Coleoid cephalopods (octopus, squid and cuttlefish) are active, resourceful predators with a rich behavioural repertoire1. They have the largest nervous systems among the invertebrates2 and present other striking morphological innovations including camera-like eyes, prehensile arms, a highly derived early embryogenesis and a remarkably sophisticated adaptive colouration system1,3. To investigate the molecular bases of cephalopod brain and body innovations, we sequenced the genome and multiple transcriptomes of the California two-spot octopus, Octopus bimaculoides. We found no evidence for hypothesized whole-genome duplications in the octopus lineage4,5,6. The core developmental and neuronal gene repertoire of the octopus is broadly similar to that found across invertebrate bilaterians, except for massive expansions in two gene families previously thought to be uniquely enlarged in vertebrates: the protocadherins, which regulate neuronal development, and the C2H2 superfamily of zinc-finger transcription factors. Extensive messenger RNA editing generates transcript and protein diversity in genes involved in neural excitability, as previously described7, as well as in genes participating in a broad range of other cellular functions. We identified hundreds of cephalopod-specific genes, many of which showed elevated expression levels in such specialized structures as the skin, the suckers and the nervous system. Finally, we found evidence for large-scale genomic rearrangements that are closely associated with transposable element expansions. Our analysis suggests that substantial expansion of a handful of gene families, along with extensive remodelling of genome linkage and repetitive content, played a critical role in the evolution of cephalopod morphological innovations, including their large and complex nervous systems.
Soft-bodied cephalopods such as the octopus (Fig. 1a) show remarkable morphological departures from the basic molluscan body plan, including dexterous arms lined with hundreds of suckers that function as specialized tactile and chemosensory organs, and an elaborate chromatophore system under direct neural control that enables rapid changes in appearance1,8. The octopus nervous system is vastly modified in size and organization relative to other molluscs, comprising a circumesophageal brain, paired optic lobes and axial nerve cords in each arm2,3. Together these structures contain nearly half a billion neurons, more than six times the number in a mouse brain2,9. Extant coleoid cephalopods show extraordinarily sophisticated behaviours including complex problem solving, task-dependent conditional discrimination, observational learning and spectacular displays of camouflage1,10 (Supplementary Videos 1 and 2).
To explore the genetic features of these highly specialized animals, we sequenced the Octopus bimaculoides genome by a whole-genome shotgun approach (Supplementary Note 1) and annotated it using extensive transcriptome sequence from 12 tissues (Methods and Supplementary Note 2). The genome assembly captures more than 97% of expressed protein-coding genes and 83% of the estimated 2.7 gigabase (Gb) genome size (Methods and Supplementary Notes 1, 2, 3). The unassembled fraction is dominated by high-copy repetitive sequences (Supplementary Note 1). Nearly 45% of the assembled genome is composed of repetitive elements, with two bursts of transposon activity occurring ∼25-million and ∼56-million years ago (Mya) (Supplementary Note 4).
We predicted 33,638 protein-coding genes (Methods and Supplementary Note 4) and found alternate splicing at 2,819 loci, but no locus showed an unusually high number of splice variants (Supplementary Note 4). A-to-G discrepancies between the assembled genome and transcriptome sequences provided evidence for extensive mRNA editing by adenosine deaminases acting on RNA (ADARs). Many candidate edits are enriched in neural tissues7 and are found in a range of gene families, including ‘housekeeping’ genes such as the tubulins, which suggests that RNA edits are more widespread than previously appreciated (Extended Data Fig. 1 and Supplementary Note 5).
Based primarily on chromosome number, several researchers proposed that whole-genome duplications were important in the evolution of the cephalopod body plan4,5,6, paralleling the role ascribed to the independent whole-genome duplication events that occurred early in vertebrate evolution11. Although this is an attractive framework for both gene family expansion and increased regulatory complexity across multiple genes, we found no evidence for it. The gene family expansions present in octopus are predominantly organized in clusters along the genome, rather than distributed in doubly conserved synteny as expected for a paleopolyploid12,13 (Supplementary Note 6.2). Although genes that regulate development are often retained in multiple copies after paleopolyploidy in other lineages, they are not generally expanded in octopus relative to limpet, oyster and other invertebrate bilaterians11,14 (Table 1 and Supplementary Notes 7.4 and 8).
