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Low dose oral cannabinoid therapy reduces progression of atherosclerosis in mice

Nature volume 434, pages 782786 (07 April 2005) | Download Citation

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  • A Corrigendum to this article was published on 26 May 2005

Abstract

Atherosclerosis is a chronic inflammatory disease, and is the primary cause of heart disease and stroke in Western countries1. Derivatives of cannabinoids such as delta-9-tetrahydrocannabinol (THC) modulate immune functions2 and therefore have potential for the treatment of inflammatory diseases. We investigated the effects of THC in a murine model of established atherosclerosis. Oral administration of THC (1 mg kg-1 per day) resulted in significant inhibition of disease progression. This effective dose is lower than the dose usually associated with psychotropic effects of THC. Furthermore, we detected the CB2 receptor (the main cannabinoid receptor expressed on immune cells2,3) in both human and mouse atherosclerotic plaques. Lymphoid cells isolated from THC-treated mice showed diminished proliferation capacity and decreased interferon-γ secretion. Macrophage chemotaxis, which is a crucial step for the development of atherosclerosis1, was also inhibited in vitro by THC. All these effects were completely blocked by a specific CB2 receptor antagonist4. Our data demonstrate that oral treatment with a low dose of THC inhibits atherosclerosis progression in the apolipoprotein E knockout mouse model, through pleiotropic immunomodulatory effects on lymphoid and myeloid cells. Thus, THC or cannabinoids with activity at the CB2 receptor may be valuable targets for treating atherosclerosis.

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Acknowledgements

This work was supported by grants from the Swiss National Science Foundation to F.M. and J.-L.F., from the Ernst and Lucie Schmidheiny Foundation (Geneva) to F.M., and from the Foundation for Medical Research (France) to C.A. The authors of this manuscript belong to the European Vascular Genomics Network (http://www.evgn.org), a Network of Excellence supported by the European Community's sixth Framework Programme for Research, Priority 1. We would like to thank M. Kosco-Vilbois for helpful discussion in preparing the manuscript, and M.-L. Bochaton-Piallat (Pathology Department) for technical advice.

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Author notes

    • Niels R. Veillard
    •  & Claire Arnaud

    These authors contributed equally to this work

Affiliations

  1. Division of Cardiology, Department of Medicine, Foundation for Medical Research, University Hospital, Faculty of Medicine, 1211 Geneva, Switzerland

    • Sabine Steffens
    • , Niels R. Veillard
    • , Claire Arnaud
    • , Graziano Pelli
    • , Fabienne Burger
    •  & François Mach
  2. Division of Gastroenterology, University Hospital, Faculty of Medicine, 1211 Geneva, Switzerland

    • Jean-Louis Frossard
  3. Institute of Legal Medicine, University Hospital, Faculty of Medicine, 1211 Geneva, Switzerland

    • Christian Staub
  4. Laboratory for Molecular Neurobiology, Department of Psychiatry, University of Bonn, 53105 Bonn, Germany

    • Andreas Zimmer

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The authors declare that they have no competing financial interests.

Corresponding author

Correspondence to François Mach.

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https://doi.org/10.1038/nature03389

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