Letter | Published:

Autoregulation of microRNA biogenesis by let-7 and Argonaute

Nature volume 486, pages 541544 (28 June 2012) | Download Citation


MicroRNAs (miRNAs) comprise a large family of small RNA molecules that post-transcriptionally regulate gene expression in many biological pathways1. Most miRNAs are derived from long primary transcripts that undergo processing by Drosha to produce 65-nucleotide precursors that are then cleaved by Dicer, resulting in the mature 22-nucleotide forms2,3. Serving as guides in Argonaute protein complexes, mature miRNAs use imperfect base pairing to recognize sequences in messenger RNA transcripts, leading to translational repression and destabilization of the target messenger RNAs4,5. Here we show that the miRNA complex also targets and regulates non-coding RNAs that serve as substrates for the miRNA-processing pathway. We found that the Argonaute protein in Caenorhabditis elegans, ALG-1, binds to a specific site at the 3′ end of let-7 miRNA primary transcripts and promotes downstream processing events. This interaction is mediated by mature let-7 miRNA through a conserved complementary site in its own primary transcript, thus creating a positive-feedback loop. We further show that ALG-1 associates with let-7 primary transcripts in nuclear fractions. Argonaute also binds let-7 primary transcripts in human cells, demonstrating that the miRNA pathway targets non-coding RNAs in addition to protein-coding messenger RNAs across species. Moreover, our studies in C. elegans reveal a novel role for Argonaute in promoting biogenesis of a targeted transcript, expanding the functions of the miRNA pathway in gene regulation. This discovery of autoregulation of let-7 biogenesis establishes a new mechanism for controlling miRNA expression.

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We thank J. Lykke-Andersen and members of the Pasquinelli lab for reading the manuscript, and we thank D. Hogan for discussions. We thank F. Slack for originally pointing out the let-7 complementary site in pri-let-7; the M. David lab for sharing their real-time PCR machine; P. Van Wynsberghe for the Δalg-1 primary let-7 plasmid; C. Mello for the worm fractionation protocol; E. Moss for LIN-28 antibodies; and A. Gorin, H. Jenq and S. Verma for technical assistance. Funding was provided by a Leukemia & Lymphoma Society Special Fellow Award 3611-11 (D.G.Z.); US National Institutes of Health (NIH) CMG and NIH/NCI T32 CA009523 Training Grants (Z.S.K.); the Swedish Board of Study Support (R.K.C.); and NIH grant GM071654, the Keck Foundation and the Peter Gruber Foundation (A.E.P.).

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Author notes

    • Dimitrios G. Zisoulis
    •  & Zoya S. Kai

    These authors contributed equally to this work.


  1. Division of Biology, University of California, San Diego, La Jolla, California 92093-0349, USA

    • Dimitrios G. Zisoulis
    • , Zoya S. Kai
    • , Roger K. Chang
    •  & Amy E. Pasquinelli
  2. Department of Biology Education, Stockholm University, 106 91 Stockholm, Sweden

    • Roger K. Chang


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A.E.P., D.G.Z. and Z.S.K. designed the project and wrote the paper. D.G.Z. (Figs 1b, c, 2a, 3b, g and 4b–e and Supplementary Figs 2, 3a–c, 4a and 6a), Z.S.K. (Figs 1d, 2c, d and 3c and Supplementary Figs 3d, 4c, d, g and 6b), R.K.C. (Figs 2b, 3g and 4a and Supplementary Fig. 4b) and A.E.P. (Fig. 4a and Supplementary Fig. 7) performed the experiments and analysed the data. A.E.P. supervised the studies.

Competing interests

The authors declare no competing financial interests.

Corresponding author

Correspondence to Amy E. Pasquinelli.

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