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  • Clinical Techniques
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Middle ear sample collection in the chinchilla

Abstract

Use of the chinchilla as a model of middle ear infection in auditory research necessitates accessing the middle ear to collect samples or inoculate bacteria. This column describes a simple technique for middle ear access. The procedure is also useful for investigating and treating middle ear infections in chinchillas.

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Figure 1: An anesthetized chinchilla in ventral recumbency with tympanic bulla (arrow) visible at the site devoid of fur, rostral and medial to the base of the pinna.
Figure 2: An anesthetized chinchilla in ventral recumbency with a sterile fenestrated drape over the aseptically prepared tympanic bulla.
Figure 3: A sterile piece of butterfly infusion set tubing is inserted into the opening created by the 16-gauge needle in the dorsal aspect of the tympanic bulla.
Figure 4: A fenestration has been created in the dorsal aspect of the tympanic bulla of a chinchilla with a 16-gauge needle.

References

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Brown, C. Middle ear sample collection in the chinchilla. Lab Anim 36, 22–23 (2007). https://doi.org/10.1038/laban1007-22

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  • DOI: https://doi.org/10.1038/laban1007-22

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