN4180–406 (n = 3). d, Dose response curve for the CSN–N8-Alexa488CRL4ADDB2 complex obtained by increasing concentration of unlabelled N8CRL4A (n = 3). e, As in d, but with increasing concentration of CRL4ADDB2 (n = 2). f, As in d, but with increasing concentration of unlabelled CSN (n = 2). g, Biotinylated CSN active site mutant (CSNASM) at 10 nM and terbium–streptavidin (Tb–streptavidin) conjugate (4 nM) were mixed with increasing concentrations of N8–Alexa488CRL4ADDB2 and the dissociation constants of CSN for N8–Alexa488CRL4ADDB2 obtained by fitting the TR-FRET signal assuming equimolar binding (see Supplementary Information). Three technical replicates are shown as overlaid curves to illustrate the reproducibility of the experiment. h, Binding of CSNASM (10 nM) and biotin-labelled ASM variants of CSN mutants at 10 nM (CSN (CSN2 (E33K, Y36A, K40D))), (CSN (CSN2 (R105E, K143D, R146E, K150D))), and (CSN (CSN2 (D266R, E267R))) to N8–Alexa488CRL4ADDB2 (n = 2). i, Steady-state kinetics for the CSN mutants (2 nM) used in h (n = 2). j, k, l, Binding of N8-Alexa488CRL4ADDB2 to CSNASM (10 nM) and biotin-labelled ASM variants of the CSN mutants at 10 nM (CSN (CSN2 (F259A, S296A, Q297A, E298K, K300D))) and (CSN (CSN4 (E165K, N169A, R170E))) (j) (n = 2); (CSN (CSN1 (R225E, H253A, Y257A, K260E))) (k) (n = 2); (CSN (CSN2 (D182K, D183K, E185K, D186R, D187K, K189D, K190E))) (l) (n = 2). m, n Steady-state kinetics for the CSN mutants (CSN (CSN71–218)) (2 nM) and (CSN (CSN730–218)) (2 nM) (m) (n = 2); 150 nM ((CSN(CSN6192-327)) (n = 2) and 2 nM (CSN (CSN1 (R225E, H253A, Y257A, K260E))) (n = 2) (n). In n, the deviation between fit and data for (CSN (CSN6192-327)) may suggest cooperative behaviour. o, Binding of the biotin-labelled ASM variant of the CSN mutant at 10 nM (CSN (CSN152–507, CSN31–400, CSN5 (S284A, F285A, L287A, H292A, R294D, K295D), CSN61–313)) to N8–Alexa488CRL4ADDB2 (n = 2). Data are technical replicates as indicated and shown as individual data points (for n ≤ 2) or mean ± s.d. (for n ≥ 3) in addition to the fit to the mean curve.
Extended Data Figure 6 Structural plasticity of CSN–CRL4 complexes.
a, The CUL4A C-terminal domain (CUL4ACTD) is coloured by conservation across all cullin families (automatically defined in ConSurf)47. Residues in proximity of the CSN2–CUL4ACTD interaction patches (shown as spheres) (Fig. 2a) are highly conserved. b, Superimposing WHA, α/β-domain and 4-HB domains (shown as transparent cartoon) of different structures (PDB accession codes 2HYE, 4A0K, 4P5O, 3DQV) reveal flexibility of the WHB domain. c, Superimposition of the CAND1–CUL4B–RBX1 structure (PDB accession code 4A0C, cyan) with the CUL4A–RBX1 conformation found in the cryo-EM CSN–N8CRL4A structures. d, The DDB1 inter-domain hinge shown in the close-up enables the DDB1 BPA and BPC subdomains to adopt different position with respect to DDB1 BPB allowing the CUL4A arm to swing at least 150°. The presence or absence of the substrate receptors does not affect the position of the cullin arm within CSN–N8CRL4A complexes. e, Rigid body dissection of apo-CSN. Boundaries (1) CSN1: 77–463; (2) CSN2: 30–180; (3) CSN2: 192–411; (4) CSN3: 3–345; (5) CSN4: 1–179; (6) CSN4: 180–298 (7) CSN4: 266–366; (8) CSN5: 24–249, CSN6: 29–296; (9) CSN7: 8–165; (10) CSN8: 11–167 (11) helical bundle: CSN1: 464–505, CSN2: 412–443, CSN3: 346–403, CSN4: 367–406, CSN5: 250–333, CSN6: 217–316, CSN7: 166–216, CSN8: 194–209. f, Structural comparison across 10 CSN crystallographic conformers, including P1 and P31 (PDB accession codes 4D10 and 4D18) crystals, provides evidence for large-scale conformational change. The models are coloured by levels of root mean squared deviation (RMSD). g, Superimposition of the PCI ring from apo-CSN (PDB accession code 4D18, grey) and the cullin-bound CSN conformation (Fig. 3d) (coloured as in Fig. 1). h, Superimposition of the helical bundle from apo-CSN (PDB accession code 4D18, grey) and in the cullin-bound CSN conformation showing structural rearrangement of the helical bundle on cullin binding (coloured as in Fig. 1).
Extended Data Figure 8 Substrate binding inhibits CSN activity.
