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CLASP-mediated cortical microtubule organization guides PIN polarization axis

Nature volume 495, pages 529–533 (2013)Cite this article

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A Retraction to this article was published on 19 March 2014

Abstract

Recent evidence indicates a correlation between orientation of the plant cortical microtubule cytoskeleton and localization of polar cargoes1. However, the molecules and mechanisms that create this correlation have remained unknown. Here we show that, in Arabidopsis thaliana, the microtubule orientation regulators CLASP2,3 and MAP65 (refs 3, 4) control the abundance of polarity regulator PINOID kinase5,6 at the plasma membrane. By localized upregulation of clathrin-dependent endocytosis7 at cortical microtubule- and clathrin-rich domains orthogonal to the axis of polarity, PINOID accelerates the removal of auxin transporter PIN proteins8,9,10 from those sites. This mechanism links directional microtubule organization to the polar localization of auxin transporter PIN proteins, and clarifies how microtubule-enriched cell sides are kept distinct from polar delivery domains. Our results identify the molecular machinery that connects microtubule organization to the regulation of the axis of PIN polarization.

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Figure 1: PIN2 localization and trafficking is altered in the clasp-1 mutant.
Figure 2: PINOID abundance is enhanced in the clasp-1 mutant.
Figure 3: Endocytosis is enhanced in clasp-1 mutants by a PID-dependent increment in plasma-membrane localized clathrin.
Figure 4: Microtubule orientation defines the axis of PIN polarization.

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Acknowledgements

We thank M. Estelle, G. Wasteneys, K. Hayashi, C. Luschnig, I. Hwang, M. Sasabe and M. Heisler for sharing published material. We thank F. Kindt and R. Leito for photography and image processing, and the Akhmanova laboratory for the western blotting facility. This work was supported by the Utrecht University’s Starting Independent Investigator, the Netherlands Organisation for Scientific Research VIDI and European Research Council Starting Investigator grants to P.D., and the European Research Council Advanced Investigator grant, the Netherlands Organisation for Scientific Research Spinoza and Netherlands Proteomics Centre grants to B.S. K.K. was recruited from grants of P.D., and H.Z. was supported by grants of B.S.

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Authors and Affiliations

  1. Department of Biology, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands,

    Klementina Kakar, Hongtao Zhang, Ben Scheres & Pankaj Dhonukshe

  2. Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Padualaan 8, 3584CH Utrecht, The Netherlands ,

    Hongtao Zhang

  3. Netherlands Proteomics Centre, Padualaan 8, 3584CH Utrecht, The Netherlands ,

    Hongtao Zhang & Ben Scheres

  4. Wageningen University Research, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands ,

    Ben Scheres

  5. Department of Plant Systems Biology, VIB, Technologiepark 927, B-9052 Ghent, Belgium,

    Pankaj Dhonukshe

  6. Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium,

    Pankaj Dhonukshe

Authors
  1. Klementina Kakar
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  2. Hongtao Zhang
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Contributions

P.D. conceptualized the project, designed the experiments and directed the work; B.S. contributed to the experimental design; K.K. and P.D. performed experiments and analysed data; H.Z. contributed to western analysis; P.D., K.K. and B.S. wrote the paper.

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Correspondence to Pankaj Dhonukshe.

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Kakar, K., Zhang, H., Scheres, B. et al. CLASP-mediated cortical microtubule organization guides PIN polarization axis. Nature 495, 529–533 (2013). https://doi.org/10.1038/nature11980

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