Abstract
The authors present a technique for establishing a virtually stress-free dual-catheter system in rats, allowing researchers to conduct extended, complex experimental procedures involving the simultaneous infusion of multiple compounds and frequent collection of blood samples.
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References
Nachtman, R.G., Driscoll, T.B., Gibson, L.A. & Johnson, P.C. Jr. Commercial over-the-needle catheters for intravenous injections and blood sampling in rats. Lab. Anim. Sci. 38, 629–630 (1988).
Kraegen, E.W., James, D.E., Bennett, S.P. & Chisholm, D.J. In vivo insulin sensitivity in the rat determined by euglycemic clamp. Am. J. Physiol. 245, E1–7 (1983).
el-Shahawy, M.A. & Khilnani, H. Carotid-jugular arteriovenous fistula: a complication of temporary hemodialysis catheter. Am. J. Nephrol. 15, 332–336 (1995).
Guo, Z. & Jensen, M.D. Blood glycerol is an important precursor for intramuscular triacylglycerol synthesis. J. Biol. Chem. 274, 23702–23706 (1999).
Dworkin, B.R., Filewich, R.J., Da Costa, J., Eissenberg, E. & Miller, N.E. A chronic arterial catheter and low compliance system for recording blood pressure and heart rate from the rat. Am. J. Physiol. 239, H137–141 (1980).
Golden, S., Chenoweth, M., Dunn, A., Okajima, F. & Katz, J. Metabolism of tritium- and 14C-labeled alanine in rats. Am. J. Physiol. 241, E121–128 (1981).
Moslen, M.T., Kanz, M.F., Bhatia, J. & Catarau, E.M. Two cannula method for parenteral infusion and serial blood sampling in the freely moving rat. JPEN J. Parenter. Enterol. Nutr. 12, 633–637 (1988).
Furuhama, K. & Onodera, T. A simple technique for repeated blood collection from the tail vein of the rat. J. Toxicol. Sci. 8, 161–163 (1983).
Frank, P., Schoenhard, G.L. & Burton, E. A method for rapid and frequent blood collection from the rat tail vein. J. Pharmacol. Methods 26, 233–238 (1991).
Fejes-Toth, G., Naray-Fejes-Toth, A., Roage, D. & Frolich, J.C. Chronic arterial and venous catheterization of conscious, unrestrained rats. Hypertension 6, 926–930 (1984).
Hagmuller, K., Liebmann, P., Porta, S. & Rinner, I. A tail-artery cannulation method for the study of blood parameters in freely moving rats. J. Pharmacol. Toxicol. Methods 28, 79–83 (1992).
Fluttert, M., Dalm, S. & Oitzl, M.S. A refined method for sequential blood sampling by tail incision in rats. Lab. Anim. (NY) 34, 372–378 (2000).
Kim, J.K., Wi, J.K. & Youn, J.H. Plasma free fatty acids decrease insulin-stimulated skeletal muscle glucose uptake by suppressing glycolysis in conscious rats. Diabetes 45, 446–453 (1996).
Sacca, L., Toffolo, G. & Cobelli, C. V-A and A-V modes in whole body and regional kinetics: domain of validity from a physiological model. Am. J. Physiol. 263, E597–606 (1992).
Acknowledgements
The work was supported by the National Institute of Diabetes and Digestive and Kidney Diseases Grant DK 60013 and by a Pilot and Feasibility Program Project award from the Minnesota Obesity Center.
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Guo, Z., Zhou, L. Dual Tail Catheters for Infusion and Sampling in Rats as an Efficient Platform for Metabolic Experiments. Lab Anim 32, 45–48 (2003). https://doi.org/10.1038/laban0203-45
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DOI: https://doi.org/10.1038/laban0203-45
