Abstract
Chromosome instability was monitored through time in cell suspensions of the diploids Triticum tauschii (the D genome donor) and T. monococcum (the A genome donor), the tetraploids, T. durum and T. dicoccum (both AABB) and in the cultivars Copain and Sicco of hexaploid (AABBDD) breadwheat (T. aestivum). Differences in stability were observed between the different ploidy levels and between species within the ploidy levels. The diploids were most stable, particularly T. tauschii, and the hexaploids were least stable. Instability was greatest in all the lines during the first months of culture. In all but one line, very few structural aberrations were observed, even after many months in culture. Morphogenetic capacity was lost in all lines, even though some retained high proportions of euploid cells. The relevance of these results is discussed in relation to the regeneration of breadwheat from protoplasts.
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Abdullah, R, Thompson, J A, Rush, G S, Kaushik, R P, and Cocking, E. 1989. Protoclonal variation in the seed progeny of plants regenerated from rice protoplasts. Plant Sei, 65, 97–102.
Bayliss, M W. 1980. Chromosomal variation in plant tissues in culture. Int Rev Cytol, 11A, 113–144.
Chang, Y F, Wang, W C, Warfield, C Y, Nguyen, H T, and Wong, J R. 1991. Plant regeneration from protoplasts isolated from long-term cell cultures of wheat (Triticum aestivum L.). Plant Cell Rep, 9, 611–614.
Constantin, M J. 1981. Chromosome instability in cell and tissue cultures and regenerated plants. Env Exp Bot, 21, 359–368.
Galiba, G, Kovács, G, and Sutka, J. 1986. Substitution analysis of plant regeneration from callus culture in wheat. Plant Breed, 97, 261–263.
Harris, R, Wright, M, Byrne, M, Varnum, J, Brightwell, B, and Schubert, K. 1988. Callus formation and plantlet regeneration from protoplasts derived from suspension cultures of wheat (Triticum aestivum L.). Plant Cell Rep, 7, 337–340.
Hashim, Z N, Cambbell, W F, and Carman, J G. 1991. Normalization of the DNA content of telophase cells from wheat calli by nutrient modifications. Theor Appl Genet, 82, 413–416.
He, D G, Yang, Y M, and Scott, K J. 1992. Plant regeneration from protoplasts of wheat (Triticum aestivum cv. Hartog). Plant Cell Rep, 11, 16–19.
Higgins, P, and Mathias, R J. 1987. The effect of the 4B chromosome of hexaploid wheat on the growth and regeneration of callus cultures. Theor Appl Genet, 72, 439–444.
Kao, K N, Miller, R A, Gamborg, O L, and Harvey, B L. 1970. Variation in chromosome number and structure in plant cells grown in suspension cultures. Can J Genet Cytol, 12, 279–301.
Karp, A. 1991. On the current understanding of somaclonal variation. Oxford Surv Plant Mol Cell Biol, 7, 1–58.
Karp, A, and Maddock, S E. 1984. Chromosome variation in wheat plants regenerated from cultured immature embryos. Theor Appl Genet, 67, 249–255.
Karp, A, Nelson, R S, Thomas, E, and Bright, S W J. 1982. Chromosome variation in protoplast-derived potato plants. Theor Appl Genet, 63, 265–272.
Karp, A, Wu, Q S, Steele, S H, and Jones, M G K. 1987. Chromosome variation in dividing protoplasts and cell suspensions of wheat. Theor Appl Genet, 74, 140–146.
Larkin, P J, and Scowcroft, W R. 1981. Somaclonal variation —a novel source of variability from cell cultures for plant improvement. Theor Appl Genet, 60, 197–214.
Lazar, M D, Collins, G B, and Vian, W E. 1983. Genetic and environmental effects on the growth and differentiation of wheat somatic cell cultures. J Hered, 74, 353–357.
Li, Z Y, Guang-Min, X, Hui-Min, C, and Guang-Qin, G. 1992. Plant regeneration from protoplasts derived from embryo-genesis suspension cultures of wheat (Triticum aestivum L.). J Plant Physiol 139, 714–718.
Maddock, S E, Lancaster, R, Risiott, R, and Franklin, J. 1983. Plant regeneration from cultured immature embryos and inflorescences of 25 cultivars of wheat (Triticum aestivum). J Expt Bot, 34, 915–926.
Murashige, T, and Nakano, R. 1967. Chromosome complement as a determinant of the morphological potential of tobacco cells. Am J Bot, 54, 963–970.
Murashige, T, and Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Planta, 15, 473–497.
Norstog, K, Wendell, E, Wall, E, and Howard, G P. 1969. Cytological characteristics of ten-year-old rye-grass endosperm tissue cultures. Bot Gaz, 130, 83–86.
Pijnacker, L P, Hermelink, J H M, and Ferwerda, M A. 1986. Variability of DNA content and karyotype in cell cultures of an interdihaploid Solanum tuberosum. Plant Cell Rep, 5, 43–46.
Singh, B D, and Harvey, B L. 1975. Selection for diploid cells in suspension cultures of Happlopappus gracilis. Nature, 253, 453.
Singh, R J. 1986. Chromosomal variation in immature embryo derived calluses of barley (Hordeum vulgare L.). Theor Appl Genet, 72, 710–716.
Vasil, V, Redway, F, and Vasil, I K. 1990. Regeneration of plants from embryogenic suspension culture protoplasts of wheat (Triticum aestivum L.). Bioj Technol, 8, 429–434.
Ziauddin, A, and Kasha, K J. 1990. Long-term callus cultures of diploid barley (Hordeum vulgare).II. Effects of auxins on chromosomal status of cultures and regeneration of plants. Euphylica, 48, 279–286.
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Winfield, M., Davey, M. & Karp, A. A comparison of chromosome instability in cell suspensions of diploid, tetraploid and hexaploid wheats. Heredity 70, 187–194 (1993). https://doi.org/10.1038/hdy.1993.28
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DOI: https://doi.org/10.1038/hdy.1993.28
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