StrepII-tagged CSN was immobilized on Strep-Tactin beads and incubated with either CRL4ADDB2 (a) or N8CRL4ADDB2 (b). Following excessive washing, CRL4ADDB2 complexes remained bound to immobilized CSN (lane II). The immobilized complexes were subsequently incubated with 10 μM of a 31-bp THF oligo. Coomassie stained SDS–PAGE analysis revealed that CRL4ADDB2 and N8CRL4ADDB2 were dislodged in presence of DNA (lane IV) but not in a buffer control (lane IV buffer control in b). c, Increasing amounts of CRL4ADDB2 were mixed with a 16-bp Cy5-THF oligo and the dissociation constant Kd was determined by fitting the data to a model assuming one binding site (n = 2). d, Increasing concentrations of unlabelled 21-bp 6-4PP oligo were mixed with 16-bp Cy5-THF (100 nM) and CRL4ADDB2 (500 nM). The half-maximum effective concentration (EC50) was used to calculate the Ki (see Supplementary Information) inhibition constant (Ki is equivalent to Kd of 21-bp 6-4PP for CRL4ADDB2) (n = 2). e, Dose-response experiment with increasing concentration of lenalidomide titrated into CSN (2 nM) and N8–PT22CRL4ACRBN (500 nM) (n = 1). f, Binding of thalidomide–Cy5 to DDB1–CRBN (n = 1). g, Increasing concentration of lenalidomide–NEDD8 titrated into thalidomide–Cy5 (25 nM) and DDB1–CRBN (100 nM). EC50 was used to calculate Ki. These data indicate that lenalidomide–NEDD8 directly compete with thalidomide binding to DDB1-CRBN, and hence uses the same binding site (n = 1). h, i, Affinity of the biotin-labelled ASM variant of the CSN mutant (CSN (CSN1 (R225E, H253A, Y257A, K260E))) (10 nM) for N8CRL1SKP2 (n = 2) (h) and N8CRL3∆SPOP (i), determined by mixing increasing amounts of N8-Alexa488CRL1SKP2 and N8-Alexa488CRL3∆SPOP respectively with CSN (10 nM) and Tb-streptavidin (4 nM). Data were fitted as described in the Supplementary Information (n = 2). j, Steady-state kinetics of 2 nM CSN with a N8–PT22CRL4A substrate (n = 3). k, Binding curves for the biotin-labelled ASM variant of the CSN mutants (listed below the panel) to N8–Alexa488CRL4A (n = 2). One representative fit for each CSN mutant is shown from two technical replicates. l, Steady-state kinetics of CSN mutants (CSN (CSN2 (D182K, D183K, E185K, D186R, D187K, K189D, K190E))) and (CSN (CSN2 (F259A, S296A, Q297A, E298K, K300D))) with a N8–PT22CRL4ADDB2 substrate (n = 2). m, Steady-state kinetics of 2 nM CSN mutant (CSN (CSN4 (E165K, N169A, R170E))) with a N8–PT22CRL4ADDB2 substrate (n = 2). Data are technical replicates as indicated and shown as individual data points (for n ≤ 2) or mean ± s.d. (for n ≥ 3) in addition to the fit to the mean curve.
Extended Data Figure 9 Negative-stain single-particle EM reconstructions of CSN–N8CRL4ACRBN and CSN–N8CRL3∆SPOP.
a, Representative micrographs (scale bar, 50 nm; the whole data set includes 265 micrographs) and reference-free 2D class averages (scale bar, 10 nm) for CSN–N8CRL4ACRBN. b, Gold-standard Fourier shell correlation curve (FSC) for the CSN–N8CRL4ACRBN reconstruction. c, Angular distribution for CSN–N8CRL4ACRBN. d, CSN–N8CRL4ACRBN density fitted with DDB1–CRBN (PDB accession code 4CI1) and the cryo-EM CSN–N8CRL4A model (without DDB1). e, Representative micrographs (scale bar, 50 nm; the whole data set includes 104 micrographs) and reference-free 2D class averages (scale bar, 10 nm) for CSN–N8CRL3∆SPOP. f, Gold-standard FSC for the CSN-N8CRL3∆SPOP reconstruction. g, Angular distribution of the CSN–N8CRL3∆SPOP particles. h, Crystallographic models of CUL3NTD-BTB (PDB accession code 4EOZ), the substrate receptor MATH (PDB accession code 3HQI), and the NEDD8-engaged CSN cryo-EM model fitted in the CSN–N8CRL3∆SPOP negative-stain EM map. Modelling of the N-terminal MATH domain of SPOP (residues 28–166, Extended Data Fig. 1d) reveals its position within the CSN–N8CRL3∆SPOP architecture. i, Fit of the cullin-bound CSN model (Fig. 3d) and crystallographic models of N8CRL1SKP2/CKS1/p27 (PDB accession codes 1LDK, 3DQV, 2ASS) into the CSN–N8CRL1SKP2/CKS1 negative-stain map (EMDB accession code 2173). j, Fit of the cullin-bound CSN model (Fig. 3d) and crystallographic models of N8CRL1FBW7/CycE (PDB accession codes 1LDK and 2OVP) into the CSN–N8CRL1FBW7 negative-stain map (EMDB accession code 2174).
Supplementary information
Supplementary Information
This file contains Supplementary Text and Data and additional references. (PDF 731 kb)
Rights and permissions
About this article
Cite this article
Cavadini, S., Fischer, E., Bunker, R. et al. Cullin–RING ubiquitin E3 ligase regulation by the COP9 signalosome. Nature 531, 598–603 (2016). https://doi.org/10.1038/nature17416
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/nature17416
This article is cited by
-
NUMB facilitates autophagy initiation through targeting SCFβ-TrCP2 complex
Cell Death & Differentiation (2022)
-
Cryo-EM structures of Gid12-bound GID E3 reveal steric blockade as a mechanism inhibiting substrate ubiquitylation
Nature Communications (2022)
-
Targeting neddylation E2s: a novel therapeutic strategy in cancer
Journal of Hematology & Oncology (2021)
-
Expression and purification of functional recombinant CUL2•RBX1 from E. coli
Scientific Reports (2021)
-
IP6-assisted CSN-COP1 competition regulates a CRL4-ETV5 proteolytic checkpoint to safeguard glucose-induced insulin secretion
Nature Communications (2021)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
