Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Original Article
  • Published:

A weight-of-evidence approach to identify nanomaterials in consumer products: a case study of nanoparticles in commercial sunscreens

Abstract

Nanoscale ingredients in commercial products represent a point of emerging environmental concern due to recent findings that correlate toxicity with small particle size. A weight-of-evidence (WOE) approach based upon multiple lines of evidence (LOE) is developed here to assess nanomaterials as they exist in consumer product formulations, providing a qualitative assessment regarding the presence of nanomaterials, along with a baseline estimate of nanoparticle concentration if nanomaterials do exist. Electron microscopy, analytical separations, and X-ray detection methods were used to identify and characterize nanomaterials in sunscreen formulations. The WOE/LOE approach as applied to four commercial sunscreen products indicated that all four contained at least 10% dispersed primary particles having at least one dimension <100 nm in size. Analytical analyses confirmed that these constituents were comprised of zinc oxide (ZnO) or titanium dioxide (TiO2). The screening approaches developed herein offer a streamlined, facile means to identify potentially hazardous nanomaterial constituents with minimal abrasive processing of the raw material.

This is a preview of subscription content, access via your institution

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Figure 1
Figure 2
Figure 3
Figure 4
Figure 5

Similar content being viewed by others

References

  1. U.S. EPA. Nanomaterial Case Studies: Nanoscale Titanium Dioxide in Water Treatment and in Topical Sunscreen (Final). U.S. Environmental Protection Agency, Washington, DC, EPA/600/R-09/057F, 2010. http://cfpub.epa.gov/ncea/cfm/recordisplay.cfm?deid=230972.

  2. Weir A, Westerhoff P, Fabricius L, Hristovski K, von Goetz N . Titanium dioxide nanoparticles in food and personal care products. Environ Sci Technol 2012; 46: 2242–2250.

    Article  CAS  Google Scholar 

  3. Kim T-H, Kim M, Park H-S, Shin US, Gong M-S, Kim H-W . Size-dependent cellular toxicity of silver nanoparticles. J Biomed Mater Res A 2012; 100A: 1033–1043.

    Article  CAS  Google Scholar 

  4. Johnston H, Hutchison G, Christensen F, Peters S, Hankin S, Stone V . Identification of the mechanisms that drive the toxicity of TiO2 particulates: the contribution of physicochemical characteristics. Part Fibre Toxicol 2009; 6: 33.

    Article  Google Scholar 

  5. Food and Drug Administration (FDA). Labeling and Effectiveness Testing: Sunscreen Drug Products for Over-the-Counter Human Use - Small Entity Compliance Guide. Silver Spring, MD, USA. 2012 http://www.fda.gov/Drugs/GuidanceComplianceRegulatoryInformation/Guidances/ucm330694.htm.

  6. European Commission. Commission recommendation of 18 october 2011 on the definition of nanomaterial, 2011/696/EU. Off J Eur Union 2011; L275: 38–40.

    Google Scholar 

  7. American Society for Testing and Materials (ASTM). Standard Guide for Handling Unbound Engineered Nanoscale Particles in Occupational Settings, E 2535-07. ASTM International: West Conshohocken, PA, USA. 2007.

  8. Szakal C, Roberts S, Westerhoff P, Bartholomaeus A, Buck N, Illuminato I et al. Measurement of nanomaterials in foods: integrative consideration of challenges and future prospects. ACS Nano 2014; 8: 3128–3135.

    Article  CAS  Google Scholar 

  9. Duncan TV . Applications of nanotechnology in food packaging and food safety: barrier materials, antimicrobials and sensors. J Colloid Interface Sci 2011; 363: 1–24.

    Article  CAS  Google Scholar 

  10. Schafer B, Tentschert J, Luch A . Nanosilver in consumer products and human health: more information required!. Environ Sci Technol 2011; 45: 7589–7590.

    Article  Google Scholar 

  11. Lewicka ZA, Benedetto AF, Benoit DN, Yu WW, Fortner JD, Colvin VL . The structure, composition, and dimensions of TiO2 and ZnO nanomaterials in commercial sunscreens. J Nanopart Res 2011; 13: 3607–3617.

    Article  CAS  Google Scholar 

  12. Tyner KM, Wokovich AM, Doub WH, Buhse LF, Sung L-P, Watson SS et al. Comparing methods for detecting and characterizing metal oxide nanoparticles in unmodified commercial sunscreens. Nanomedicine 2009; 4: 145–159.

    Article  CAS  Google Scholar 

  13. Noonan G, Whelton A, Carlander D, Duncan T . Measurement methods to evaluate engineered nanomaterial release from food contact materials. Compr Rev Food Sci Food Safe 2014; 13: 679–692.

    Article  CAS  Google Scholar 

  14. Guo H, Zhang Z, Xing B, Mukherjee A, Musante C, White J et al. Analysis of silver nanoparticles in antimicrobial products using surface-enhanced raman spectroscopy (SERS). Environ Sci Technol 2015; 49: 4317–4324.

    Article  CAS  Google Scholar 

  15. Hope BK, Clarkson JR . A strategy for using weight-of-evidence methods in ecological risk assessments. Hum Ecol Risk Assess 2013; 20: 290–315.

    Article  Google Scholar 

  16. Hull RN, Swanson S . Sequential analysis of lines of evidence–an advanced weight-of-evidence approach for ecological risk assessment. Integr Environ Assess Manag 2006; 2: 302–311.

    PubMed  Google Scholar 

  17. von der Kammer F, Ferguson PL, Holden PA, Masion A, Rogers KR, Klaine SJ et al. Analysis of engineered nanomaterials in complex matrices (environment and biota): general considerations and conceptual case studies. Environ Toxicol Chem 2012; 31: 32–49.

    Article  CAS  Google Scholar 

  18. Poda AR, Bednar AJ, Kennedy AJ, Harmon A, Hull M, Mitrano DM et al. Characterization of silver nanoparticles using flow-field flow fractionation interfaced to inductively coupled plasma mass spectrometry. J Chromatogr A 2011; 1218: 4219–4225.

    Article  CAS  Google Scholar 

  19. Contado C, Pagnoni A . TiO2 in commercial sunscreen lotion: flow field-flow fractionation and ICP-AES together for size analysis. Anal Chem 2008; 80: 7594–7608.

    Article  CAS  Google Scholar 

  20. Samontha A, Shiowatana J, Siripinyanond A . Particle size characterization of titanium dioxide in sunscreen products using sedimentation field-flow fractionation-inductively coupled plasma-mass spectrometry. Anal Bioanal Chem 2011; 399: 973–978.

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The use of trade, product, or firm names in this report is for descriptive purposes only and does not imply endorsement by the US Government. Permission was granted by the Chief of Engineers to publish this information. The findings of this report are not to be construed as an official Department of the Army position unless so designated by other authorized documents.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Michael F Cuddy.

Ethics declarations

Competing interests

The authors declare no conflict of interest.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Cuddy, M., Poda, A., Moser, R. et al. A weight-of-evidence approach to identify nanomaterials in consumer products: a case study of nanoparticles in commercial sunscreens. J Expo Sci Environ Epidemiol 26, 26–34 (2016). https://doi.org/10.1038/jes.2015.51

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/jes.2015.51

Keywords

This article is cited by

Search

Quick links