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Coherently wired light-harvesting in photosynthetic marine algae at ambient temperature


Photosynthesis makes use of sunlight to convert carbon dioxide into useful biomass and is vital for life on Earth. Crucial components for the photosynthetic process are antenna proteins, which absorb light and transmit the resultant excitation energy between molecules to a reaction centre. The efficiency of these electronic energy transfers has inspired much work on antenna proteins isolated from photosynthetic organisms to uncover the basic mechanisms at play1,2,3,4,5. Intriguingly, recent work has documented6,7,8 that light-absorbing molecules in some photosynthetic proteins capture and transfer energy according to quantum-mechanical probability laws instead of classical laws9 at temperatures up to 180 K. This contrasts with the long-held view that long-range quantum coherence between molecules cannot be sustained in complex biological systems, even at low temperatures. Here we present two-dimensional photon echo spectroscopy10,11,12,13 measurements on two evolutionarily related light-harvesting proteins isolated from marine cryptophyte algae, which reveal exceptionally long-lasting excitation oscillations with distinct correlations and anti-correlations even at ambient temperature. These observations provide compelling evidence for quantum-coherent sharing of electronic excitation across the 5-nm-wide proteins under biologically relevant conditions, suggesting that distant molecules within the photosynthetic proteins are ‘wired’ together by quantum coherence for more efficient light-harvesting in cryptophyte marine algae.

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Figure 1: Structure and spectroscopy of cryptophyte antenna proteins.
Figure 2: Two-dimensional photon echo data for PC645.
Figure 3: Two-dimensional photon echo data for PE545.


  1. Green, B. R. & Parson, W. W. eds. Light-Harvesting Antennas in Photosynthesis (Kluwer, Dordrecht, 2003)

    Book  Google Scholar 

  2. Scholes, G. D. Long-range resonance energy transfer in molecular systems. Annu. Rev. Phys. Chem. 54, 57–87 (2003)

    ADS  CAS  Article  Google Scholar 

  3. Jang, S., Newton, M. D. & Silbey, R. J. Multichromophoric Förster resonance energy transfer from B800 to B850 in the light harvesting complex 2: evidence for subtle energetic optimization by purple bacteria. J. Phys. Chem. B 111, 6807–6814 (2007)

    CAS  Article  Google Scholar 

  4. Cheng, Y. C. & Fleming, G. R. Dynamics of light harvesting in photosynthesis. Annu. Rev. Phys. Chem. 60, 241–262 (2009)

    ADS  CAS  Article  Google Scholar 

  5. van Grondelle, R. & Novoderezhkin, V. I. Energy transfer in photosynthesis: experimental insights and quantitative models. Phys. Chem. Chem. Phys. 8, 793–807 (2006)

    CAS  Article  Google Scholar 

  6. Engel, G. S. et al. Evidence for wavelike energy transfer through quantum coherence in photosynthetic systems. Nature 446, 782–786 (2007)

    ADS  CAS  Article  Google Scholar 

  7. Lee, H., Cheng, Y. C. & Fleming, G. R. Coherence dynamics in photosynthesis: protein protection of excitonic coherence. Science 316, 1462–1465 (2007)

    ADS  CAS  Article  Google Scholar 

  8. Mercer, I. P. et al. Instantaneous mapping of coherently coupled electronic transitions and energy transfers in a photosynthetic complex using angle-resolved coherent optical wave-mixing. Phys. Rev. Lett. 102, 057402 (2009)

    ADS  Article  Google Scholar 

  9. Feynman, R. P. Space-time approach to non-relativistic quantum mechanics. Rev. Mod. Phys. 20, 367–387 (1948)

    ADS  MathSciNet  Article  Google Scholar 

  10. Jonas, D. M. Two-dimensional femtosecond spectroscopy. Annu. Rev. Phys. Chem. 54, 425–463 (2003)

    ADS  CAS  Article  Google Scholar 

  11. Brixner, T., Mancal, T., Stiopkin, I. V. & Fleming, G. R. Phase-stabilized two-dimensional electronic spectroscopy. J. Chem. Phys. 121, 4221–4236 (2004)

    ADS  CAS  Article  Google Scholar 

  12. Cho, M. H. Coherent two-dimensional optical spectroscopy. Chem. Rev. 108, 1331–1418 (2008)

    CAS  Article  Google Scholar 

  13. Abramavicius, D. et al. Coherent multidimensional optical spectroscopy of excitons in molecular aggregates; quasiparticle versus supermolecule perspectives. Chem. Rev. 109, 2350–2408 (2009)

    CAS  Article  Google Scholar 

  14. Spear-Bernstein, L. & Miller, K. R. Unique location of the phycobiliprotein light-harvesting pigment in the cryptophyceae. J. Phycol. 25, 412–419 (1989)

