Abstract
A genetic marker map of Atlantic salmon would facilitate the identification of loci influencing economically important traits. In the present paper we describe five new Atlantic salmon microsatellites. Segregation studies and linkage analysis of these and previously published microsatellites were carried out in pedigrees consisting of diploid dams and haploid gynogenetic offspring. We confirm earlier reports that salmon microsatellites tend to have a higher number of repeat units than those of mammals. Linkage analysis revealed that three microsatellites belong to a linkage group spanning ≍ 50 cM of the genome, whereas the remaining 10 markers seem to be unlinked.
Similar content being viewed by others
Article PDF
References
Altman, D G. 1991. Practical Statistics for Medical Research, Chapman and Hall, London.
Botstein, D, White, R L, Skolnick, M, and Davis, R W. 1980. Construction of a genetic linkage map in man using restriction fragment length polymorphism. Am J Hum Genet, 32, 314–331.
Brooker, A L, Cook, D, Bentzen, P, Wright, J M, and Doyle, R W. 1994. Organization of microsatellites differs between mammals and coldwater teleost fishes. Can J Fish Aquat Sci, 51, 1959–1966.
Davisson, M T, Wright, J E, and Atherton, L M. 1973. Cytogenetic analysis of pseudolinkage of LDH loci in the teleost genus Salvelinus. Genetics, 73, 645–658.
Estoup, A, Presa, P, Krieg, F, Vaiman, D, and Guyomard, R. 1993. (CT)n and (GT)n microsatellites: a new class of genetic markers for Salmo trutta L. (brown trout). Heredity, 71, 488–496.
Franck, J P, Harris, A, Bentzen, P, Denovan-Wright, E M, and Wright, J. 1991. Organization and evolution of satellite, minisatellite and microsatellite DNAs in teleost fishes. Oxford Surv Eukaryotic Genes, 7, 51–82.
Johnson, K R, Wright, J E, and May, A. 1987. Linkage relationships reflecting ancestral tetraploidy in salmonid fish. Genetics, 116, 579–591.
Lander, E, Green, P, Abrahamson, J, Barlow, A, Daley, M, Lincoln, S, and Newburg, L. 1987. MAPMAKER: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics, 1, 174–181.
Lie, Ø, Slettan, A, Lingaas, F, Olsaker, I, Hordvik, I, and Refstie, T. 1994. Haploid gynogenesis: A powerful strategy for linkage analysis in fish. Anim Biotech, 5, 33–45.
May, B, and Johnson, K R. 1990. Composite linkage map of salmonid fishes (Salvelinus, Salmo, Onchorynchus). In: O'Brien, S. J. (ed.) Genetic Maps: Locus Maps of Complex Genomes. Book 4, Nonhuman Vertebrates, pp. 151–159. Cold Spring Harbor Laboratories, Cold Spring Harbor, NY.
McConell, S, O'Reilly, P, Hamilton, L, Wright, J, and Bentzen, P. 1995. Polymorphic microsatellite loci from Atlantic salmon (Salmo salar): genetic differentiation of North American and European populations. Can J Fish Aqual Sci, 52, 1863–1872.
Ohno, S, Muramoto, J, Klein, J, and Atkin, N R. 1969. Diploid-tetraploid relationship in clupleoid and salmonid fish. In: Darlington, C. D. and Lewis, K. R. (eds) Chromosomes Today, vol. II, pp. 139–147. Oliver and Boyd, Edinburgh.
Ostrander, E A, Jong, P M, Rine, J, and Duyk, G. 1992. Construction of small-insert genomic DNA libraries highly enriched for microsatellite repeat sequences. Proc Natl Acad Sci USA, 89, 3419–3423.
Prodöhl, P A, Taggart, J B, and Ferguson, A. 1994. Single locus inheritance and joint segregation analysis of minisatellite (VNTR) DNA loci in brown trout (Salmo trutta L.). Heredity, 73, 556–566.
Refstie, T, Stoss, J, and Donaldson, E M. 1982. Production of all-female coho salmon (Oncotynchus kisutch) by diploid gynogenesis using irradiated sperm and cold shocks. Aquaculture, 29, 67–82.
