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  • Original Article
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Usefulness of direct fluorescent in buffy coat in the diagnosis of Candida sepsis in neonates

Journal of Perinatology volume 36, pages 874–877 (2016)Cite this article

Subjects

A Corrigendum to this article was published on 30 September 2016

Abstract

Objective:

The objective of this study is to assess the clinical utility of direct fluorescent assay in buffy coat in the diagnosis of Candida sepsis (CS) in neonates.

Study design:

A cross-sectional study was conducted in a Neonatal Intensive Care Unit and 22 neonates with suspected CS were enrolled. Fungus isolation from blood cultures and direct fluorescent tests in buffy coat were performed and validity parameters were estimated.

Results:

Candida was isolated in 13/22 (59%) blood cultures. The direct fluorescent test was positive in 12/13 and 1/9 cases with positive and negative blood culture as corresponding. Estimated sensitivity, specificity, positive predictive value, negative predictive value, positive likehood ratio and negative likehood ratio were 92%, 89%, 92%, 89%, 8.31 and 0.09, respectively.

Conclusion:

The direct fluorescent assay in buffy coat might be useful to support early and accurate diagnosis of CS in neonates.

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Acknowledgements

This work was self-financed by the research group.

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Authors and Affiliations

  1. Jefatura de Servicios de Prestaciones Médicas, Instituto Mexicano del Seguro Social, Colima, Colima, México

    M A Higareda-Almaraz & E Higareda-Almaraz

  2. Departamento de Pediatría, Unidad Médica de Alta Especialidad, Centro Médico Nacional de Occidente, Instituto Mexicano del Seguro Social, Guadalajara, Jalisco, México

    H Loza-Barajas & J G Maldonado-González

  3. Jefatura de Servicios de Prestaciones Médicas, Instituto Mexicano del Seguro Social, Tepic, Nayarit, México

    V Benítez-Godínez

  4. Unidad de Medicina Familiar No. 19, Instituto Mexicano del Seguro Social, Colima, Colima, México

    E Murillo-Zamora

Authors
  1. M A Higareda-Almaraz
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  2. H Loza-Barajas
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Corresponding author

Correspondence to E Murillo-Zamora.

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Higareda-Almaraz, M., Loza-Barajas, H., Maldonado-González, J. et al. Usefulness of direct fluorescent in buffy coat in the diagnosis of Candida sepsis in neonates. J Perinatol 36, 874–877 (2016). https://doi.org/10.1038/jp.2016.96

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  • DOI: https://doi.org/10.1038/jp.2016.96

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