Hox genes are commonly retained in multiple copies following whole-genome duplication15. In O. bimaculoides, however, we found only a single Hox complement, consistent with the single set of Hox transcripts identified in the bobtail squid Euprymna scolopes with PCR16. Remarkably, octopus Hox genes are not organized into clusters as in most other bilaterian genomes15, but are completely atomized (Extended Data Fig. 2 and Supplementary Note 9). Although we cannot rule out whole-genome duplication followed by considerable gene loss, the extent of loss needed to support this claim would far exceed that which has been observed in other paleopolyploid lineages, and it is more plausible that chromosome number in coleoids increased by chromosome fragmentation.
Mechanisms other than whole-genome duplications can drive genomic novelty, including expansion of existing gene families, evolution of novel genes, modification of gene regulatory networks, and reorganization of the genome through transposon activity. Within the O. bimaculoides genome, we found evidence for all of these mechanisms, including expansions in several gene families, a suite of octopus- and cephalopod-specific genes, and extensive genome shuffling.
In gene family content, domain architecture and exon–intron structure, the octopus genome broadly resembles that of the limpet Lottia gigantea17, the polychaete annelid Capitella teleta17 and the cephalochordate Branchiostoma floridae14 (Supplementary Note 7 and Extended Data Fig. 3). Relative to these invertebrate bilaterians, we found a fairly standard set of developmentally important transcription factors and signalling pathway genes, suggesting that the evolution of the cephalopod body plan did not require extreme expansions of these ‘toolkit’ genes (Table 1 and Supplementary Note 8.2). However, statistical analysis of protein domain distributions across animal genomes did identify several notable gene family expansions in octopus, including protocadherins, C2H2 zinc-finger proteins (C2H2 ZNFs), interleukin-17-like genes (IL17-like), G-protein-coupled receptors (GPCRs), chitinases and sialins (Figs 1b, 2 and 3; Extended Data Figs 4, 5, 6 and Supplementary Notes 8 and 10).
The octopus genome encodes 168 multi-exonic protocadherin genes, nearly three-quarters of which are found in tandem clusters on the genome (Fig. 2b), a striking expansion relative to the 17–25 genes found in Lottia, Crassostrea gigas (oyster) and Capitella genomes. Protocadherins are homophilic cell adhesion molecules whose function has been primarily studied in mammals, where they are required for neuronal development and survival, as well as synaptic specificity18. Single protocadherin genes are found in the invertebrate deuterostomes Saccoglossus kowalevskii (acorn worm) and Strongylocentrotus purpuratus (sea urchin), indicating that their absence in Drosophila melanogaster and Caenorhabditis elegans is due to gene loss. Vertebrates also show a remarkable expansion of the protocadherin repertoire, which is generated by complex splicing from a clustered locus rather than tandem gene duplication (reviewed in ref. 19). Thus both octopuses and vertebrates have independently evolved a diverse array of protocadherin genes.
A search of available transcriptome data from the longfin inshore squid Doryteuthis (formerly, Loligo) pealeii20 also demonstrated an expanded number of protocadherin genes (Supplementary Note 8.3). Surprisingly, our phylogenetic analyses suggest that the squid and octopus protocadherin arrays arose independently. Unlinked octopus protocadherins appear to have expanded ∼135 Mya, after octopuses diverged from squid. In contrast, clustered octopus protocadherins are much more similar in sequence, either due to more recent duplications or gene conversion as found in clustered protocadherins in zebrafish and mammals21.
The expression of protocadherins in octopus neural tissues (Fig. 2) is consistent with a central role for these genes in establishing and maintaining cephalopod nervous system organization as they do in vertebrates. Protocadherin diversity provides a mechanism for regulating the short-range interactions needed for the assembly of local neural circuits18, which is where the greatest complexity in the cephalopod nervous system appears2. The importance of local neuropil interactions, rather than long-range connections, is probably due to the limits placed on axon density and connectivity by the absence of myelin, as thick axons are then required for rapid high-fidelity signal conduction over long distances. The sequence divergence between octopus and squid protocadherin expansions may reflect the notable differences between octopuses and decapodiforms in brain organization, which have been most clearly demonstrated for the vertical lobe, a key structure in cephalopod learning and memory circuits2,22. Finally, the independent expansions and nervous system enrichment of protocadherins in coleoid cephalopods and vertebrates offers a striking example of convergent evolution between these clades at the molecular level.
