MyD88 dependence of beryllium-induced dendritic cell trafficking and CD4+ T-cell priming

McKee, A S; Mack, D G; Crawford, F; Fontenot, A P

doi:10.1038/mi.2015.14

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Published: 11 March 2015

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MyD88 dependence of beryllium-induced dendritic cell trafficking and CD4⁺ T-cell priming

A S McKee¹,
D G Mack¹,
F Crawford^2,3 &
…
A P Fontenot^1,2

Mucosal Immunology volume 8, pages 1237–1247 (2015)Cite this article

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Abstract

Beryllium exposure results in beryllium hypersensitivity in a subset of exposed individuals, leading to granulomatous inflammation and fibrosis in the lung. In addition to its antigenic properties, beryllium has potent adjuvant activity that contributes to sensitization via unknown pathways. Here we show that beryllium induces cellular death and release of interleukin (IL)-1α and DNA into the lung. Release of IL-1α was inflammasome independent and required for beryllium-induced neutrophil recruitment into the lung. Beryllium enhanced classical dendritic cell (cDC) migration from the lung to draining lymph nodes (LNs) in an IL-1R-independent manner, and the accumulation of activated cDCs in the LN was associated with increased priming of CD4⁺ T cells. DC migration was reduced in Toll-like receptor 9 knockout (TLR9KO) mice; however, cDCs in the LNs of TLR9-deficient mice were highly activated, suggesting a role for more than one innate receptor in the effects on DCs. The adjuvant effects of beryllium on CD4⁺ T-cell priming were similar in wild-type, IL-1R-, caspase-1-, TLR2-, TLR4-, TLR7-, and TLR9-deficient mice. In contrast, DC migration, activation, and the adjuvant effects of beryllium were significantly reduced in myeloid differentiation primary response gene 88 knockout (MyD88KO) mice. Collectively, these data suggest that beryllium exposure results in the release of damage-associated molecular patterns that engage MyD88-dependent receptors to enhance pulmonary DC function.

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INTRODUCTION

Metal-induced T-cell hypersensitivities are common and occur in response to a wide range of metals.¹ The clinical manifestations of these hypersensitivities range from minor contact allergies to pulmonary fibrosis. Among the more serious hypersensitivities are those induced by beryllium and cobalt, which cause debilitating and life-threatening lung diseases. Chronic beryllium disease (CBD) occurs in genetically susceptible individuals who are exposed to beryllium in the workplace.^{2, 3} Beryllium is used in a variety of industries, and despite the implementation of strict exposure limits, CBD continues to occur in up to 16% of workers.⁴ The use of beryllium is increasing globally and more than one million workers worldwide have been exposed.³ In addition, the cost of treating a single CBD patient has been estimated to exceed $1 million.⁵ Thus CBD remains a costly and significant public health concern.

CBD is characterized by the presence of large numbers of effector memory T helper type 1 (Th1)-polarized CD4⁺ T cells in the lung that produce interferon-γ, interleukin (IL)-2 and tumor necrosis factor-α upon restimulation with beryllium in vitro.⁶ In contrast to sarcoidosis and other granulomatous lung disorders, there is no evidence for Th17- or Th2-polarized T cells in CBD.^{7, 8} In the majority of CBD patients, genetic susceptibility is related to the expression of specific HLA-DP alleles that express a glutamic acid at the 69th position of the β-chain.⁹ This polymorphic amino acid participates in coordination of the Be²⁺ cation within the major histocompatibility complex class II (MHCII) structure resulting in an altered conformation of the HLA-DP molecule loaded with particular self-peptides (HLA-DP/peptide complex).^{10, 11} Although beryllium does not directly contact the T-cell receptor, the modified HLA-DP2/peptide complex is recognized as a novel neoantigen by beryllium-specific T-cell receptors.¹¹ Once activated, beryllium-specific effector CD4⁺ T cells drive the development of granulomatous inflammation in the lung, which can progress to fibrosis, respiratory insufficiency, and death.¹²

