Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Original Article
  • Published:

Measurement of glycated hemoglobin and glycated albumin in umbilical cord: evaluation of the glycemic control indicators in neonates

Journal of Perinatology volume 31, pages 430–433 (2011)Cite this article

Subjects

Abstract

Objective:

As neonatal blood contains a high proportion of fetal hemoglobin (HbF), it is difficult to use high-performance liquid chromatography (HPLC) method, latex-immunoturbidimetry (LA) method and enzymatic methods, which determine hemoglobin A1C (HbA1C) in order to provide the glycemic control indicators of neonates. In this study, we evaluated glycated hemoglobin (GHb) and glycated albumin (GA) as appropriate indicators of the glycemic control in the neonatal period.

Study Design:

Umbilical cord blood samples collected during delivery were subjected to measurements of GHb (HPLC methods using two different instruments, LA method, enzymatic method and affinity method) and serum GA.

Result:

HbA1C levels determined by the HPLC method, the LA method and the enzymatic method were as low as <3.0% in all the cases. Although GHb determined by the affinity method was 3.6 ± 0.2%, this method may not measure accurately the values of glycated HbF plus glycated HbA. Serum GA was 9.4 ± 1.1%.

Conclusion:

We speculate that serum GA, but not GHb, could be used as glycemic control indicators in neonates.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1

Similar content being viewed by others

References

  1. Cohen MP . Nonenzymatic glycation: a central mechanism in diabetic micro- vasculopathy? J Diabet Complications 1998; 2: 214–217.

    Article  Google Scholar 

  2. Koenig RJ, Peterson CM, Jones RL, Saudek C, Lehrman M, Cerami A . Correlation of glucose regulation and hemoglobin AIc in diabetes mellitus. N Engl J Med 1976; 295: 417–420.

    Article  CAS  Google Scholar 

  3. Bunn HF, Gabbay KH, Gallop PM . The glycosylation of hemoglobin: relevance to diabetes mellitus. Science 1978; 20: 21–27.

    Article  Google Scholar 

  4. Jeffcoate SL . Diabetes control and complications: the role of glycated haemoglobin, 25 years on. Diabet Med 2004; 21: 657–665.

    Article  CAS  Google Scholar 

  5. Bry L, Chen PC, Sacks DB . Effects of hemoglobin variants and chemically modified derivatives on assays for glycohemoglobin. Clin Chem 2001; 47: 153–163.

    CAS  PubMed  Google Scholar 

  6. Tammaro P . Neonatal diabetes. Endocr Dev 2007; 11: 70–82.

    Article  CAS  Google Scholar 

  7. Aguilar-Bryan L, Bryan J . Neonatal diabetes mellitus. Endocr Rev 2008; 29: 265–291.

    Article  CAS  Google Scholar 

  8. Sperling MA . The genetic basis of neonatal diabetes mellitus. Pediatr Endocrinol Rev 2006; 4 (Suppl 1): 71–75.

    PubMed  Google Scholar 

  9. Suzuki S, Makita Y, Mukai T, Matsuo K, Ueda O, Fujieda K . Molecular basis of neonatal diabetes in Japanese patients. J Clin Endocrinol Metab 2007; 92: 3979–3985.

    Article  CAS  Google Scholar 

  10. Yoshioka S . New metabolic parameter for diabetes-1,deoxyglucose (1,5-anhydroglucitol). Shonika 1983; 24: 405–410 (in Japanese).

    Google Scholar 

  11. Senécal PE, Douville P, Simard S, Coulombe R . The relationship between serum fructosamine and albumin. Clin Chim Acta 1988; 173: 239–241.

    Article  Google Scholar 

  12. Abe F, Yano M, Minami Y, Ueda T, Chikakiyo H, Miyamoto N et al. Alterations in fructosamine and glycated albumin levels during childhood. Ann Clin Biochem 1989; 26: 328–331.

    Article  CAS  Google Scholar 

  13. Schnedl WJ, Lahousen T, Wallner SJ, Krause R, Lipp RW . Silent hemoglobin variants and determination of HbA1c with the high-resolution program of the HPLC HA-8160 hemoglobin analyzer. Clin Biochem 2005; 38: 88–91.

    Article  CAS  Google Scholar 

  14. Chapelle JP, Teixeira J, Maisin D, Assink H, Barla G, Stroobants AK et al. Multicentre evaluation of the Tosoh HbA1c G8 analyser. Clin Chem Lab Med 2010; 48: 365–371.

    Article  CAS  Google Scholar 

  15. Guthrie R, Hellman R, Kilo C, Hiar CE, Crowley LE, Childs B et al. A multisite physician's office laboratory evaluation of an immunological method for the measurement of HbA1c . Diabetes Care 1992; 15: 1494–1498.

    Article  CAS  Google Scholar 

  16. Sakurabayashi I, Watano T, Yonehara S, Ishimaru K, Hirai K, Komori T et al. New enzymatic assay for glycohemoglobin. Clin Chem 2003; 49: 269–274.