Rubinsztein, D C, Amos, W, Leggo, J, Goodburn, S, Jain, S, Li, S H. et al. 1995. Microsatellite evolution — evidence for directionality and variation in rate between species. Nature Genetics, 10, 337–343.
Slettan, A, Olsaker, I, and Lie, Ø. 1993. Isolation and characterization of variable (GT)n repetitive sequences from Atlantic salmon, Salmo salar L. Anim Genet, 24, 195–197.
Slettan, A, Olsaker, I, and Lie, Ø. 1995a. Atlantic salmon, Salmo salar, microsatellites at the SSOSL25, SSOSL85, SSOSL311, SSOSL417 loci. Anim Genet, 26, 281–282.
Slettan, A, Olsaker, I, and Lie, Ø. 1995b. A polymorphic dinucleotide repeat microsatellite in Atlantic salmon, Salmo salar (SSOSL436). Genet, 26, 368.
Slettan, A, Olsaker, I, and Lie, Ø. 1996. Polymorphic Atlantic salmon (Salmo salar L.) microsatellites at the SSOSL438, SSOSL439 and SSOSL444 loci. Anim Genet, 27, 57–58.
Taggart, J B, and Ferguson, A. 1990a. Minisatellite DNA fingerprints of salmonid fishes. Anim Genet, 21, 377–389.
Taggart, J B, and Ferguson, A. 1990b. Hypervariable minisatellite DNA single locus probes for the Atlantic salmon, Salmo salar L. J Fish Biol, 37, 991–993.
Taggart, J B, Prodöhl, P A, and Ferguson, A. 1995. Genetic markers for Atlantic salmon (Salmo salar L.): single locus inheritance and joint segregation analyses of minisatellitee (VNTR) DNA loci. Anim Genet, 26, 13–20.
Thorgaard, G H, Allendorf, F W, and Knudsen, K L. 1983. Gene-centromere mapping in rainbow trout: high interference over long map distances. Genetics, 103, 771–783.
Troyer, D L, Goad, D W, Xie, H, Rohrer, G A, Alexander, L J, and Beattie, C W. 1994. Use of direct in situ single-copy (DISC) PCR to physically map five porcine microsatellites. Cytogenet Cell Genet, 67, 199–204.
Weber, J L. 1990. Informativeness of human (dC-dA)n-(dG-dT)n polymorphisms. Genomics, 7, 524–530.
Wright, J E, Johnson, K, Hollister, A, and May, B. 1983. Meiotic models to explain classical linkage, pseudolinkage and chromosome pairing in tetraploid derivative salmonid genomes. In: Rattazzi, M. C., Scandalios, J. G. and Whitt, G. S. (eds) Isozymes: Current Topics in Biological and Medical Research, vol. 10, pp. 239–260. Alan R. Liss, New York.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Slettan, A., Olsaker, I. & Lie, Ø. Segregation studies and linkage analysis of Atlantic salmon microsatellites using haploid genetics. Heredity 78, 620–627 (1997). https://doi.org/10.1038/hdy.1997.101
Received:
Issue Date:
DOI: https://doi.org/10.1038/hdy.1997.101
Keywords
This article is cited by
-
The role of temporal reproductive isolation, trophic polymorphism and growth rate fluctuations in the diversification of Arctic charr Salvelinus alpinus (L.) in Lake Kalarskii Davatchan, Transbaikalia, Russia
Hydrobiologia (2024)
-
Trophic-based diversification in benthivorous charrs (Salvelinus) dwelling littoral zones of Northern lakes
Hydrobiologia (2021)
-
Interaction among morphological, trophic and genetic groups in the rapidly radiating Salvelinus fishes from Lake Kronotskoe
Evolutionary Ecology (2020)
-
First Haploid Genetic Map Based on Microsatellite Markers in Senegalese Sole (Solea senegalensis, Kaup 1858)
Marine Biotechnology (2015)
-
The Swedish Arctic charr breeding programme
Hydrobiologia (2010)