Abstract
The genetics of arsenate tolerance in Holcus lanatus is investigated. Three tolerant plants from an abandoned arsenic mine, and three non-tolerant plants and one less tolerant plant (C9) from an uncontaminated site were crossed. Five polycrosses between plants from F1 crosses between mine and non-tolerants were set up. Four polycrosses between tolerant F2 progeny, and four polycrosses between non-tolerant F2 progeny, were established. A polycross involving the progeny of a single tolerant plant allowed to cross at random with a normal population was also established. The results are broadly compatible with a single-gene model for tolerance, with tolerance being dominant. The majority of F2 crosses segregated in to 3:1 ratios, and backcrosses gave 1:1 ratios. The crosses between C9 and non-tolerants gave 1:1 ratios, which suggests that the less tolerant C9 was heterozygous for tolerance. All crosses between non-tolerants gave all non-tolerant offspring. In one cross a major gene for albinism also segregated, and linkage of the tolerance gene to this gene (r.f. = 35%) was demonstrated. A number of families produced progeny ratios incompatible with the simple major gene model. Possible causes of these anomalous crosses are discussed and it is suggested that the tolerance gene may show variable penetrance depending on the genetic background.
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Antonovics, J, Bradshaw, A D, and Turner, R J. 1971. Heavy metal tolerance in plants. Adv Ecol Res, 7, 1–85.
Beddows, A R. 1961. Biological Flora of the British Isles: Holcus lanatus L. J Ecol, 49, 421–430.
Bradshaw, A D, and McNeilly, T. 1981. Evolution and Pollution. Edward Arnold, London.
Cogliatti, D H, and Santa Maria, G E. 1990. Influx and efflux of phosphate in roots of wheat plants in non-growth limiting conditions. J Exp Bot, 41, 601–607.
Everitt, B S. 1977. The Analysis of Contingency Tables. Chapman and Hall, London.
Hewitt, E J. 1966. Sand and Water Culture Methods Used in the Study of Plant Nutrition. 2nd edn, Technical communication no 22, Commonwealth Agricultural Bureaux, Farnham Royal.
Ingram, C. 1987. The Evolutionary Basis of the Ecological Amplitude of Plant Species. PhD Thesis, University of Liverpool.
MacNair, M R. 1983. The genetic control of copper tolerance in the yellow monkey flower, Mimulus guttatus. Heredity, 50, 283–293.
MacNair, M R. 1990. The genetics of metal tolerance in natural populations. In: Shaw, J. (ed.) Heavy Metal Tolerance in Plants: Evolutionary Aspects. CRC Press, Boca Raton, pp. 235–254.
MacNair, M R. 1991. Why the evolution of resistance to anthropogenic toxins normally involves major gene changes: the limits to natural selection. Genetic, 84, 213–219.
MacNair, M R, and Cumbes, Q J. 1987. Evidence that arsenic tolerance in Holcus lanatus L. is caused by an altered phosphate uptake system. New Phytol, 107, 387–394.
McPharlin, I R, and Bieleski, R C. 1987. Phosphate uptake by Spirodela and Lemna during early stages of phosphate deficiency. Aust J Plant Physiol, 14, 561–572.
Meharg, A A, and MacNair, M R. 1990. An altered phosphate uptake system in arsenate tolerant Holcus lanatus L. New Phytol, 116, 29–35.
Meharg, A A, and MacNair, M R. 1992a. Genetic isolation between arsenate tolerance and the rate of influx of arsenate and phosphate in Holcus lanatus L. Heredity, 69, 336–341.
Meharg, A A, and MacNair, M R. 1992b. Suppression of the high affinity phosphate uptake system: a mechanism of arsenate tolerance in Holcus lanatus L. J Exp Bot 43, 519–524.
Porter, E K, and Peterson, P J. 1977. Arsenic tolerance in grasses growing on mine waste. Environ Poll, 14, 255–265.
Schat, H, and Ten Bookum, W M. 1992. Genetic control of copper tolerance in Silene vulgaris. Heredity, 68, 219–229.
Shaw, J. 1990. Heavy Metal Tolerance in Plants: Evolutionary Aspects. CRC Press, Boca Raton.
Symeonidis, L, McNeilly, T, and Bradshaw, A D. 1985. Inter-population variation in tolerance to cadmium, copper, lead, nickel and zinc in nine populations of Agrostis capillaris L. New Phytol, 101, 317–324.
Watkins, A J, and MacNair, M R. 1991. Genetic of arsenic tolerance in Argrostis capillaris L. Heredity, 66, 47–54.
Wilkins, D A. 1978. The measurement of tolerance to edaphic factors by means of root growth. New Phytol, 80, 623–633.
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MacNair, M., Cumbes, Q. & Meharg, A. The genetics of arsenate tolerance in Yorkshire fog, Holcus lanatus L.. Heredity 69, 325–335 (1992). https://doi.org/10.1038/hdy.1992.132
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DOI: https://doi.org/10.1038/hdy.1992.132
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