    Article  CAS  Google Scholar 

  17. Nagisa Y, Kato K, Watanabe K, Murakoshi H, Odaka H, Yoshikawa K et al. Changes in glycated haemoglobin levels in diabetic rats measured with an automatic affinity HPLC. Clin Exp Pharmacol Physiol 2003; 30: 752–758.

    Article  CAS  Google Scholar 

  18. Moiz B, Hashmi MR, Sadaf S . Performance evaluation of ion exchange and affinity chromatography for HbA1c estimation in diabetic patients with HbD: a study of 129 samples. Clin Biochem 2008; 41: 1204–1210.

    Article  CAS  Google Scholar 

  19. Tominaga M, Makino H, Yoshino G, Kuwa K, Takei I, Aono Y et al. Japanese standard reference material for JDS Lot 2 haemoglobin A1c. I: comparison of Japan diabetes society-assigned values to those obtained by the Japanese and USA domestic standardization programmes and by the International Federation of Clinical Chemistry reference laboratories. Ann Clin Biochem 2005; 42: 41–46.

    Article  CAS  Google Scholar 

  20. Kouzuma T, Usami T, Yamakoshi M, Takahashi M, Imamura S . An enzymatic method for the measurement of glycated albumin in biological samples. Clin Chim Acta 2002; 324: 61–71.

    Article  CAS  Google Scholar 

  21. Kouzuma T, Uematsu Y, Usami T, Imamura S . Study of glycated amino acid elimination reaction for an improved enzymatic glycated albumin measurement method. Clin Chim Acta 2004; 346: 135–143.

    Article  CAS  Google Scholar 

  22. Krause JR, Stolc V, Campbell E . The effect of hemoglobin F upon glycosylated hemoglobin determinations. Am J Clin Pathol 1982; 78: 767–769.

    Article  CAS  Google Scholar 

  23. Felner EI, McGrath M . Inaccurate hemoglobin A(1C) levels in patients with type 1 diabetes and hereditary persistence of hemoglobin F. J Pediatr 2008; 153: 137–139.

    Article  CAS  Google Scholar 

  24. Weykamp CW, Penders TJ, Muskiet FA, van der Slik W . Influence of haemoglobin variants and derivatives on glycohemoglobin determinations, as investigated by 102 laboratories using 16 methods. Clin Chem 1993; 39: 1717–1723.

    CAS  PubMed  Google Scholar 

  25. Hall PM, Cawdell GM, Cook JG, Gould BJ . Measurement of glycosylated haemoglobins and glycosylated plasma proteins in maternal and cord blood using an affinity chromatography method. Diabetologia 1983; 25: 477–481.

    Article  CAS  Google Scholar 

  26. Worth R, Ashworth L, Home PD, Gerrard J, Lind T, Anderson J et al. Glycosylated haemoglobin in cord blood following normal and diabetic pregnancies. Diabetologia 1983; 25: 482–485.

    Article  CAS  Google Scholar 

  27. John WG, Webb AM, Jones AE . Glycosylated haemoglobin and glycosylated albumin in non-diabetic and diabetic mothers, and their babies. Diabet Med 1985; 2: 103–104.

    Article  CAS  Google Scholar 

  28. Abe F, Shima E, Hamai S, Nagamine Y, Hirota M, Oshima I et al. Variation in the levels of serum constituents in childhood. Tokushima J Exp Med 1987; 34: 75–81.

    CAS  PubMed  Google Scholar 

Download references

Author information

Author notes

  1. K Fujieda: Prof Kenji Fujieda deceased on 19 March 2010.

Authors and Affiliations

  1. Department of Internal Medicine, Kinki Central Hospital, Hyogo, Japan

    M Koga, J Murai & H Saito

  2. Department of Obstetrics and Gynecology, Kinki Central Hospital, Hyogo, Japan

    Y Yamada, T Mori & S Suno

  3. Department of Clinical Laboratory, Kinki Central Hospital, Hyogo, Japan

    K Takeuchi

  4. Department of Pediatrics, Asahikawa Medical College, Asahikawa, Japan

    S Suzuki & K Fujieda

  5. Department of Medicine, Nissay Hospital, Osaka, Japan

    S Kasayama

Authors
  1. M Koga
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J Murai
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. H Saito
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Y Yamada
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. T Mori
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. S Suno
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. K Takeuchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. S Suzuki
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. K Fujieda
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. S Kasayama
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to M Koga.

Ethics declarations

Competing interests

The authors declare no conflict of interest.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Koga, M., Murai, J., Saito, H. et al. Measurement of glycated hemoglobin and glycated albumin in umbilical cord: evaluation of the glycemic control indicators in neonates. J Perinatol 31, 430–433 (2011). https://doi.org/10.1038/jp.2010.144

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/jp.2010.144

Keywords

This article is cited by

Search

Advanced search

Quick links