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Usefulness of serum lipid peroxide as a diagnostic test for hypoxic ischemic encephalopathy in the full-term neonate

Journal of Perinatology volume 33, pages 15–20 (2013)Cite this article

Subjects

Abstract

Objective:

To evaluate the usefulness of serum lipid peroxide (LPO) for hypoxic ischemic encephalopathy (HIE) in full-term neonates.

Study Design:

Diagnostic test evaluation forming three groups: (1) healthy full-term neonates (n=59), (2) at-risk full-term neonates without HIE (n=57) and (3) at-risk full-term neonates with HIE (n=57). HIE diagnosis was made using the Finer clinical classification at 48 h after birth. Serum LPO was taken at 4 h after birth and determined by spectrophotometry.

Result:

One hundred seventy-three full-term neonates were studied. Fifty-one of the at-risk full-term neonates with HIE (51/57) had high serum LPO and two of the at-risk full-term neonates without HIE (2/57) (P<0.001). Serum LPO level had 89% sensitivity, 96% specificity, 96% positive predictive value, 90% negative predictive value, 24 positive probability ratio, 0.11 negative probability ratio and 92% diagnostic usefulness.

Conclusion:

Serum LPO level could be a useful test for early diagnosis of HIE in full-term neonates.

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References

  1. Vannucci RC, Perlman JM . Interventions for perinatal hypoxic-ischemic encephalopathy. Pediatrics 1997; 100 (6): 1004–1014.

    Article  CAS  Google Scholar 

  2. Graham EM, Ruis KA, Hartman AL, Northington FJ, Fox HE . A systematic review of the role of intrapartum hypoxia-ischemia in the causation of neonatal encephalopathy. Am J Obstet Gynecol 2008; 199 (6): 587–595.

    Article  CAS  Google Scholar 

  3. Locatelli A, Incerti M, Ghidini A, Greco M, Villa E, Paterlini G . Factors associated with umbilical artery acidemia in term infants with low Apgar scores at 5 min. Eur J Obstet Gynecol Reprod Biol 2008; 139 (2): 146–150.

    Article  Google Scholar 

  4. Rosenberg AA, Jomes Jr M, Traystman RJ . Response of cerebral blood flow to changes in PCO2 in fetal, newborn, and adult sheep. Am J Physiol 1982; 242: 862–866.

    Google Scholar 

  5. De Boer J, Klein HC, Postema F . Rat striatal cation shifts reflecting hypoxic-ischemic damage can be predicted by online impedance measurements. Stroke 1989; 20: 1377–1382.

    Article  CAS  Google Scholar 

  6. Kellmer I, Hagberg H . Perinatal brain damage, excitatory amino acids and oxygen derived free radicals. In: Van Geijin HP, Copray FJ (eds). A Critical Appraisal of Fetal Surveillance. Elsevier Science: Amsterdam, 1994, pp 604–614.

    Google Scholar 

  7. Kattwinkel J . Manual de Reanimación Neonatal, 5th ed. Academia Americana de Pediatría y Asociación Americana del Corazón, Intersistemas editores, Mexico, 2006.

    Google Scholar 

  8. Poland RL, Feeman RK . Relations Between Perinatal Factors and Neurologic Outcome. Guidelines for Perinatal Care, 3rd ed. American Academy of Pediatrics. College of Obstetricians and Gynecologists, ELK ed., Grove Village, 1992, 221–224.

    Google Scholar 

  9. Finer NN, Robertson CM, Richards RT, Pinnell LE, Peters KL . Hypoxic-ischemic encephalopathy in term neonates: perinatal factors and outcome. J Pediatr 1981; 98 (1): 112–117.

    Article  CAS  Google Scholar 

  10. Ogihara T, Hirano K, Ogihara H . Non-protein-bound transition metals and hydroxyl radical generation in cerebrospinal fluid of newborn infants with hypoxic ischemic encephalopathy. Pediatr Res 2003; 53 (4): 594–599.

    Article  CAS  Google Scholar 

  11. Schmidt H, Grune T, Muller R, Siems W, Werner R . Lipoperoxidation in term new born with hypoxic isquemia encephalopathy. Pediatr Res 1996; 40: 15–20.

    Article  Google Scholar 

  12. Chumakov MV, Efremov AA, Zvereva Nlu, Dublev AV, Davydov BV, Brand laB et al. Effects of anesthetics on oxidative stress changes in patients with high anesthetic risk in the perioperative period of coronary bypass surgery. Anesteziol Reanimatol 2008; 4: 5–8.

    Google Scholar 

  13. Khinev S, Dafinova K, Tenchova V, Bakalova R . The lipid peroxidation level and antioxidant status of the plasma in patients operated on under propofol (Diprivan) anesthesia. Khirurgiia (Sofiia) 1995; 48 (5): 23–25.