Initiation of an effector CD4⁺ T-cell response is a complex process that involves not only the presence of a stimulatory MHCII/peptide complex but also requires additional signals from mature dendritic cells (DCs). DCs express innate pattern-recognition receptors (PRRs) that broadly recognize pathogen-associated molecular patterns encountered during infection and alarmins or damage-associated molecular patterns (DAMPs) released following cell death.¹³ PRRs drive the migration and maturation of DCs from peripheral tissues to lymph nodes (LNs).¹⁴ Naive CD4⁺ T cells circulating through LNs in search of their cognate antigen/MHCII ligands require additional costimulatory signals, delivered by mature DCs, to proliferate and survive as effector cells.¹⁵ In the absence of DC maturation, T cells proliferate to the presented antigen but either die or become anergic leading to antigen-specific tolerance.¹⁶ Some metals, including the commonly used vaccine adjuvant aluminum hydroxide, have adjuvant properties that boost CD4⁺ T-cell responses via effects on DCs.^{17, 18} Beryllium has also been shown to have adjuvant properties that drive lymphocyte expansion and Th1 cytokine production.^{19, 20, 21} Thus beryllium may operate not only as an antigenic stimulus but also as its own pathogenic adjuvant. We hypothesize that pulmonary exposure to beryllium leads to the release of DAMPs and activation of innate PRRs that promote pulmonary DC maturation and expansion of effector CD4⁺ T cells.

Here we show that pulmonary exposure to beryllium induces rapid release of IL-1α and DNA, DAMPs associated with necrotic cell death.²² IL-1α induces neutrophil infiltration, and neutrophils partially contribute to the release of DNA. Beryllium-induced release of DAMPs into the lung is associated with accumulation of mature migratory classical DCs (cDCs) in the lung-draining LNs (LDLNs) and enhanced priming of CD4⁺ T cells to a model antigen. The accumulation of cDCs in the LDLN following beryllium exposure is IL-1R independent. Although cDC migration is impaired in Toll-like receptor knockout (TLR9KO) mice, increased CD80 and CD86 expression on cDCs occurs, and the adjuvant effects of beryllium on CD4⁺ T cells are intact in these mice. cDC migration, expression of costimulatory molecules, and CD4⁺ T-cell priming are all impaired in myeloid differentiation primary response gene 88 knockout (MyD88KO) mice, suggesting that other MyD88-dependent receptors in concert with DNA/TLR9 have a role in the effects on cDCs. Together, these data show that pulmonary beryllium exposure leads to release of DNA and other DAMPs that drive MyD88-dependent maturation and migration of cDCs from the lung to the LDLNs, leading to enhanced priming of naive CD4⁺ T-cell responses.

RESULTS

Pulmonary exposure to beryllium induces cellular death, release of DNA and IL-1α, and recruitment of neutrophils into the lung

To determine whether IL-1 or DNA was released following exposure to beryllium in the lung, we performed intratracheal (i.t.) instillations of saline or beryllium hydroxide (Be(OH)₂) in wild-type (WT) C57Bl/6 (B6) mice and performed bronchoalveolar lavage (BAL) (Figure 1). Compared with untreated mice (time 0), IL-1α and extracellular DNA were increased in the BAL fluid (BALF) at 6 and 18 h after beryllium exposure (Figure 1a,b). This increase was associated with an increased percentage of cells that stained positive with an eFluor506-labeled fixable viability dye (FV-e506) 6 h after treatment with Be(OH)₂ compared with saline-treated controls (Figure 1c and see Supplementary Figure S1A online). This dye stains necrotic, necroptotic, and late apoptotic cells but not cells undergoing early apoptosis. Analysis of FV-e506⁻ cells for early apoptosis using annexin V indicated an increase in early apoptotic cells in beryllium-exposed mice (see Supplementary Figure S1B,C) and as previously reported.²³ However, release of IL-1α occurs following necrosis or necroptosis (a regulated form of necrosis) and not apoptosis.^{22, 24} Together, our data suggest that both apoptotic and necrotic/necroptotic cell death occur in the lung in response to beryllium, the latter contributing to the release of DAMPs.

Surprisingly, we did not detect IL-1β in the BALF of mice exposed to beryllium at these time points (data not shown). We analyzed live cells for the presence of alveolar macrophages (F4/80⁺ CD11c⁺ SiglecF⁺) and neutrophils (F4/80⁻ Ly6G⁺) in the BAL using flow cytometry (Figure 1d,e). Beryllium exposure resulted in a drop in the percentage of macrophages and an increase in the percentage of neutrophils in the BAL 6 and 18 h after instillation (Figure 1d,e). The total number of BAL neutrophils increased 6 and 18 h after beryllium exposure while the number of alveolar macrophages dropped 1 h after exposure and gradually recovered by 18 h (Figure 1d,e). Together, these data show that beryllium induces rapid release of DAMPs, including DNA and IL-1α, followed by recruitment of neutrophils into the lung.

