Abstract
Using a conventional two-hybrid technique with MAWD as bait protein, a novel full-length cDNA was isolated and sequenced from a human liver cDNA library. This cDNA consists of 2575 base pairs and has a predicted open reading frame encoding 255 amino acids. Overall, it is similar to the catalytic enzyme PHZF, catalyzing the hydroxylation of phenazine-1-carboxylic acid to 2-hydroxy-phenazine-1-carboxylic acid. Polymerase chain reaction-based mapping with both a monochromosomal hybrid panel and radiation hybrid cell panels placed the gene to human chromosome 10q21.1 near the marker D10S210.
Similar content being viewed by others
Article PDF
Author information
Authors and Affiliations
Additional information
Received: December 15, 2000 / Accepted: January 22, 2001
Rights and permissions
About this article
Cite this article
Iriyama, C., Matsuda, S., Katsumata, R. et al. Cloning and sequencing of a novel human gene which encodes a putative hydroxylase. J Hum Genet 46, 289–292 (2001). https://doi.org/10.1007/s100380170081
Issue Date:
DOI: https://doi.org/10.1007/s100380170081