Abstract
Multilocus protein electrophoresis was used to estimate genetic variability in a sample of 38 Italian wolves (Canis lupus). Percentage of polymorphic loci was p = 10.0 per cent (four polymorphic loci out of 40 examined), and average observed heterozygosity was \(\overline{Ho}\) = 0.028. Genotypes were in Hardy-Weinberg equilibrium. Electrophoretic analysis does not indicate a significant reduction of genetic variability at nuclear gene loci following at least one century of isolation from other European populations and demographic fluctuations suggested by recent range contraction and expansion. These findings are compared with published allozyme and mitochondrial DNA data for dogs, Canadian wolves, and introgressed wolf × coyote populations from Minnesota and Isle Royale (U.S.A.).
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Arnold, I C J, and Bouw, J. 1985. Biochemical variants in dogs. Anim Blood Grps Biochem Genet, 16, Suppl. 1 54–55 (abst).
Arnold, I C J, and Bouw, J. 1989. A new allele of glucose phosphate isomerase in dogs. Anim Genet, 20, 217–220.
Boitani, L. 1984. Genetic considerations on wolf conservation in Italy. Boll Zool, 51, 367–373.
Boitani, L. 1992. Wolf research and conservation in Italy. Biol Cons, 61, 125–132.
Boitani, L, Ciucci, P, Corsi, F, and Fabbri, M L. 1989. Assessing actual and potential wolf range in Italy with the aid of a GIS. In: 5th Intl Theriol Conf Abstracts, Rome.
Boscagli, G. 1991. Evoluzione del nucleo di lupi appenninici (Canis lupus italicus) in cattività nel Parco Nazionale d'Abruzzo e situazione della popolazione italiana di lupo. Situazione della popolazione di orso (Ursus arctos marsicanus) in Appennino centrale. In: Randi, E. and Spagnesi, M. (eds.) Genetics and Wildlife Conservation. Suppl. Ric. Biol. Selvaggina. XVIII, pp. 219–225.
Braend, M, and Roed, K H. 1987. Polymorphism of transferrin and esterase in Alaskan wolves: evidence of close molecular homology with the dog. Anim Genet, 18, 143–148.
Cagnolaro, L, Rosso, D, Spagnesi, M, and Venturi, B. 1974. Inchiesta sulla distribuzione del lupo (Canis lupus) in Italia e nei Cantoni Ticino e Grigioni (Svizzera). Ric Biol Selvaggina, 59, 1–75.
Ciucci, P, and Boitani, L. 1991. Viability assessment of the Italian wolf and guidelines for the management of the wild and a captive population. Ric Biol Selvaggina, 89, 1–58.
Council of Europe. 1990. Status and conservation needs of the wolf in the Council of Europe member states (M. Delibes, ed.). Nature and Environment Series, Strasbourg, 47, 1–46.
Davis, B J. 1964. Disc electrophoresis. II. Method and application to human serum proteins. Ann N Y Acad Sci, 121, 404–427.
Ferrel, R A, Morizot, D C, Horn, J, and Carley, C J. 1980. Biochemical markers in a species endangered by introgression: the red wolf. Biochem Genet, 18, 39–49.
Fisher, R A, Putt, W, and Hackel, E. 1976. An investigation of the products of 53 gene loci in three species of wild Canidae: Canis lupus, Canis latrans and Canis familiaris. Biochem Genet, 14, 963–974.
Francisci, R, Ciucci, P, Andreoli, G, and Boitani, L. 1992. Storfa naturale di un gruppo di card inselvatichiti nell'areale di presenza del lupo in Marsica. Unpublished final report to the Italian Institute for Wildlife Biology (in Italian).
Francisci, F, and Guberti, V. 1993. Recent trends of wolves in Italy as apparent from kill figures and specimens. Paper accepted for proceedings of symposium Wolves in Europe: current status and prospects Oberammergau (FRG), 2–5 April 1992, Wildbiologische Gesellschaft Muenchen.