As with the protocadherins, we found multiple clusters of C2H2 ZNF transcription factor genes (Fig. 3a and Supplementary Note 8.4). The octopus genome contains nearly 1,800 multi-exonic C2H2-containing genes (Table 1), more than the 200–400 C2H2 ZNFs found in other lophotrochozoans and the 500–700 found in eutherian mammals, in which they form the second-largest gene family23. C2H2 ZNF transcription factors contain multiple C2H2 domains that, in combination, result in highly specific nucleic acid binding. The octopus C2H2 ZNFs typically contain 10–20 C2H2 domains but some have as many as 60 (Supplementary Note 8.4). The majority of the transcripts are expressed in embryonic and nervous tissues (Fig. 3b). This pattern of expression is consistent with roles for C2H2 ZNFs in cell fate determination, early development and transposon silencing, as demonstrated in genetic model systems23.
The expansion of the O. bimaculoides C2H2 ZNFs coincides with a burst of transposable element activity at ∼25 Mya (Fig. 3c). The flanking regions of these genes show a significant enrichment in a 70–90 base pair (bp) tandem repeat (31% for C2H2 genes versus 4% for all genes; Fisher’s exact test P value <1 × 10−16), which parallels the linkage of C2H2 gene expansions to β-satellite repeats in humans24. We also found an expanded C2H2 ZNF repertoire in amphioxus (Table 1), showing a similar enrichment in satellite-like repeats. These parallels suggest a common mode of expansion of a highly dynamic transcription factor family implicated in lineage-specific innovations.
To investigate further the evolution of gene families implicated in nervous system development and function, we surveyed genes associated with axon guidance (Table 1) and neurotransmission (Table 2), identifying their homologues in octopus and comparing numbers across a diverse set of animal genomes (Supplementary Notes 8, 9, 10). Several patterns emerged from this survey. The gene complements present in the model organisms D. melanogaster and C. elegans often showed striking departures from those seen in lophotrochozoans and vertebrates (Table 2 and Supplementary Note 10). For example, D. melanogaster encodes one member of the discs large (DLG) family, a key component of the postsynaptic scaffold. In contrast, mammals have four DLGs, which (along with other observations) led to suggestions that vertebrates possess uniquely complex synaptic machinery25. However, we found three DLGs in both octopus and limpet, suggesting that vertebrate and fly gene number differences are not necessarily diagnostic of exceptional vertebrate synaptic complexity (Supplementary Note 10.6).
Overall, neurotransmission gene family sizes in the octopus were very similar to those seen in other lophotrochozoans (Table 2 and Supplementary Note 10), except for a few strikingly expanded gene families such as the sialic acid vesicular transporters (sialins) (Supplementary Note 10.2). We did find variations in the sizes of neurotransmission gene families between human and lophotrochozoans (Table 2 and Supplementary Note 10), but no evidence for systematic expansion of these gene families in vertebrates relative to octopus or other lophotrochozoans. Although some gene families were larger in mammals or absent in lophotrochozoans (for example, ligand-gated 5-HT receptors), others were absent in mammals and present in invertebrates (for example, anionic glutamate and acetylcholine receptors). The complement of neurotransmission genes in octopus may be broadly typical for a lophotrochozoan, but our findings suggest it is also not obviously smaller than is found in mammals.
Among the octopus complement of ligand-gated ion channels, we identified a set of atypical nicotinic acetylcholine receptor-like genes, most of which are tandemly arrayed in clusters (Extended Data Fig. 7). These subunits lack several residues identified as necessary for the binding of acetylcholine26, so it is unlikely that they function as acetylcholine receptors. The high level of expression of these divergent subunits within the suckers raises the interesting possibility that they act as sensory receptors, as do some divergent glutamate receptors in other protostomes27. In addition, we identified 74 Aplysia-like and 11 vertebrate-like candidate chemoreceptors among the octopus GPCR superfamily of ∼330 genes (Extended Data Fig. 6).
We found, amid extensive transcription of octopus transposons, that a class of octopus-specific short interspersed nuclear element sequences (SINEs) is highly expressed in neural tissues (Supplementary Note 4 and Extended Data Fig. 8). Although the role of active transposons is unclear, elevated transposon expression in neural tissues has been suggested to serve an important function in learning and memory in mammals and flies28.
Transposable element insertions are often associated with genomic rearrangements29 and we found that the transposon-rich octopus genome displays substantial loss of ancestral bilaterian linkages that are conserved in other species (Supplementary Note 6 and Extended Data Fig. 9). Interestingly, genes that are linked in other bilaterians but not in octopus are enriched in neighbouring SINE content. SINE insertions around these genes date to the time of tandem C2H2 expansion (Extended Data Fig. 9d), pointing to a crucial period of genome evolution in octopus. Other transposons such as Mariner show no such enrichment, suggesting distinct roles for different classes of transposons in shaping genome structure (Extended Data Fig. 9c).
