Endothelial damage is repaired by endothelial progenitor cells (EPCs), which are pivotal in preventing cardiovascular diseases and prolonging lifespan. The WHO Cardiovascular Diseases and Alimentary Comparison Study demonstrated that dietary taurine and magnesium (Mg) intake suppresses cardiovascular diseases. We herein evaluate the effects of taurine and Mg supplementation on EPC function and oxidative stress in healthy men and spontaneously hypertensive rats (SHRs). Healthy men received taurine (3 g per day) or Mg (340 mg per day) for 2 weeks. SHRs and Wistar-Kyoto (WKY) rats were housed with high-salt drinking water (1% NaCl). The SHRs received 3% taurine solution and/or a high-Mg (600 mg per 100 g) diet for 4 weeks. Their peripheral blood mononuclear cells were separated to quantify EPC colony formation. Oxidative stress markers in their peripheral blood were evaluated using a free radical analytical system and a thiobarbituric acid reactive substance (TBARS) assay. Taurine and Mg supplementation significantly increased EPC colony numbers and significantly decreased free radical levels and TBARS scores in healthy men. Taurine and Mg supplementation significantly increased EPC colony numbers and significantly decreased TBARS scores and free radical levels in SHRs. Nicotinamide adenine dinucleotide phosphate oxidase component mRNA expression was significantly higher in the renal cortex of salt-loaded SHRs than in WKY rats, in which it was suppressed by taurine and Mg supplementation. Taurine and Mg supplementation increased EPC colony formation in healthy men and improved impaired EPC function in SHRs through antioxidation, indicating that the dietary intake of taurine and Mg may prolong lifespan by preventing the progression of cardiovascular diseases.
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Shi Q, Rafii S, Wu MH, Wijelath ES, Yu C, Ishida A, Fujita Y, Kothari S, Mohle R, Sauvage LR, Moore MA, Storb RF, Hammond WP . Evidence for circulating bone marrow-derived endothelial cells. Blood 1998; 92: 362–367.
Hill JM, Zalos G, Halcox JP, Schenke WH, Waclawiw MA, Quyyumi AA, Finkel T . Circulating endothelial progenitor cells, vascular function, and cardiovascular risk. N Engl J Med 2003; 348: 593–600.
Grisar J, Aletaha D, Steiner CW, Kapral T, Steiner S, Seidinger D, Weigel G, Schwarzinger I, Wolozcszuk W, Steiner G, Smolen JS . Depletion of endothelial progenitor cells in the peripheral blood of patients with rheumatoid arthritis. Circulation 2005; 111: 204–211.
Yao EH, Yi Yu, Fukuda N . Oxidative stress on progenitor and stem cells in cardiovascular diseases. Curr Pharm Biotechnol 2006; 7: 101–108.
Yao EH, Fukuda N, Matsumoto T, Katakawa M, Yamamoto C, Tsunemi A, Suzuki R, Ueno T, Matsumoto K . Losartan improves the impaired function of endothelial progenitor cells in hypertension via an antioxidant effect. Hypertens Res 2007; 30: 1119–1128.
Yu Y, Fukuda N, Yao EH, Matsumoto T, Kobayashi N, Suzuki R, Tahira Y, Ueno T, Matsumoto K . Effects of an ARB on endothelial progenitor cell function and cardiovascular oxidation in hypertension. Am J Hypertens 2008; 21: 72–77.
Yoshida Y, Fukuda N, Maeshima A, Yamamoto C, Matsumoto T, Ueno T, Nojima Y, Matsumoto K, Soma M . Treatment with valsartan stimulates endothelial progenitor cells and renal label-retaining cells in hypertensive rats. J Hypertens 2011; 29: 91–101.
Matsumura M, Fukuda N, Kobayashi N, Umezawa H, Takasaka A, Matsumoto T, Yao E-H, Ueno U, Negishi N . Effects of atorvastatin on angiogenesis in hindlimb ischemia and endothelial progenitor cell formation in rats. J Atheroscler Thromb 2009; 16: 319–326.
Suzuki R, Fukuda N, Katakawa M, Tsunemi A, Tahira Y, Matsumoto T, Ueno T, Soma M . Effects of an angiotensin II receptor blocker on the impaired function of endothelial progenitor cells in patients with essential hypertension. Am J Hypertens 2014; 27: 695–701.
Hennig B, Alvarado A . Nutrition and endothelial cell integrity: implications in atherosclerosis. Prog Food Nutr Sci 1993; 17: 119–157.
Jialal I, Norkus EP, Cristol L, Grundy SM . β-Carotene inhibits the oxidative modification of low-density lipoprotein. Biochim Biophys Acta 1991; 1086: 134–138.