Beryllium-induced recruitment of neutrophils is dependent on IL-1α

IL-1α and IL-1β bind to the IL-1R, which induces the release of chemokines and recruitment of neutrophils.^{22, 25} The release of biologically active IL-1β requires activation of caspase-1 by the inflammasome.^{26, 27} The Nlrp3 inflammasome has been shown to be activated in response to a number of particles, including silica, asbestos, and aluminum hydroxide.^{28, 29} In contrast, IL-1α is released along with DNA from necrotic cells during sterile inflammation (inflammation induced in the absence of microbial components) and does not require caspase-1.²² In accordance with this, IL-1α release was not impaired in beryllium-treated caspase-1KO mice (Figure 2a). Both forms of IL-1 can bind to and activate the IL-1R and drive neutrophil recruitment; however, release of IL-1α from necrotic cells drives neutrophil recruitment in the absence of inflammasome activation.²⁵ To determine whether the recruitment of neutrophils required IL-1R or caspase-1, we exposed WT, IL-1RKO, MyD88KO, and caspase-1KO mice to i.t. Be(OH)₂ and analyzed neutrophil recruitment in the lungs 18 h later. Neutrophil recruitment was significantly reduced in beryllium-exposed IL-1RKO and MyD88KO mice but was intact in similarly treated caspase-1KO mice (Figure 2b), confirming that IL-1α, and not IL-1β, released after beryllium exposure was critical for neutrophil recruitment to the lung.

IL-1α engages IL-1R in the lung and drives expression of the neutrophil chemokine, keratinocytes (KC).^{22, 30} We observed that KC was increased in the BALF of WT mice exposed to beryllium and was attenuated in beryllium-exposed IL-1RKO mice (Figure 2c). Thus our data suggest that release of IL-1α is required for neutrophil recruitment to the lung through induction of KC.

Neutrophils contribute but are not the only source of beryllium-induced release of extracellular DNA

In mice treated with aluminum hydroxide, cellular death leads to the accumulation of DNA having characteristics of neutrophil extracellular traps (NETs).³¹ To determine whether beryllium exposure leads to NETosis, we purified human neutrophils and incubated them with media or Be(OH)₂ for 4 h. Beryllium induced the release of extracellular DNA that colocalized with the neutrophil granule protein elastase, a situation that only occurs in neutrophils undergoing NETosis (Figure 2d). However, DNA was released into the BAL of beryllium-treated mice with similar kinetics as IL-1α, suggesting that neutrophils are not required for the release of DAMPs. To test this, we compared the levels of extracellular DNA in the BALF of beryllium-treated WT mice with those in beryllium-treated IL-1RKO mice, which do not recruit neutrophils to the lung. We observed a reduction in the total quantity of DNA in the BALF of treated IL-1RKO mice compared with WT control mice, but the levels of DNA remained increased above saline-treated control mice, suggesting that neutrophils contribute to the release of extracellular DNA but are not the sole source (Figure 2e). Thus other cells such as epithelial cells or macrophages contribute to DAMPs released following beryllium exposure.

Beryllium exposure drives migration of pulmonary DCs into the draining LN

Our data indicate that release of DAMPs occurs rapidly after exposure to beryllium in the lung. Innate PRRs and IL-1Rs engaged by DAMPs could impact DC function and the development and maintenance of beryllium-specific CD4⁺ T cells in CBD. To examine this, we determined whether beryllium exposure was associated with increased migration of DCs from the lung to the draining LNs. To track pulmonary DCs, we exposed mice i.t. to AF647-labeled ovalbumin (AF647-ova) in the absence or presence of beryllium and followed the arrival of AF647-ova⁺ DCs in the LDLNs. The percentage of cDCs (MHCII⁺CD11c⁺ cells) in the LDLNs was increased in mice treated with AF647-ova+beryllium compared with mice treated with AF647-ova alone (Figure 3a). The percentage of LDLN cDCs that were AF647-ova⁺ was also increased in beryllium-exposed mice (Figure 3b). As expected, AF-647-ova⁺ cDCs in the lung expressed high levels of MHCII, a phenotype characteristic of migratory cDCs³² (Figure 3c).

We next determined whether beryllium exposure altered costimulatory molecule expression on the surface of cDCs in the LDLNs. AF647-Ova⁺ cDCs that had migrated to the LDLN had increased levels of CD80 and CD86 on their surface compared with AF647-Ova⁺ cDCs from mice exposed to AF647-ova alone or to steady-state LDLN cDCs from untreated mice (Figure 3d). LDLN AF647-ova⁻ cDCs in mice treated with AF647-ova+beryllium also upregulated CD80 and CD86 (see Supplementary Figure S2), suggesting that beryllium exposure resulted in global maturation of cDCs in the LDLNs.