Transposable element activity has been implicated in the modification of gene regulation across several eukaryotic lineages29. We found that in the nervous system, the degree to which a gene’s expression is tissue-specific is positively correlated with the transposon load around that gene (r2 values ranging from 0.49 in the optic lobe to 0.81 in the subesophageal brain; Extended Data Fig. 8 and Supplementary Note 4). This correlation may reflect modulation of gene expression by transposon-derived enhancers or a greater tolerance for transposon insertion near genes with less complex patterns of tissue-specific gene regulation.
Using a relaxed molecular clock, we estimate that the octopus and squid lineages diverged ∼270 Mya, emphasizing the deep evolutionary history of coleoid cephalopods8,30 (Supplementary Note 7.1 and Extended Data Fig. 10a). Our analyses found hundreds of coleoid- and octopus-specific genes, many of which were expressed in tissues containing novel structures, including the chromatophore-laden skin, the suckers and the nervous system (Extended Data Fig. 10 and Supplementary Note 11). Taken together, these novel genes, the expansion of C2H2 ZNFs, genome rearrangements, and extensive transposable element activity yield a new landscape for both trans- and cis-regulatory elements in the octopus genome, resulting in changes in an otherwise ‘typical’ lophotrochozoan gene complement that contributed to the evolution of cephalopod neural complexity and morphological innovations.
Genome and transcriptome sequence reads are deposited in the SRA as BioProjects PRJNA270931 and PRJNA285380. The genome assembly and annotation are linked to the same BioProject ID. A browser of this genome assembly is available at (http://octopus.metazome.net/).
Genome sequencing and assembly
Genomic DNA from a single male Octopus bimaculoides31 was isolated and sequenced using Illumina technology to 60-fold redundant coverage in libraries spanning a range of pairs from ∼350 bp to 10 kilobases (kb). These data were assembled with meraculous32 achieving a contig N50-length of 5.4 kb and a scaffold N50-length of 470 kb. The longest scaffold contains 99 genes and half of all predicted genes are on scaffolds with 8 or more genes (Supplementary Note 1).
Genome size and heterozygosity
The O. bimaculoides haploid genome size was estimated to be ∼2.7 gigabases (Gb) based on fluorescence (2.66–2.68 Gb) and k-mer (2.86 Gb) measurements (Supplementary Notes 1 and 2), making it several times larger than other sequenced molluscan and lophotrochozoan genomes17. We observed nucleotide-level heterozygosity within the sequenced genome to be 0.08%, which may reflect a small effective population size relative to broadcast-spawning marine invertebrates.
Twelve transcriptomes were sequenced from RNA isolated from ova, testes, viscera, posterior salivary gland (PSG), suckers, skin, developmental stage 15 (St15)33, retina, optic lobe (OL), supraesophageal brain (Supra), subesophageal brain (Sub), and axial nerve cord (ANC) (Supplementary Note 2). RNA was isolated using TRIzol (Invitrogen) and 100-bp paired-end reads (insert size 300 bp) were generated on an Illumina HiSeq2000 sequencing machine.
De novo transcriptome assembly
Adapters and low-quality reads were removed before assembling transcriptomes using the Trinity de novo assembly package (version r2013-02-25 (refs 34, 35)). Assembly statistics are summarized in Supplementary Table 2.2. Following assembly, peptide-coding regions were translated using TransDecoder in the Trinity package. We compared the de novo assembled RNA-seq output to the genome to evaluate the completeness of the genome assembly. To minimize the number of spuriously assembled transcripts, only transcripts with ORFs predicted by TransDecoder were mapped onto the genome with BLASTN. Only 1,130 out of 48,259 transcripts with ORFs (2.34%) did not have a match in the genome with a minimum identity of 95%.
Annotation of transposable elements
Transposable elements were identified with RepeatScout and RepeatModeler36, and the masking was done with RepeatMasker37, as outlined in Supplementary Note 4.2. The most abundant transposable element is a previously identified octopus-specific SINE38 that accounts for 4% of the assembled genome.
