Correction to: Leukemia (2012) 26, 1338–1347; doi:10.1038/leu.2011.331

Since the publication of this article, the authors have noticed an error contained within panel f of Figure 1. The correct figure is shown here.

Figure 1
figure 1

Interaction studies of PU.1 and the AML-associated fusion proteins. (a) ‘Mammalian two-hybrid’ (MTH) analysis. MTH assays were performed in 293 cells transfected with the PU.1 ‘bait’ plasmid (GAL4-PU.1) and the respective ‘prey’ plasmid (VP16 empty control, VP16-RARa, VP16-PLZF, VP16-PLZF/RARα, VP16-PML and VP16-PML/RARα). The results are expressed as relative light units (RLU), representing the activation of the (GAL)4-luciferase reporter normalized to the internal renilla control. Data are presented as the mean±s.d. of three independent experiments, each performed in triplicates. *P<0.05, ***P<0.001 and NS=not significant represent the significance with respect to the VP16 control. Statistical analysis was performed by Student’s t-test. (b) Western blot analysis of the expressed fusions. Proteins were separated by SDS-PAGE and transferred onto nitrocellulose membranes. The membranes were probed with anti-VP16 or anti-PU.1 antibodies as indicated. (c) (Upper panel) co-immunoprecipitation (IP) of Phoenix cells expressing X-RARα and PU.1 as indicated. IP was performed with the anti-PU.1 antibody and western blot analysis was performed using the indicated antibodies. Input, 5% of the protein amount used for the co-IP reaction. (Lower panel) IP was performed with the anti-PU.1 antibody under ChIP conditions, and western blot analysis was performed using the indicated antibodies. Control, mock-transfected U937 cells. Input, 25% of the protein amount used for the co-IP reaction. (d–f) Influence of the expression of PML/RARα and PLZF/RARα on the localization of PU.1. Control, wild-type cells or cells transfected with empty plasmids. Cells were stained with Hoechst 33342 and the indicated antibodies. Anti-SNAP, polyclonal rabbit antibody conjugated to red fluorochrome (anti-SNAP). Anti-PU.1, polyclonal rabbit antibody conjugated to red fluorochrome (anti-PU.1). Anti-PML and anti-PLZF, monoclonal mouse antibodies conjugated to green fluorochrome (anti-PML and anti-PLZF). Co-localization images were obtained by electronic overlapping of the images (merge). Co-localization of the red and green fluorochromes yields a yellow color. (d) NIH-3T3 cells were transfected as indicated. (e, f) U937 cells harboring the indicated fusion protein or empty control.

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The authors would like to apologize for any inconvenience this may have caused.