Migratory pulmonary cDCs consist of CD11b⁺ and CD103⁺ DC subsets.³³ CD103⁺ DCs are specialized to phagocytize apoptotic cells and cross present exogenous antigen on MHCI molecules; however, both subsets can present antigen to CD4⁺ T cells.³³ Analysis of CD11b⁺ and CD103⁺ cDC subsets (Figure 3e) showed an increased percentage of the AF647-ova⁺ cDCs in AF647-ova+beryllium-treated mice of the CD11b⁺ subset compared with mice treated with AF647-ova alone. However, the total numbers of both AF647-ova⁺ CD11b⁺ and CD103⁺ cDCs were increased in the LDLNs after beryllium exposure (Figure 3f).

Beryllium enhances the ability of DCs to prime CD4⁺ T cells in vivo

To determine whether the effects of beryllium on cDCs enhanced CD4⁺ T-cell priming, we compared CD4⁺ T-cell responses against a model antigen in the LDLNs of B6 mice in the presence or absence of beryllium. We used a well-characterized peptide–protein conjugate of the 3K peptide (FEAQKAKANKAVDGGGC) covalently linked to ova (3K-ova), because this antigen is presented by IA^b MHCII molecules in B6 mice, and we can track endogenous 3K-specific CD4⁺ T-cell responses using MHCII tetramers.^{17, 34, 35} WT B6 mice were exposed i.t. to 3K-ova in the absence or presence of Be(OH)₂, and the 3K-specific CD4⁺ T-cell response in the LDLNs was analyzed over time. Beryllium enhanced the magnitude of the primary CD4⁺ T-cell response, which peaked 14 days after instillation (Figure 4a). Upon secondary exposure to beryllium and 3K-ova, memory CD4⁺ T cells expanded to greater numbers compared with the primary response (Figure 4b). Thus beryllium-induced enhancement of cDC migration and activation is associated with adjuvant effects on CD4⁺ T cells.

Accumulation of pulmonary cDCs in LDLNs after beryllium exposure is IL-1R independent and reduced in TLR9KO mice

To determine whether IL-1R signaling is required for the effects of beryllium on cDC migration and activation, we exposed mice to AF647-ova in the presence or absence of Be(OH)₂ and compared the accumulation and activation of AF647-ova⁺ cDCs in the LDLNs of WT and IL-1RKO mice 48 h later. The accumulation of AF647-ova⁺ CD11b⁺ and CD103⁺ cDCs was enhanced following beryllium exposure and was similar in LDLNs of WT and IL-1RKO mice (Figure 5a). CD80 and CD86 were similarly upregulated on AF647-Ova⁺ cDCs from beryllium-exposed WT and IL-1RKO mice (Figure 5b). Thus IL-1R signaling is not required for the observed effects of beryllium on pulmonary cDCs.

TLRs are PRRs that can recognize certain DAMPs and promote activation and migration of cDCs to LNs. Self-DNA stimulates signaling via TLR9 when delivered to endosomal compartments in DCs.³⁶ To determine whether TLR9 was involved in the effects of DNA on cDC migration and activation, we treated WT or TLR9KO mice with AF647-ova+beryllium and followed the accumulation of AF647-Ova⁺ cDCs in the LDLNs 48 h later. The total numbers of cDCs accumulating in the LDLNs of TLR9KO mice were reduced compared with WT mice (Figure 5c). However, AF647-Ova⁺ cDCs in the LDLNs of beryllium-exposed WT and TLR9KO mice expressed similar levels of CD80 and CD86 (Figure 5d). Thus TLR9 may recognize DNA and contribute to the observed effects on DC migration, but other pathways contribute to optimal upregulation of costimulatory molecules on cDCs in the draining LN.

Adjuvant effects of beryllium on CD4⁺ T-cell priming are independent of IL-1, TLR4, TLR7, TLR9, and the inflammasome

Although we did not observe significant differences in the accumulation of cDCs in the draining LNs between WT and IL-1RKO mice (Figure 6a), it was possible that other members of the IL-1 family could contribute to the adjuvant effects of beryllium on CD4⁺ T cells or that IL-1 could directly impact CD4⁺ T-cell priming. Although we did not detect endotoxin in our Be(OH)₂ stock, we also wanted to exclude the possibility that low-level endotoxin contamination was having a role in our observations. Thus we exposed WT, IL-1RKO, caspase-1KO, and TLR4KO mice to 3K-ova in the presence of beryllium and analyzed the number of 3K-specific CD4⁺ T cells in the LDLNs 14 days after exposure. We found similar numbers of 3K-specific CD4⁺ T cells in all the groups of beryllium-treated mice (Figure 6a), suggesting that the adjuvant effects of beryllium on CD4⁺ T-cell priming are independent of IL-1R, the inflammasome, inflammasome-dependent cytokines of the IL-1 family, and TLR4.

