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Histone arginine methylation regulates pluripotency in the early mouse embryo


It has been generally accepted that the mammalian embryo starts its development with all cells identical, and only when inside and outside cells form do differences between cells first emerge. However, recent findings show that cells in the mouse embryo can differ in their developmental fate and potency as early as the four-cell stage1,2,3,4. These differences depend on the orientation and order of the cleavage divisions that generated them2,5. Because epigenetic marks are suggested to be involved in sustaining pluripotency6,7, we considered that such developmental properties might be achieved through epigenetic mechanisms. Here we show that modification of histone H3, through the methylation of specific arginine residues, is correlated with cell fate and potency. Levels of H3 methylation at specific arginine residues are maximal in four-cell blastomeres that will contribute to the inner cell mass (ICM) and polar trophectoderm and undertake full development when combined together in chimaeras. Arginine methylation of H3 is minimal in cells whose progeny contributes more to the mural trophectoderm and that show compromised development when combined in chimaeras. This suggests that higher levels of H3 arginine methylation predispose blastomeres to contribute to the pluripotent cells of the ICM. We confirm this prediction by overexpressing the H3-specific arginine methyltransferase CARM1 in individual blastomeres and show that this directs their progeny to the ICM and results in a dramatic upregulation of Nanog and Sox2. Thus, our results identify specific histone modifications as the earliest known epigenetic marker contributing to development of ICM and show that manipulation of epigenetic information influences cell fate determination.

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Figure 1: Levels of H3R26me are different in blastomeres of four-cell-stage embryos and correlate with their spatial arrangement.
Figure 2: The ‘vegetal’ blastomere in ME embryos has the lowest levels of H3R26me.
Figure 3: CARM1 overexpression in a two-cell blastomere results in the contribution of that cell predominantly to the ICM.
Figure 4: Overexpression of CARM1 results in elevated levels of arginine methylation and upregulation of Nanog and Sox2.


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We thank P. Greda for bead labelling, C. Lee for assistance, D. Glover for comments on the manuscript, M. Stallcup for the CARM1 expression vectors, and M. Bedford for providing the Carm1-/- MEFs and the PRMT inhibitor. M.-E.T.-P. is an EMBO long-term fellow. We are grateful to the Wellcome Trust Senior Fellowship to M.Z.-G., which supported this work.

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Correspondence to Magdalena Zernicka-Goetz.

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Torres-Padilla, ME., Parfitt, DE., Kouzarides, T. et al. Histone arginine methylation regulates pluripotency in the early mouse embryo. Nature 445, 214–218 (2007).

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