Using fecal DNA for non-invasive genotyping

Transgenic animals can be identified by genotyping a sample of their genomic DNA from tissues such as the tail, ear or toe, but biopsy of these tissues may cause pain and distress. Stool is a reliable, non-invasive source of DNA for genotyping. Existing methods for extracting fecal DNA may be costly, however, or may involve the use of toxic organic solvents. Furthermore, feces contain an abundance of PCR inhibitors that may hinder DNA amplification when they are co-purified with fecal DNA. To encourage researchers to consider fecal DNA genotyping as an alternative to tail DNA genotyping for transgenic animals, Zhidong Chen and colleagues developed AquaStool, a safe, cost-effective reagent that may be used to extract and remove PCR inhibitors from fecal DNA for genotyping. The authors show that animals can be identified with high specificity and sensitivity by genotyping fecal DNA samples extracted using AquaStool. Use of this non-invasive method for genotyping transgenic animals may improve animal welfare.

See page 102

Zebrafish anatomy reviewed

Atlas of Zebrafish Development provides a detailed anatomical reference for zebrafish, spanning its life history from embryo to adult. Michael Stoskopf reviews the Atlas with the research community in mind.

See page 101

Developing international animal-based research programs

Bryan Ogden of SingHealth Experimental Medicine Centre in Singapore describes what inspired him to work with nonhuman primates and the challenges of developing animal-based research programs in Southeast Asia.

See page 108