To determine whether TLR9 or as a control, TLR2, had a role in these adjuvant effects, we treated WT, TLR9KO, and TLR2KO mice with 3K-ova in the presence or absence of beryllium. Although accumulation of cDCs in the LDLNs at 48 h was reduced in TLR9KO mice exposed to beryllium (Figure 5c), the adjuvant effects of beryllium on CD4⁺ T-cell priming were TLR9 independent (Figure 6b). TLR7 can respond to nucleic acids; however, the adjuvant effects of beryllium on CD4⁺ T-cell priming were intact in TLR7KO mice (Figure 6c). Collectively, these data suggest that, while DNA/TLR9 enhance cDC migration, signaling via other PRRs are sufficient to enhance costimulatory signals on cDCs and the adjuvant effects of beryllium on CD4⁺ T-cell priming.

The adjuvant effects of beryllium are dependent upon MyD88 signaling pathways

Our data suggest that redundant PRRs are involved in beryllium-induced DC migration and CD4⁺ T-cell priming. MyD88 is a signaling adaptor that is downstream of multiple innate PRRs that recognize DAMPs.³⁷ Therefore, it is possible that disruption of multiple pathways that rely on MyD88 would eliminate the adjuvant effects of beryllium exposure. To globally test the role of MyD88-dependent receptors on DC migration, we treated WT and MyD88KO mice i.t. with AF647-ova±Be(OH)₂ and analyzed cDCs in the LDLNs at 24 and 48 h. We observed a reduction in the accumulation of AF647-ova⁺ CD11b⁺ and CD103⁺ cDCs in the LDLN after exposure in MyD88KO mice compared with WT mice (Figure 7a). We also observed that upregulation of CD80 and CD86 on cDCs was impaired in beryllium-exposed MyD88KO mice (Figure 7b).

To determine whether these effects were associated with a reduction in the adjuvant effects of beryllium on CD4⁺ T-cell priming, we treated WT and MyD88KO mice with 3K-ova and beryllium and analyzed the expansion of 3K-specific CD4⁺ T cells in the LDLN 12 days later. We observed a significant reduction in the total number of 3K-specific CD4⁺ T cells in MyD88KO mice compared with WT mice (Figure 7c). Thus our findings show that the effects of beryllium exposure on DC function and the adjuvant effects of beryllium on CD4⁺ T cells involve PRRs that signal via MyD88-dependent pathways.

DISCUSSION

CBD is a granulomatous lung disease that is characterized by the accumulation of beryllium-responsive CD4⁺ T cells in the lung. In the past two decades, much has been learned about beryllium as a disease-inducing MHCII-restricted antigen; however, the development of CBD involves complex interactions between the innate and adaptive arms of the immune system. In this regard, beryllium possesses adjuvant properties that may contribute to CBD pathogenesis; however, the mechanism behind these adjuvant effects is unknown.^{19, 20} DCs are critical antigen-presenting cells that determine whether T-cell receptor engagement results in a pathogenic or tolerogenic response. However, nothing is known about how beryllium impacts DCs in the target organ. Although beryllium-induced apoptosis has been described,²³ apoptosis is not associated with the release of DAMPs or initiation of inflammation. In addition to apoptosis, our data show that beryllium exposure in the lung induces necrotic cell death and the release of DAMPs, including DNA and IL-1α, that drive inflammation. These effects are associated with MyD88-dependent migration of mature cDCs to LDLNs that enhance priming of naive CD4⁺ T cells, an effect that is IL-1R independent.