Annotation of protein-coding genes
Protein-coding genes were annotated by combining transcriptome evidence with homology-based and de novo gene prediction methods (Supplementary Note 4). For homology prediction we used predicted peptide sets of three previously sequenced molluscs (L. gigantea, C. gigas, and A. californica) along with selected other metazoans. Alternative splice isoforms were identified with PASA39. Annotation statistics are provided in Supplementary Table 4.1.1. Genes known in vertebrates to have many isoforms, such as ankyrin, TRAK1 and LRCH1, also show alternative splicing in octopus but at a more limited level. Octopus genes with ten or more alternative splice forms are provided in Supplementary Table 4.1.2.
Calibration of sequence divergence with respect to time
The divergence between squid and octopus was estimated using r8s40 by fixing cephalopod divergence from bivalves and gastropods to 540 Mya8. Our estimate of 270 Mya for the squid–octopus divergence corresponds to mean neutral substitution rate of dS ∼2 based on the protein-directed CDS alignments between the species (Supplementary Fig. 6.1.2) and a dS estimation using the yn00 program41. Throughout the manuscript we convert from sequence divergence to time by assuming that dS ∼1 corresponds to 135 million years. For example, unlinked octopus protocadherins appear to have expanded ∼135 Mya based on mean pairwise dS ∼1, after octopuses diverged from squid. In contrast, clustered octopus protocadherins are much more similar in sequence (mean pairwise dS ∼0.4, or ∼55 Mya).
Quantifying gene expression
Transcriptome reads were mapped to the genome assembly with TopHat 2.0.11 (ref. 42). A range of 76–90% of reads from the different samples mapped to the genome. Mapped reads were sorted and indexed with SAMtools43. The read counts in each tissue were produced with BEDTools multicov program44 using the gene model coordinates. The counts were normalized by the total transcriptome size of each tissue and by the length of the gene. Heat maps showing expression patterns were generated in R using the heatmap.2 function.
Gene families of particular interest, including developmental regulatory genes, neural-related genes, and gene families that appear to be expanded in O. bimaculoides, were manually curated and analysed. We searched the octopus genome and transcriptome assemblies using BLASTP and TBLASTN with annotated sequences from human, mouse and D. melanogaster. Bulk analyses were also performed using Pfam45 and PANTHER46. We used BLASTP and TBLASTX to search for specific gene families in deposited genome and transcriptome databases for L. gigantea, A. californica, C. gigas, C. teleta, T. castaneum, D. melanogaster, C. elegans, N. vectensis, A. queenslandica, S. kowalevskii, B. floridae, C. intestinalis, D. rerio, M. musculus and H. sapiens. Candidate genes were verified with BLAST47 and Pfam45 analysis. Genes identified in the octopus genome were confirmed and extended using the transcriptomes. Multiple gene models that matched the same transcript were combined. The identified sequences from octopus and other bilaterians were aligned with either MUSCLE48, CLUSTALO49, MacVector 12.6 (MacVector, North Carolina), or Jalview50. Phylogenetic trees were constructed with FastTree51 using the Jones–Taylor–Thornton model of amino acid evolution, and visualized with FigTree v1.3.1.
Microsynteny was computed based on metazoan node gene families (Supplementary Note 7). We used Nmax 10 (maximum of 10 intervening genes) and Nmin 3 (minimum of three genes in a syntenic block) according to the pipeline described in ref. 17 (Supplementary Note 6). To simplify gene family assignments we limited our analyses to 4,033 gene families shared among human, amphioxus, Capitella, Helobdella, Octopus, Lottia, Crassostrea, Drosophila and Nematostella. We required ancestral bilaterian syntenic blocks to have a minimum of one species present in both ingroups, or in one ingroup and one outgroup. To examine the effect of fragmented genome assemblies, we simulated shorter assemblies by artificially fragmenting genomes to contain on average 5 genes per scaffold (Supplementary Note 6).
In comparison with other bilaterian genomes, we find that the octopus genome is substantially rearranged. In looking at microsyntenic linkages of genes with a maximum of 10 intervening genes, we found that octopus conserves only 34 out of 198 ancestral bilaterian microsyntenic blocks; the limpet Lottia and amphioxus retain more than twice as many such linkages (96 and 140, respectively). This difference remains significant after accounting for genes missed through orthology assignment as well as simulations of shorter scaffold sizes (Supplementary Note 6; Extended Data Fig. 9b). Scans for intra-genomic synteny, and doubly conserved synteny with Lottia, were performed as described in Supplementary Note 6.