Gilpin, M. 1991. Intellectual challenges of biodiversity conservation. In: Randi, E. and Spagnesi, M. (eds.) Genetics and Wildlife Conservation. Suppl. Ric. Biol. Selvaggina. XVIII, pp. 19–27.
Harris, H, and Hopkinson, D A. 1976. Handbook of Enzyme Electrophoresis in Human Genetics. North Holland Publ. Co., Amsterdam.
Hartl, D L, and Clark, A G. 1989. Principles of Population Genetics. Sinauer, Sunderland.
Jolley, W B, and Allen, H W. 1965. Formation of complexes between basic proteins of leucocytes and plasma globulins. Nature, 208, 390–391.
Kennedy, P K, Kennedy, M L, Clarkson, P L, and Leipins, I S. 1991. Genetic variability in natural populations of the gray wolf, Canis lupus. Can J Zool, 69, 1183–1188.
Lehman, N, Eisenhawer, A, Hansen, K, Mech, D L, Peterson, R O, Gogan, P J P, and Wayne, R K. 1991. Introgression of coyote mitochondrial DNA into sympatric North American gray wolf populations. Evolution, 45, 104–119.
Meera, Khan, Los, W R T, Does, J A, and Epstein, R B. 1973. Isoenzyme markers in dog blood cells. Transplantation, 15, 624–628.
Mendelssohn, M. 1982. Wolves in Israel. In: Harrington & Paquet (eds) Wolves of the World. Noyes Publications, Park Ridge, NY, pp. 173–195.
Nei, M, Maruyama, T, and Chakraborty, R. 1975. The bottleneck effect and genetic variability in populations. Evolution, 29, 1–10.
Seal, U S. 1975. Molecular approaches to taxonomic problems in the Canidae. In: Fox, M. W. (ed.) The Wild Canids: their systematics, behavioral ecology and evolution. Van Nostrand Reinhold Co., New York, pp. 27–39.
Simonsen, V. 1976. Electrophoretic studies on the blood proteins of domestic dogs and other Canidae. Hereditas, 82, 7–18.
Sokal, R R, and Rohlf, R J. 1981. Biometry, W. H. Freeman, San Francisco.
Swofford, D L, and Selander, R K. 1989. BIOSYS-1. A computer program for the analysis of allelic variation in population genetics and biochemical systematics. Release. 1.7. Illinois.
Wayne, R K, and O'Brien, S J. 1987. Allozyme divergence within the Canidae. Syst Zool, 36, 339–355.
Wayne, R K, Gilbert, D A, Eisenhawer, A, Lehman, N, Hansen, K, Girman, D, Peterson, R O, Mech, L D, Gogan, P J P, Seal, U S, and Krumenacker, R J. 1991. Conservation genetics of the endangered Isle Roy ale gray wolf. Cons Biol, 5, 41–51.
Wayne, R K, Lehman, N, Allard, M W, and Honeycutt, R L. 1992. Mitochondrial DNA variability of the gray wolf: genetic consequences of population decline and habitat fragmentation on genetic variability. Cons Biol, in press.
Weiden, P, Storb, R, Kolb, H J, Graham, T, Anderson, J, and Giblett, E. 1974. Genetic variation of red blood cell enzymes in the dog. Transplantation, 17, 115–120.
Weir, B S. 1990. Genetic Data Analysis, Sinauer Associates, Sunderland, Massachusetts.
Williams, D E, and Reisfeld, R A. 1964. Disc electrophoresis in Polyacrylamide gels: extension to new conditions of pH and buffers. Ann N Y Acad Sci, 121, 373–381.
Zimen, E. 1978. Der Wolf, Mythos und Verhalten, Meyster Verlag, Wien.
Zimen, E, and Boitani, L. 1975. Number and distribution of wolves in Italy. Z Saugetierk, 40, 102–112.
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Randi, E., Lucchini, V. & Francisci, F. Allozyme variability in the Italian wolf (Canis lupus) population. Heredity 71, 516–522 (1993). https://doi.org/10.1038/hdy.1993.170
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DOI: https://doi.org/10.1038/hdy.1993.170