IL-1β has been shown to be important in inducing release of chemokines that drive neutrophils to infiltrate the lung in response to other sterile environmental agents, including silica particles and asbestos.^{28, 29} However, these studies suggest a critical role for Nlrp3, which becomes activated following phagocytosis of large particles that disrupt lysosomal membranes and induce cellular stress. Activated Nlrp3 promotes assembly of the inflammasome, activation of caspase-1, and release of biologically active IL-1β. In contrast, we did not detect IL-1β in the alveolar space after exposure to beryllium, and neutrophil recruitment was IL-1R dependent but independent of caspase-1, an enzyme required for the release of biologically active IL-1β. These data suggest that IL-1α has an essential role in driving neutrophil recruitment after beryllium exposure and are consistent with studies showing a critical role for IL-1α in the recruitment of neutrophils in response to necrotic/necroptotic but not apoptotic cells.^{24, 25} It is possible that beryllium induces more necrotic cell death and IL-1α release while other particles induce inflammasome activation following phagocytosis. Thus the roles of IL-1α and IL-1β may be related to differences in toxicity between different types of sterile particles. Other studies have shown that IL-1α and IL-1β are released at different times and drive recruitment of neutrophils and monocytes, respectively.³⁸ Thus, it is possible that IL-1β may be induced later in response to beryllium.

Our data show that, after arrival in the lung, neutrophils contribute to DNA release in vivo, perhaps through NETosis, but are not required for release of DNA. Thus, other cells, including macrophages or epithelial cells, may contribute to the initial DNA release. Our analysis of ex vivo DNA does not discriminate between NETs and extracellular DNA; however, macrophages and epithelial cells have not been described to undergo NETosis. Thus the DNA detected in the absence of neutrophils likely reflects DNA released by necrosis. Neutrophils have been shown to impact pulmonary DC function during infection.³⁹ However, in IL-1RKO mice, which do not recruit neutrophils to the lung after beryllium exposure, cDC migration to LDLNs was intact, suggesting that the effects of beryllium exposure on cDCs are not dependent upon neutrophils or NETosis but may still be impacted by extracellular DNA.

Introduction of apoptotic cells into the lung has been shown to preferentially induce the migration of CD103⁺ cDCs into LDLNs.³³ In contrast, beryllium exposure increases the migration of both cDC subsets. cDCs from beryllium-exposed mice exhibited upregulated levels of the costimulatory molecules CD80 and CD86 and more effectively primed CD4⁺ T cells to bystander antigen. These effects were not reduced in IL-1RKO or TLR4KO mice. TLR2, TLR9, and TLR7 have been implicated in innate responses to other sterile substances in the lung.⁴⁰ We found no difference in the adjuvant effects of beryllium on CD4⁺ T-cell priming in WT, TLR2, TLR7, or TLR9KO mice. We did observe a reduction in beryllium-induced migration of cDCs in TLR9KO mice; however, cDCs in the draining LN of these mice were highly activated, suggesting that other pathways are involved. It is possible that the high levels of CD80 and CD86 on these cDCs promote enhanced priming of CD4⁺ T cells, even in the presence of fewer cDCs in the TLR9KO mice. Thus, despite evidence that TLR9 is engaged by DNA, our data suggest that multiple PRRs have a role in optimal cDC function. Together, our data suggest that, in addition to TLR9, other PRRs are engaged that activate MyD88 signaling and promote cDC activation and priming of CD4⁺ T cells. Our findings support a model where DAMPs released in the lung following beryllium exposure impact DC expression of CD80 and CD86 and beryllium does not directly upregulate these costimulatory molecules, consistent with recent studies of human DCs.⁴¹

Beryllium and aluminum are both metals with adjuvant properties.⁴² Aluminum hydroxide is a widely used vaccine adjuvant that induces the release of DNA and activation of the inflammasome.^{43, 44} In contrast to pulmonary beryllium exposure, aluminum hydroxide injected into the muscle does not enhance DC migration into peripheral LNs, perhaps due to differences in the types of DCs that are mobilized and/or the ability of antigen to be moved without DC help through the lymphatics to the popliteal LN by skeletal muscle contractions.^{17, 45, 46} In contrast to beryllium that induces Th1 responses and neutrophil recruitment, aluminum hydroxide induces a Th2-type response and eosinophilia.⁴⁷ The adjuvant function of intraperitoneal- or intramuscular-administered aluminum hydroxide on humoral responses is MyD88 independent.⁴⁸ It is possible that the differences between aluminum and beryllium are due to different anatomical locations studied, differences in DC subsets and the PRRs expressed, and/or differences in metal-induced toxicity and DAMPs released at the exposure site.

In the context of CBD, the development of a strong beryllium-specific effector CD4⁺ T-cell response is necessary for the generation of granulomatous inflammation in the lung.⁸ This is limited to individuals expressing particular MHCII molecules capable of presenting beryllium/peptide complexes to CD4⁺ T cells. We hypothesize that, in individuals with this genetic predisposition, beryllium-induced activation of MyD88-dependent pathways may promote the initiation of a beryllium-specific immune response in the lung via effects on DCs and may further impact the development of beryllium-specific effector Th1 and regulatory T cells following beryllium exposure. In addition, these adjuvant properties of beryllium may have a dose-sparing effect, effectively promoting beryllium-induced sensitization in response to low exposures. Although our study is most relevant to understanding the effects of beryllium on the sensitization phase, these pathways may also operate at later stages of disease.