Ulrich-Merzenich G, Zeitler H, Vetter H, Bhonde RR . Protective effects of taurine on endothelial cells impaired by high glucose and oxidized low density lipoproteins. Eur J Nutr 2007; 46: 431–438.
Yu X, Chen K, Wei N, Zhang Q, Liu J, Mi M . Dietary taurine reduces retinal damage produced by photochemical stress via antioxidant and anti-apoptotic mechanisms in Sprague-Dawley rats. Br J Nutr 2007; 98: 711–719.
Yamori Y, Liu L, Mizushima S, Ikeda K, Nara Y, Simpson FO . Male cardiovascular mortality and dietary markers in 25 population samples of 16 countries. J Hypertens 2006; 24: 1499–1505.
Hu TH, Lin CL, Huang YW, Liu PE, Hwang DF . Dietary amino acid taurine ameliorates liver injury in chronic hepatitis patients. Amino Acids 2008; 35: 469–473.
Yamori Y, Nara Y, Kihara M, Mano M, Horie R . Simple method for sampling consecutive 24-hour urine for epidemiological and clinical studies. Clin Exp Hypertens A 1984; 6: 1161–1167.
Yamori Y, Liu L, Mori M, Sagara M, Murakami S, Nara Y, Mizushima S . Taurine as the nutritional factor for the longevity of the Japanese revealed by a world-wide epidemiological survey. Adv Exp Med Biol 2009; 643: 13–25.
Kitiyakara C, Chabrashvili T, Chen Y, Blau J, Karber A, Aslam S, Welch WJ, Wilcox CS . Salt intake, oxidative stress, and renal expression of NADPH oxidase and superoxide dismutase. J Am Soc Nephrol 2003; 14: 2775–2782.
Kanaokaa Y, Inagaki E, Hamanaka S, Masaki H, Tanemotob K . Analysis of reactive oxygen metabolites (ROMs) after cardiovascular surgery as a marker of oxidative stress. Acta Med Okayama 2010; 64: 323–330.
Xu YJ, Arneja AS, Tappia PS, Dhalla NS . The potential health benefits of taurine in cardiovascular disease. Exp Clin Cardiol 2008; 13: 57–65.
Yao EH, Fukuda N, Matsumoto T, Yamamoto C, Suzuki R, Ueno T, Kobayashi N, Matsumoto K . Effects of the antioxidative β-blocker celiprolol on endothelial progenitor cells and oxidation in spontaneously hypertensive rats. Am J Hypertens 2008; 21: 1062–1068.
Fukuda N, Satoh C, Hu WY, Soma M, Kubo A, Kishioka H, Watanabe Y, Izumi Y, Kanmatsuse K . Production of angiotensin II by homogeneous cultures of vascular smooth muscle cells from spontaneously hypertensive rats. Arterioscler Thromb Vasc Biol 1999; 19: 1210–1217.
Kawada N, Imai E, Karber A, Welch WJ, Wilcox CS . A mouse model of angiotensin II slow pressor response: role of oxidative stress. J Am Soc Nephrol 2002; 13: 2860–2868.
Hu WY, Fukuda N, Kanmatsuse K . Growth characteristics, angiotensin II-generation, and microarray-determined gene expression in vascular smooth muscle cells from young spontaneously hypertensive rats. J Hypertens 2002; 20: 1323–1333.
Nyby MD, Abedi K, Smutko V, Eslami P, Tuck ML . Vascular angiotensin type 1 receptor expression is associated with vascular dysfunction, oxidative stress and inflammation in fructose-fed rats. Hypertens Res 2007; 30: 451–457.
Taniyama Y, Griendling KK . Reactive oxygen species in the vasculature: molecular and cellular mechanisms. Hypertension 2003; 42: 1075–1081.
Balin AK, Fisher AJ, Anzelone M, Leong I, Allen RG . Effects of establishing cell cultures and cell culture conditions on the proliferative life span of human fibroblasts isolated from different tissues and donors of different ages. Exp Cell Res 2002; 274: 275–287.
The present study was supported by financial grants from the ‘Strategic Research Base Development’ Program for Private Universities for 2008–2012 (S0801033), Nihon University Multidisciplinary Research Grant for 2010–2011 (sou10-045). Program for Private Universities for 2014–2018 (S1411018). The grants were subsidized by MEXT.
The authors declare no conflict of interest.
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Katakawa, M., Fukuda, N., Tsunemi, A. et al. Taurine and magnesium supplementation enhances the function of endothelial progenitor cells through antioxidation in healthy men and spontaneously hypertensive rats. Hypertens Res 39, 848–856 (2016). https://doi.org/10.1038/hr.2016.86
- endothelial progenitor cell
- oxidative stress
- spontaneously hypertensive rat
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