Abstract
DNA isolated from Black Mexican sweet maize populations segregating for the presence of B chromosomes was used for PCR amplifications of 320 RAPD primers. Seventeen primers produced detectable polymorphisms between populations known to carry B chromosomes and populations devoid of B chromosomes. To ensure that the primers which generated a polymorphic band were indeed identifying B chromosomes, five Arizona Indian corn populations, three tetraploid corn populations and two High Loss corn lines differing in their B chromosome composition were analysed. Four primers were selected to screen these maize populations. Root tip counts were performed on the lines to confirm the association of the RAPD markers to the presence of B chromosomes.
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Alfenito, M R, and Birchler, J A. 1993. Molecular characterization of a maize B chromosome centric sequence. Genetics, 135, 589–597.
Beckett, J B. 1978. B-A translocations in maize. Crit Rev Genet, 12, 5.
Birchler, J A, Chalfoun, D J, and Levin, D M. 1990. Recombination in the B chromosome of maize to produce A-B-A chromosomes. Genetics, 126, 723–733.
Carlson, W R. 1988. B chromosomes as a model system for nondisjunction. In: Vig, B. and Sanberg, A. A. (eds) Aneuploidy, Part B: Induction and Test Systems, pp. 199–207. Alan R. Liss, New York.
Carlson, W R, and Roseman, R R. 1992. A new property of the maize B chromosome. Genetics, 131, 211–223.
Devos, K M, and Gale, M D. 1992. The use of random amplified polymorphic DNA markers in wheat. Theor Appl Genet, 84, 567–572.
Iqbal, M J, and Rayburn, A L. 1995. Identification of the IRS rye chromosomal segment in wheat by RAPD analysis. Theor Appl Genet, 91, 1048–1053.
King, I P, Purdue, K A, Rezanoor, H N, Koebner, R M D, Miller, T E, Reader, S M, and Nicholson, P. 1993. Characterization of Thinopyrum bessarabicum chromosome segments in wheat using random amplified polymorphic DNAs (RAPDs) and genomic in situ hybridization. Theor Appl Genet, 86, 895–900.
Porter, H L, and Rayburn, A L. 1990. B-chromosome and C-band heterochromatin variation in Arizona maize populations adapted to different altitudes. Genome, 33, 659–662.
Randolph, L F. 1941. Genetic characteristics of the B chromosomes in maize. Genetics, 26, 608–631.
Rieger, R, Michaelis, A, and Green, M M. 1976. In:Glossary of Genetics and Cytogenetics Classical and Molecular, fourth completely revised edition, pp. 49–50. Springer, Berlin.
Rogers, O S, and Bendich, A J. 1988. Extraction of DNA from plant tissue. In: Glevin, S. B. and Schilperoort, R. A. (eds) Plant Molecular Biology Manual. Kluwer Academic Publishers, Dordrecht.
Roman, H. 1947. Mitotic nondisjunction in the case of interchanges involving the B-type chromosome in maize. Genetics, 32, 391.
Roman, H. 1948. Directed fertilization in maize. Proc Natl Acad Sci USA, 34, 36–42.
Welsh, J, and McClelland, M. 1990. Fingerprinting genomes using PCR with arbitrary primers. Nucl Acids Res, 18, 7213–7218.
Williams, J G K, Kubelik, A R, Levak, K J, Rafalski, J A, and Tingey, S V. 1990. DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucl Acids Res, 18, 6531–6535.
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Gourmet, C., Lane Rayburn, A. Identification of RAPD markers associated with the presence of B chromosomes in maize. Heredity 77, 240–244 (1996). https://doi.org/10.1038/hdy.1996.137
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DOI: https://doi.org/10.1038/hdy.1996.137