The activation of inflammation and effects on DCs were transient in our study, showing a wave of DC migration into the LDLN that was reduced after 72 h. This may be due to fluorescent dye breakdown or to a transient release of DAMPs occurring within a short time after exposure. Further studies are needed to understand how these pathways impact the effects during chronic disease on both DCs and T cells. In reality, workers are exposed to beryllium over long periods of time, and while it is known that beryllium persists long after exposure, the initial events following exposure to DCs are likely most important in driving proliferation and differentiation of naive CD4⁺ T cells to beryllium (i.e., sensitization). Once primed, effector and memory CD4⁺ T cells rely less heavily upon costimulation, and antigen-presenting cells in vivo are likely not restricted to DCs alone but include B cells and macrophages. Analysis of the role of these different antigen-presenting cells in CBD and whether DAMP release impacts these cells and granuloma development at later stages of disease remain important unanswered questions.

Taken together, we show for the first time that beryllium drives IL-1-dependent neutrophil accumulation and IL-1-independent migration of activated pulmonary cDCs to LDLNs. Recognition of DNA via TLR9 and engagement of another unknown MyD88-dependent PRR leads to the accumulation of activated DCs in the LDLN and enhancement of CD4⁺ T-cell priming. In addition to its role as a pathogenic antigen in CBD, our data show that beryllium can potently stimulate the innate immune system and enhance DC function, effectively functioning as an adjuvant in CBD.

METHODS

Mice. C57Bl/6 WT, MyD88KO, Caspase-1KO, IL-1RKO, TLR2KO, TLR7KO, and TLR4KO mice were purchased from Jackson Laboratories (Bar Harbor, ME). TLR9KO mice were kindly provided by Dr Jillian Poole (University of Nebraska Medical Center) with permission from Shizuo Akira. Mice were housed and bred at the University of Colorado Biological Resource Center and were used at 6–12 weeks of age. All experiments were approved by the Institutional Animal Care and Use Committee of the University of Colorado Denver, in accordance with the NIH guidelines.

Reagents. Be(OH)₂ was generated as described.²⁰ AlexaFluor647 was conjugated to low endotoxin ova (Hyglos, Munich, Germany) using the Pierce AF647 Labeling Kit according to the manufacturer’s instructions. 3K-ova was generated using the Imject Maleimide Activated Ova Kit from Thermo Fisher Scientific (Rockford, IL) and a cysteine linked 3K peptide.⁴⁹

Treatments. Mice were anesthetized with isoflurane, and the vocal chords were visualized with a laryngoscope (Penn-Century, Wyndmoor, PA) followed by i.t. delivery of 50 μl volume 0.9% sterile saline or phosphate-buffered saline (PBS) with or without sonicated 200 μg Be(OH)₂. In some experiments, 20 μg AF647-ova or 10 μg 3K-ova were mixed with beryllium.

Early innate responses to beryllium in the lung. Mice were killed after exposure to saline or Be(OH)₂, and BAL was performed with 1.5 ml of sterile PBS. Cells were separated from the BALF using centrifugation, and viable cells were enumerated using trypan blue. Beryllium crystals have a crystalline morphology that can be clearly distinguished from viable cells. For IL-1 analysis, BALF were concentrated fivefold using Amicon 3-kDa filters from EMD Millipore (Billerica, MA). IL-1α and IL-1β were quantified using Ready Set Go enzyme-linked immunosorbent assay (eBioscience, San Diego, CA).

BAL cells were incubated with FV-eFluor 506 for 30 min on ice (eBioscience), washed, and incubated with Fc Block and monoclonal antibodies directed against Ly6G, Siglec F, F4/80, and Ly6C. Live and dead cells were gated based on unstained controls (see Supplementary Figure S1A). Live cells were analyzed for the presence of neutrophils and macrophages and, for apoptotic cells, using the FITC Annexin V Kit (eBioscience). Data were acquired on a LSRII SORP flow cytometer using FACS Diva Software (BD Biosciences) and analyzed using FlowJo software (FlowJo, LLC, Ashland, OR).

