Abstract
Bacterial phospho-N-acetylmuramyl-pentapeptide translocase (translocase I: EC 2.7.8.13) is a key enzyme in peptidoglycan biosynthesis, and a known target of antibiotics. Here we report a novel nucleoside inhibitor against translocase I, A-94964, isolated from the culture broth of the strain Streptomyces sp. SANK 60404. A-94964 inhibited bacterial translocase I with IC50 value of 1.1 μg/ml, and showed antimicrobial activities against Staphylococcus aureus and Enterococcus faecalis with MIC of 100 and 50 μg/ml, respectively. A-94964 did not show cytotoxicity against mammalian cell lines.
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Murakami, R., Fujita, Y., Kizuka, M. et al. A-94964, a Novel Inhibitor of Bacterial Translocase I, Produced by Streptomyces sp. SANK 60404. J Antibiot 61, 537–544 (2008). https://doi.org/10.1038/ja.2008.71
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DOI: https://doi.org/10.1038/ja.2008.71
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