Abstract
Discriminating between phenotypically indistinguishable heterozygous and wild-type mice presents a difficult situation for researchers attempting to find suitable breeding pairs. The authors describe a fast, reliable, nonradioactive method that combines PCR with capillary electrophoresis to determine genotypical differences among heterozygous (+/op), homozygous (op/op), and wild-type (+/+) mice.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
We are sorry, but there is no personal subscription option available for your country.
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
Schonlau, F., Schlesiger, C., Ehrehen, J., Grabbe, S., Sorg, C. & Sunderkotter, C. Monocyte and macrophage functions in M-CSF–deficient op/op mice during experimental leishmaniasis. J. Leukoc. Biol. 73(5), 564–573 (2003).
Doherty, T.M. et al. Rationale for the role of osteoclast-like cells in arterial calcification. FASEB J. 16(6), 577–582 (2002).
Kaneko, H., Iizuka, K., Morohashi, T., Kokai, Y. & Fujimoto, J. Non-phenotypic detection of osteopetrotic (op/op) mutation by using PCR-SSCP analysis. Lab. Anim. 29(4), 442–446 (1995).
Yoshida, H. et al. The murine mutation osteopetrosis is in the coding region of the macrophage colony stimulating factor gene. Nature 345(6274), 442–443 (1990).
Gerstner, A., DeFord, J. & Papaconstantinou, J. Comparison of two PCR-based methods and automated DNA sequencing for Prop1 genotyping in Ames dwarf mice. Mutat. Res. 528(1–2), 37–44 (2003).
Acknowledgements
The authors thank Luisita Egger (Institute of Legal Medicine, Zentralkrankenhaus St. Jürgen Strasse, Bremen, Germany) and Stephanie Fischer (Working Group for Experimental and Clinical Arteriogenesis at the Department for Internal Medicine III, Albert-Ludwigs University of Freiburg, Germany) for their superb technical assistance. A grant from the Volkswagen Foundation supported this study.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Techel, D., Bergmann, C., Buschmann, I. et al. Discrimination of Heterozygous from Wild-Type Mice Bearing a Mutation in the Macrophage–Colony-Stimulating Factor Gene Using Capillary Electrophoresis. Lab Anim 33, 33–35 (2004). https://doi.org/10.1038/laban0904-33
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/laban0904-33