    CAS  Google Scholar 

  14. Cinnella G, Vendemiale G, Dambrosio M, Serviddio G, Pugliese PL, Aspromonte G et al. Effect of propofol, sevoflurane and desflurane on systemic redox balance. Int J Immunopathol Pharmacol 2007; 20 (3): 585–593.

    Article  CAS  Google Scholar 

  15. Gathwala G, Marwah A, Gahlaut V, Marwah P . Effect of high-dose phenobarbital on oxidative stress in perinatal asphyxia: an open label randomized controlled trial. Indian Pediatr 2011; 48 (8): 613–617.

    Article  Google Scholar 

  16. Florek E, Ignatowicz E, Nowakowska A, Piekoszewski W, Kulza M, Saija A et al. Effect of combined exposure to ethanol and tobacco smoke on lipid peroxidation in rats. Przegl Lek 2009; 66 (10): 655–659.

    PubMed  Google Scholar 

  17. Dennery PA, McDonagh AF, Spitz DR, Rodgers PA, Stevenson DK . Hyperbilirubinemia results in reduced oxidative injury in neonatal Gunn rats exposed to hyperoxia. Free Radic Biol Med 1995; 19 (4): 395–404.

    Article  CAS  Google Scholar 

  18. Sarnat HB, Sarnat MS . Neonatal encephalopathy following fetal distress. A clinical and electroencephalographic study. Arch Neurol 1976; 33 (10): 696–705.

    Article  CAS  Google Scholar 

  19. Capurro HA . Simplified method for diagnosis gestational age in the newborn infant. J Pediatr 1978; 93: 120.

    Article  CAS  Google Scholar 

  20. World Medical Association. World Medical Association Declaration of Helsinki. Ethical principles for medical research involving human subjects. Nurs Ethics 2002; 9: 105–109.

    Article  Google Scholar 

  21. Jaeschke R, Guyatt GH, Sackett DL . Users’ guides to the medical literature. III. How to use an article about a diagnostic test. B. What are the results and will they help me in caring for my patients? The Evidence-Based Medicine Working Group. JAMA 1994; 271: 703–707.

    Article  CAS  Google Scholar 

  22. Jaeschke R, Guyatt G, Sackett DL . Users’ guides to the medical literature. III. How to use an article about a diagnostic test A. Are the results of the study valid? The Evidence-Based Medicine Working Group. JAMA 1994; 271: 389–391.

    Article  CAS  Google Scholar 

  23. Gunn AJ, Gunn TR, Gunning MI, Williams CE, Gluckman PD . Neuroprotection with prolonged head cooling started before post ischemic seizures in feta sheep. Pediatrics 1998; 102: 1098–1106.

    Article  CAS  Google Scholar 

  24. Gunn AJ, Gunn TR, De Haan HH, Williams CE, Gluckman PD . Dramatic neuronal rescue with prolonged selective head cooling after ischemia in fetal lambs. J Clin Invest 1997; 99: 248–256.

    Article  CAS  Google Scholar 

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Acknowledgements

We wish to thank the Centro Universitario de Investigaciones Biomédicas of the Universidad de Colima and the Departamento de Inmunología of the Centro de Investigaciones Biomédicas de Occidente in Guadalajara, Mexico, and in particular, Georgina Hernández PhD.

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Authors and Affiliations

  1. UMAE Hospital de Pediatría CMNO, Instituto Mexicano del Seguro Social, Hospital Materno Infantil Esperanza López Mateos, Secretaria de Salud Jalisco, Guadalajara, Mexico

    J C Barrera-de León

  2. Centro Universitario de los Altos, Universidad de Guadalajara, Guadalajara, Mexico

    R Cervantes-Munguía

  3. Centro Universitario de Investigaciones Biomédicas, Universidad de Colima, Colima, Mexico

    C Vásquez

  4. Hospital de Ginecología y Obstetricia, Centro Médico Nacional de Occidente, Instituto Mexicano del Seguro Social, Guadalajara, Mexico

    M A Higareda-Almaraz

  5. Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Guadalajara, Mexico

    A Bravo-Cuellar

  6. Servicio de Reumatologia, Hospital General de Zona 110, Instituto Mexicano del Seguro Social, Guadalajara, Mexico

    L González-López

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  1. J C Barrera-de León
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  2. R Cervantes-Munguía
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  4. M A Higareda-Almaraz
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Correspondence to J C Barrera-de León.

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Barrera-de León, J., Cervantes-Munguía, R., Vásquez, C. et al. Usefulness of serum lipid peroxide as a diagnostic test for hypoxic ischemic encephalopathy in the full-term neonate. J Perinatol 33, 15–20 (2013). https://doi.org/10.1038/jp.2012.38

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