Transposable elements and synteny dynamics
The 5 kb upstream and downstream regions of genes were surveyed for transposable element (TE) content. For genes with non-zero TE load, their assignment to either conserved or lost bilaterian synteny in octopus was done using the microsynteny calculation described above. The number of genes for each category and TE class were as follows: 484 genes for retained synteny and 1,193 genes in lost synteny for all TE classes; 440 and 1,107, respectively, for SINEs; and 116 and 290, respectively, for Mariner. Wilcoxon U-tests for the difference of TE load in linked versus non-linked genes were conducted in R.
To assess transposon activity we assigned transcriptome reads aligned to 5,496,558 annotated transposon loci using BEDTools44. Of these, 2,685,265 loci showed expression in at least one of the tissues.
RNA-seq reads were mapped to the genome with TopHat52, and SAMtools43 was used to identify SNPs between the genomic and the RNA sequences. To identify polymorphic positions in the genome, SNPs and indels were predicted using GATK HaplotypeCaller version 3.1-1 in discovery mode with a minimum Phred scaled probability score of 30, based on an alignment of the 350 bp and 500 bp genomic fragment libraries using BWA-MEM version 0.7.6a. Using BEDTools44, we removed SNPs predicted in both the transcriptome and the genome and discarded SNPs that had a Phred score below 40 or were outside of predicted genes. SNPs were binned according to the type of nucleotide change and the direction of transcription. Candidate edited genes were taken as those having SNPs with A-to-G substitutions in the predicted mRNA transcripts.
Cephalopod novelties were obtained by BLASTP and TBLASTN searches against the whole NR database53 and a custom database of several mollusc transcriptomes (Supplementary Note 11.1). To ensure that we had as close to full-length sequence as possible, we extended proteins predicted from octopus genomic sequence with our de novo assembled transcriptomes, using the longest match to query NR, transcriptome and EST sequences from other animals. Gene sequences with transcriptome support but without a match to non-cephalopod animals at an e-value cutoff of 1 × 10−3 were considered for further analysis. Octopus sequences with a match of 1 × 10−5 or better to a sequence from another cephalopod were used to construct gene families, which were characterized by their BLAST alignments, HMM, PFAM-A/B, and UNIREF90 hits. The cephalopod-specific gene families are listed in the Source Data file for Extended Data Fig. 10. Octopus-specific novelties were defined as sequences with transcriptome support but without any matches to sequences from any other animals (<1 × 10−3), including nautiloid and decapodiform cephalopods.
Sequence Read Archive
We thank C. T. Brown and J. Rosenthal for making Doryteuthis RNA-seq data available before publication; C. Ha, J. Orenstein, J. Brandenburger, M. Glotzer and H. Gui for bioinformatic assistance; S. Shigeno for help with tissue dissection; C. Huffard and R. Caldwell for providing the O. bimaculoides specimen used for genomic DNA isolation; and E. Begovic for genomic DNA preparation. This work was supported by the Molecular Genetics Unit of the Okinawa Institute of Science and Technology Graduate University (S.B. and D.S.R.) and by funding from the NSF (IOS-1354898) and NIH (R03 HD064887) to C.W.R. and from the NSF (DGE-0903637) to Z.Y.W. This work used the Vincent J. Coates Genomics Sequencing Laboratory at UC Berkeley, supported by NIH S10 instrumentation grants S10RR029668 and S10RR027303, and the University of Chicago Functional Genomics Facility, supported by NIH grant UL1 TR000430.
Extended data figures
The ability to camouflage is present throughout the animal kingdom, but cephalopods uniquely achieve adaptive coloration. Three different structures in the skin contribute to the color of an octopus: chromatophores, iridophores, and leucophores. The reflectin family of proteins reversibly regulates iridescence. Muscles in the skin allow octopuses to alter texture and disrupt the visual boundaries of the body. Most importantly, this complex behavior happens very quickly, allowing octopuses to change their appearance almost instantaneously. The O. bimaculoides genome and transcriptome is reported by Albertin and Simakov et al. Credit: Z. Yan Wang, C. Ragsdale, J. Reynolds, and Schadenfreude the octopus.
Octopuses are perhaps most easily recognized by their eight appendages. The arms are strong muscular hydrostats, each covered with hundreds of suckers that allow octopuses to exert powerful suction forces. The flexibility and strength of octopus arms is a unique morphological innovation in the animal kingdom. The O. bimaculoides genome and transcriptome is reported by Albertin and Simakov et al. Credit: Z. Yan Wang, C. Ragsdale, J. Reynolds, and Schadenfreude the octopus.
About this article
Molecular cloning and transcriptional analysis of a NPY receptor-like in common Chinese cuttlefish Sepiella japonica
Journal of Oceanology and Limnology (2018)