Uncovering the origins of myocardial cells is important for understanding and treating heart diseases1,2. Cai et al.3 suggest that Tbx18-expressing epicardium provides a substantial contribution to myocytes in the ventricular septum and the atrial and ventricular walls. Here we show that the T-box transcription factor gene 18 (Tbx18) itself is expressed in the myocardium, showing that their genetic lineage tracing system does not allow conclusions of an epicardial origin of cardiomyocytes in vivo to be drawn.
Subscribe to Journal
Get full journal access for 1 year
only $3.83 per issue
All prices are NET prices.
VAT will be added later in the checkout.
Rent or Buy article
Get time limited or full article access on ReadCube.
All prices are NET prices.
Olson, E. N. A decade of discoveries in cardiac biology. Nature Med. 10, 467–474 (2004)
Murry, C. E., Field, L. J. & Menasche, P. Cell-based cardiac repair: reflections at the 10-year point. Circulation 112, 3174–3183 (2005)
Cai, C. L. et al. A myocardial lineage derives from Tbx18 epicardial cells. Nature 454, 104–108 (2008)
Mikawa, T. & Fischman, D. A. Retroviral analysis of cardiac morphogenesis: discontinuous formation of coronary vessels. Proc. Natl Acad. Sci. USA 89, 9504–9508 (1992)
Mikawa, T. & Gourdie, R. G. Pericardial mesoderm generates a population of coronary smooth muscle cells migrating into the heart along with ingrowth of the epicardial organ. Dev. Biol. 174, 221–232 (1996)
Winter, E. M. & Gittenberger-de Groot, A. C. Epicardium-derived cells in cardiogenesis and cardiac regeneration. Cell. Mol. Life Sci. 64, 692–703 (2007)
Yamauchi, Y. et al. A novel transgenic technique that allows specific marking of the neural crest cell lineage in mice. Dev. Biol. 212, 191–203 (1999)
Kraus, F., Haenig, B. & Kispert, A. Cloning and expression analysis of the mouse T-box gene Tbx18. Mech. Dev. 100, 83–86 (2001)
Buckingham, M., Meilhac, S. & Zaffran, S. Building the mammalian heart from two sources of myocardial cells. Nature Rev. Genet. 6, 826–835 (2005)
Franco, D. et al. Left and right ventricular contributions to the formation of the interventricular septum in the mouse heart. Dev. Biol. 294, 366–375 (2006)
Bakker, M. L. et al. Transcription factor Tbx3 is required for the specification of the atrioventricular conduction system. Circ. Res. 102, 1340–1349 (2008)
Soriano, P. Generalized lacZ expression with the ROSA26 Cre reporter strain. Nature Genet. 21, 70–71 (1999)
Kreidberg, J. A., Sariola, H., Loring, J. M., Maeda, M. & Pelletier, J. Housman, D. Jaenisch, R. WT-1 is required for early kidney development. Cell 74, 679–691 (1993)
Moore, A. W., McInnes, L., Kreidberg, J., Hastie, N. D. & Schedl, A. YAC complementation shows a requirement for Wt1 in the development of epicardium, adrenal gland and throughout nephrogenesis. Development 126, 1845–1857 (1999)
Zhou, B. et al. Epicardial progenitors contribute to the cardiomyocyte lineage in the developing heart. Nature 454, 109–113 (2008)
About this article
Cite this article
Christoffels, V., Grieskamp, T., Norden, J. et al. Tbx18 and the fate of epicardial progenitors. Nature 458, E8–E9 (2009). https://doi.org/10.1038/nature07916
Circulation Research (2020)
Developmental Cell (2020)
Canonical Wnt signaling promotes pacemaker cell specification of cardiac mesodermal cells derived from mouse and human embryonic stem cells
STEM CELLS (2019)
Developmental Cell (2019)