Abstract
Intravenous vascular access is technically challenging in the adult mouse and even more challenging in neonatal mice. The authors describe the technique of retro-orbital injection of the venous sinus in the adult and neonatal mouse. This technique is a useful alternative to tail vein injection for the administration of non-tumorigenic compounds. The authors report that they have routinely used this technique in the adult mouse to administer volumes up to 150 μl without incident. Administration of retro-orbital injections is more challenging in neonatal mice but can reliably deliver volumes up to 10 μl.
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Acknowledgements
We thank Brenda Klaunberg and Danielle Donahue from the NIH Mouse Imaging Facility and Darryl Leja from the NHGRI Intramural Publication Support Office for their assistance with this project. This work was carried out in partial fulfillment of the requirements for a PhD degree for T.Y. (Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel). This research was supported in part by the Intramural Research Program of the NHGRI, the National Institutes of Health.
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Yardeni, T., Eckhaus, M., Morris, H. et al. Retro-orbital injections in mice. Lab Anim 40, 155–160 (2011). https://doi.org/10.1038/laban0511-155
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DOI: https://doi.org/10.1038/laban0511-155
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