    CAS  Article  Google Scholar 

  15. van der Weij-De Wit, C. D. et al. Phycocyanin sensitizes both photosystem I and photosystem II in cryptophyte Chroomonas CCMP270 cells. Biophys. J. 94, 2423–2433 (2008)

    CAS  Article  Google Scholar 

  16. Wilk, K. E. et al. Evolution of a light-harvesting protein by addition of new subunits and rearrangement of conserved elements: crystal structure of a cryptophyte phycoerythrin at 1.63-Ångstrom resolution. Proc. Natl Acad. Sci. USA 96, 8901–8906 (1999)

    ADS  CAS  Article  Google Scholar 

  17. Wedemayer, G. J., Kidd, D. G., Wemmer, D. E. & Glazer, A. N. Phycobilins of cryptophycean algae: occurrence of dihydrobiliverdin and mesobiliverdin in cryptomonad biliproteins. J. Biol. Chem. 267, 7315–7331 (1992)

    CAS  PubMed  Google Scholar 

  18. Mirkovic, T. et al. Ultrafast light harvesting dynamics in the cryptophyte phycocyanin 645. Photochem. Photobiol. Sci. 6, 964–975 (2007)

    CAS  PubMed  Google Scholar 

  19. Scholes, G. D. & Rumbles, G. Excitons in nanoscale systems. Nature Mater. 5, 683–696 (2006)

    ADS  CAS  Article  Google Scholar 

  20. Doust, A. B. et al. Developing a structure-function model for the cryptophyte phycoerythrin 545 using ultrahigh resolution crystallography and ultrafast laser spectroscopy. J. Mol. Biol. 344, 135–153 (2004)

    ADS  CAS  Article  Google Scholar 

  21. Scholes, G. D. et al. How solvent controls electronic energy transfer and light harvesting. J. Phys. Chem. B 111, 6978–6982 (2007)

    CAS  Article  Google Scholar 

  22. Rhodes, W. Radiationless transitions in isolated molecules. the effects of molecular size and radiation bandwidth. J. Chem. Phys. 50, 2885–2896 (1969)

    ADS  CAS  Article  Google Scholar 

  23. Cheng, Y. C. & Fleming, G. R. Coherence quantum beats in two-dimensional electronic spectroscopy. J. Phys. Chem. A 112, 4254–4260 (2008)

    CAS  Article  Google Scholar 

  24. Rackovsky, S. & Silbey, R. Electronic-energy transfer in impure solids. 1. 2 molecules embedded in a lattice. Mol. Phys. 25, 61–72 (1973)

    ADS  CAS  Article  Google Scholar 

  25. Hwang, H. & Rossky, P. J. Electronic decoherence induced by intramolecular vibrational motions in a betaine dye molecule. J. Phys. Chem. B 108, 6723–6732 (2004)

    CAS  Article  Google Scholar 

  26. Franco, I., Shapiro, M. & Brumer, P. Femtosecond dynamics and laser control of charge transport in trans-polyacetylene. J. Chem. Phys. 128, 244905 (2008)

    ADS  Article  Google Scholar 

  27. Collini, E. & Scholes, G. D. Coherent intrachain energy migration in a conjugated polymer at room temperature. Science 323, 369–373 (2009)

    ADS  CAS  Article  Google Scholar 

  28. Beljonne, D., Curutchet, C., Scholes, G. D. & Silbey, R. Beyond Förster resonance energy transfer in biological and nanoscale systems. J. Phys. Chem. B 113, 6583–6599 (2009)

    CAS  Article  Google Scholar 

  29. Langhoff, C. A. & Robinson, G. W. Time decay and untangling of vibronically tangled resonances: naphthalene second singlet. Chem. Phys. 6, 34–53 (1974)

    CAS  Article  Google Scholar 

  30. Jang, S. Theory of coherent resonance energy transfer for coherent initial condition. J. Chem. Phys. 131, 164101 (2009)

    ADS  Article  Google Scholar 

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This work was supported by the Natural Sciences and Engineering Research Council of Canada and the Australian Research Council. G.D.S. acknowledges the support of an EWR Steacie Memorial Fellowship.

Author Contributions E.C. performed the experiments on PC645 and analysed those data. C.Y.W. performed the experiments on PE545 and analysed those data. K.E.W. prepared the samples. P.M.G.C. and G.D.S. designed the research. P.B. and G.D.S. examined the interpretation of the results. G.D.S. wrote the paper. All authors discussed the results and commented on the manuscript.

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Correspondence to Gregory D. Scholes.

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Collini, E., Wong, C., Wilk, K. et al. Coherently wired light-harvesting in photosynthetic marine algae at ambient temperature. Nature 463, 644–647 (2010).

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