To investigate the effect of miR-145 on the phenotypic modulation in rat corpus cavernosum smooth muscle cells. Corpus cavernosum smooth muscle cells were treated with either miR-145 mimics or miR-145 negative control. Cell proliferation were analyzed by the MTS assay and colony formation assay. Wound healing assay were performed to detect the effect of miR-145 on cell migration. The mRNA and protein levels of phenotype marker proteins were assessed by quantitative real-time polymerase chain reaction and western blotting. The intracavernosal pressure and mean arterial pressure were measured to assess erectile function at one month after the injection of platelet-derived growth factor-BB and miR-145. Our results showed that miR-145 inhibited the proliferation and migration of cavernosal smooth muscle cells. Smooth muscle cell phenotypic markers were also affected by overexpression of miR-145, as indicated by the increase in α-smooth muscle actin, calponin and smooth muscle myosin heavy chain expression. Moreover, significantly attenuated erectile function was observed in the platelet-derived growth factor-BB group as compared with the platelet-derived growth factor-BB+miR-145 group. These findings indicated that miR-145 regulate phenotypic modulation of corpus cavernosum smooth muscle cells.
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This study was supported by Natural Science Foundation of Guangdong Province. (2014A030313302) and National Natural Science Foundation of China (No. 81571433).
The authors declare no conflict of interest.
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Luo, J., Liu, L., Wu, Z. et al. The effects of miRNA-145 on the phenotypic modulation of rat corpus cavernosum smooth muscle cells. Int J Impot Res 29, 229–234 (2017). https://doi.org/10.1038/ijir.2017.28