Quantification of extracellular DNA following in vivo exposure of mice to beryllium. BALF samples were diluted with PBS and added to a 96-well black microplate (Greiner Bio-One, Monroe, NC). Mouse genomic DNA (Promega, Madison, WI) was used to generate a standard curve. The samples were incubated with a solution of 5 μM Sytox Orange (Life Technologies, Grand Island, NY) in PBS for 10 min at room temperature. The plate was analyzed using a Victor³ 1420 multilabel plate reader with the Wallac 1420 Workstation software (Perkin Elmer, Waltham, MA) with a 544-nm excitation filter and a 600-nm emission filter.

Neutrophil NET release. Neutrophils from healthy human volunteers were isolated as described (see details in Supplementary Methods),⁵⁰ analyzed for purity by flow cytometry, and stained using fluorescently labeled anti-CD49d and -CD66 monoclonal antibodies (eBioscience). Cells were resuspended in complete RPMI media (cRPMI) consisting of RPMI 1640 tissue culture medium (Life Technologies) supplemented with 10% heat-inactivated fetal bovine serum (HyClone, Logan, UT), 10 mM HEPES, 1 mM sodium pyruvate, 100 U ml⁻¹ penicillin, and 100 μg ml⁻¹ streptomycin (all from Life Technologies). Cells were incubated in a 48-well plate for 4 h in media alone or with 1.2 mg Be(OH)₂, washed with PBS, and fixed for 15 min in paraformaldehyde at room temperature. Cytospins of ∼100,000 cells per slide were prepared and stained with phycoerythrin-labeled anti-human elastase (Calbiochem, San Diego, CA) and Hoesht to visualize DNA. Cells were washed and coverslipped with VectaMount medium (Vector Labs, Burlingame, CA). Slides were analyzed on an Olympus BX-41 fluorescent microscope (CytoViva, Auburn, AL), and images were taken using a Dage-MTI Excel digital camera XLMCT (Michigan City, IN).

Effects of beryllium on pulmonary DC migration and expression of costimulatory molecules. LDLN were harvested, and single cell suspensions were generated as described (see Supplementary Methods).¹⁷ Cells were incubated with FcBlock and stained with fluorescently conjugated monoclonal antibodies to CD11c, IA^b, CD11b, CD80, CD103, and CD86.

Effects of beryllium on CD4⁺ T-cell priming. At the indicated times after instillation, lungs were flushed of blood by placing an incision in the left atrium of the heart and passing 10 ml PBS through the lungs with a syringe, and a 25-gauge needle was introduced into the left ventricle of the heart. Lungs and LDLNs were harvested into cRPMI media and processed into single-cell suspensions as described (details in Supplementary Methods).^{8, 17} Viable cells were enumerated using Trypan blue exclusion and stained with phycoerythrin-labeled 3K/IA^b tetramers at 37 °C for 2 h. Antibodies for CD4, B220, MHC II, F4/80, CD44, and CD8 were added for 20 min at 4 °C. 3K/IA^b tetramer–positive cells were defined after gating on live CD4⁺CD44^hi cells that were CD8, B220, F4/80, and MHCII negative.

Statistical analysis. A one-way analysis of variance and unpaired t-test were used to determine significance of differences between groups (Prism 4, GraphPad Software, La Jolla, CA). A P-value <0.05 was considered statistically significant.

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Acknowledgements

This work is supported by the following grants: American Thoracic Society Unrestricted Grant (to ASM), and NIH grants HL111760 and ES11810 (to APF). We thank Philippa Marrack, Michael Falta, Amanda Goodluck, Nicole Desch, Claudia Jakubzick, and Peter Henson for technical feedback on this project. We thank Allison Martin for technical assistance.

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Department of Medicine, University of Colorado Anschutz Medical Campus, Aurora, Colorado, USA
A S McKee, D G Mack & A P Fontenot
Department of Immunology, University of Colorado Anschutz Medical Campus, Aurora, Colorado, USA
F Crawford & A P Fontenot
Department of Biomedical Research, National Jewish Health, Denver, Colorado, USA
F Crawford

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A S McKee
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D G Mack
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F Crawford
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A P Fontenot
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McKee, A., Mack, D., Crawford, F. et al. MyD88 dependence of beryllium-induced dendritic cell trafficking and CD4⁺ T-cell priming. Mucosal Immunol 8, 1237–1247 (2015). https://doi.org/10.1038/mi.2015.14

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Received: 02 October 2014
Accepted: 12 January 2015
Published: 11 March 2015
Issue Date: November 2015
DOI: https://doi.org/10.1038/mi.2015.14

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