Glucagon-like peptide-2 (GLP-2) affects multiple facets of gastrointestinal physiology and have been used to treat patients with short bowel syndrome, but the distribution of its receptor (GLP2R) in human remains poorly understood. Gastric tissue samples of non-obese patients (NOB, n=10) and obese patients without diabetes (OB, n=31) and with diabetes (OWD, n=12) were used to evaluate GLP2R expression and distribution. Immunostaining with a validated antibody, as well as fluorescence in situ hybridization, showed that GLP2R expression was significantly increased in gastric chief cells in OB and OWD patients. PKCζ expression was also significantly increased. This is the first evidence of increased GLP2R expression in chief cells of patients with severe obesity regardless of diabetes status.
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This research is supported by grants from Shanghai Natural Science Foundation (14ZR1436600).
Feng Li conceived and designed the experiments, contributed to immunostaining and in situ hybridization experiments and wrote the manuscript; Ying Peng and Yi Zhang contributed to Western blotting experiments; Jingyang Gao contributed to acquisition of clinical data of patients; Liesheng Lu and Donglei Zhou contributed to gastric tissue samples collection; Dajin Zhou and Hui Sheng contributed to discussion; Shen Qu designed the experiments, contributed to discussion, and reviewed and edited the manuscript.
The authors declare no conflict of interest.
Supplementary Information accompanies this paper on International Journal of Obesity website