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Transposon silencing in the Caenorhabditis elegans germ line by natural RNAi

Abstract

Transposable elements are stretches of DNA that can move and multiply within the genome of an organism. The Caenorhabditis elegans genome contains multiple Tc1 transposons that jump in somatic cells, but are silenced in the germ line1,2,3. Many mutants that have lost this silencing have also lost the ability to execute RNA interference (RNAi)2,3, a process whereby genes are suppressed by exposure to homologous double-stranded RNA (dsRNA). Here we show how RNAi causes transposon silencing in the nematode germ line. We find evidence for transposon-derived dsRNAs, in particular to the terminal inverted repeats, and show that these RNAs may derive from read-through transcription of entire transposable elements. Small interfering RNAs of Tc1 were detected. When a germline-expressed reporter gene is fused to a stretch of Tc1 sequence, this transgene is silenced in a manner dependent on functional mutator genes (mut-7, mut-16 and pk732). These results indicate that RNAi surveillance is triggered by fortuitous read-through transcription of dispersed Tc1 copies, which can form dsRNA as a result of ‘snap-back’ of the terminal inverted repeats. RNAi mediated by this dsRNA silences transposase gene expression.

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Acknowledgements

We thank R. Ketting for help in experiments and discussions, and E. Berezikov for help with the ballistic transformations. We acknowledge S. Fischer, N. Vastenhouw, V. Robert, E. Cuppen, R. May and M. Joosten for helpful discussions or for critically reading the manuscript. This work was supported by a VIDI fellowship from the Dutch Science Foundation (NWO) to T.S.

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Correspondence to Ronald H. A. Plasterk.

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The authors declare that they have no competing financial interests.

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Further reading

Figure 1: Analyses of Tc1 RNAs.
Figure 2: Analysis of Tc1 siRNAs.
Figure 3: Trans-inactivation of Tc1-containing transgenes.

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