Letter | Published:

Peptide neurotransmitters activate a cation channel complex of NALCN and UNC-80

Nature volume 457, pages 741744 (05 February 2009) | Download Citation

Abstract

Several neurotransmitters act through G-protein-coupled receptors to evoke a ‘slow’ excitation of neurons1,2. These include peptides, such as substance P and neurotensin, as well as acetylcholine and noradrenaline. Unlike the fast (approximately millisecond) ionotropic actions of small-molecule neurotransmitters, the slow excitation is not well understood at the molecular level, but can be mainly attributed to suppressing K+ currents and/or activating a non-selective cation channel3,4,5,6,7,8,9. The molecular identity of this cation channel has yet to be determined; similarly, how the channel is activated and its relative contribution to neuronal excitability induced by the neuropeptides are unknown. Here we show that, in the mouse hippocampal and ventral tegmental area neurons, substance P and neurotensin activate a channel complex containing NALCN and a large previously unknown protein UNC-80. The activation by substance P through TACR1 (a G-protein-coupled receptor for substance P) occurs by means of a unique mechanism: it does not require G-protein activation but is dependent on Src family kinases. These findings identify NALCN as the cation channel activated by substance P receptor, and suggest that UNC-80 and Src family kinases, rather than a G protein, are involved in the coupling from receptor to channel.

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Accessions

Primary accessions

GenBank/EMBL/DDBJ

Data deposits

The sequence of mUNC-80 is deposited in GenBank under accession number FJ210934.

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Acknowledgements

We thank D. Clapham, C. Deutsch, I. Medina, B. Novarro, M. Schmidt and H. Xu for critically reading earlier versions of the manuscript, J. Xia for help with experiments, H. Yu and L. Yue for cDNA constructs, and Sanofi-Aventis for the gift of SR48692. This work was supported, in part, by funding from American Heart Association, the NIH and the University of Pennsylvania Research Foundation.

Author Contributions B.L. did recordings from neurons (Figs 13 and Supplementary Fig. 2) and all the HEK293T cells (Fig. 4 and Supplementary Figs 1 and 7–10). Y.S. contributed to neuronal recordings (Figs 1 and 2 and Supplementary Figs 3 and 4). S.D. contributed to work in Fig. 2. H.W., Y.W. and J.L. did the protein work (Fig. 4 and Supplementary Fig. 6). D.R. started the project, designed experiments and developed the cDNA constructs. B.L. and D.R. wrote the paper.

Author information

Author notes

    • Boxun Lu
    •  & Yanhua Su

    These authors contributed equally to this work.

    • Yanhua Su

    Present address: State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China.

Affiliations

  1. Department of Biology, University of Pennsylvania, 415 S. University Avenue, Philadelphia, Pennsylvania 19104, USA

    • Boxun Lu
    • , Yanhua Su
    • , Sudipto Das
    • , Haikun Wang
    • , Yan Wang
    • , Jin Liu
    •  & Dejian Ren

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Correspondence to Dejian Ren.

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DOI

https://doi.org/10.1038/nature07579

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