Summary
A satellite DNA can be isolated from wheat and barley using Ag+/Cs2SO4 gradients. These DNAs are highly repeated, each with a complexity of about 10 bp. The satellites isolated from the two species cannot be differentiated by physical properties such as buoyant density, melting temperature or renaturation kinetics and heterologous hybrids melt at the same temperature as homologous hybrids. The restriction endonuclease MboII digests both satellites to give identical patterns. These data together with those from digests of RNAs complementary to the separated DNA strands suggest a general formulation of a sequence as (GAA)m (GAG)n. Localisation of the satellite by in situ hybridisation shows it to have major sites on all seven chromosomes of the B genome and chromosomes 4A and 7A in hexaploid wheat, and on all barley chromosomes. There are specific minor sites on other chromosomes of the A and D genomes of wheat.
Similar content being viewed by others
Article PDF
References
Bendich, A J, and McCarthy, B J. 1970. DNA comparisons among barley, oats, rye and wheat. Genetics 65, 545–565.
Bennett, M D, and Smith, J B. 1976. Nuclear DNA amounts in angiosperms. Phil Trans Royal Soc (Lond), B Biological Sciences. 274, 227–274.
Birnstiel, M L, Sells, B H, and Purdom, I F. 1972. Kinetic complexity of RNA molecules. J Mol Biol, 63, 21–39.
Brownlee, G G. 1972. Determination of sequences in RNA, eds. T. S. Work and E. Work. North Holland Publishing Co., Amsterdam.
Brutlag, D L, Carlson, M, Fry, K, and Hsieh, T S. 1977. DNA sequence organization in Drosophila heterochromatin. Cold Spring Harbor Symp Quant Biol, 42, 1137–1146.
Brutlag, D L, and Peacock, W J. 1975. Sequences of highly repeated DNA in Drosophila melanogaster. In: The Eukaryote Chromosome. Aust. Nat. Univ. Press, Canberra.
Burgess, D R, and Jendrisak, J J. 1975. A procedure for the rapid, large scale purification of Escherichia coli DNA-dependent RNA polymerase involving polymin P precipitation and DNA cellulose chromatography. Biochemistry, 14, 4634.
Chapman, V, Miller, T E, and Riley, R. (1976). Equivalence of the A genome of bread wheat with that of Triticum urartu. Genet Res Camb, 27, 69–76.
Endow, S A. 1977. Analysis of Drosophila melanogaster satellite IV with restriction endonuclease Mbo II.. J Mol Biol, 114, 441–449.
Fry, K, and Salser, W. 1977. Nucleotide sequences of HSα satellite from kangaroo rat Dipodomys ordii and characterization of similar sequences in other rodents. Cell, 12, 1069–1084.
Gall, J G, and Atherton, D D. 1974. Satellite DNA sequences in D. virilis. J Mol Biol, 85, 633–664.
Gelinas, R E, Myers, D A, and Roberts, R J. 1977. Two sequence-specific endonucleases from Moraxella bovis. J Mol Biol, 114, 169–179.
Gerlach, W L. 1977. N-banded karyotypes of wheat species. Chromosoma, 62, 49–56.
Gerlach, W L, Appels, R, Dennis, E S, and Peacock, W J. 1978. Evolution and analysis of wheat genomes using highly repeated DNA sequences. Proc 5th Int Wheat Genet Symp, 1, 81–91.
Gerlach, W L, and Peacock, W J. 1979. Chromosomal locations of highly repeated DNA sequences in wheat. Heredity (in press).
Gill, B S, and Kimber, G. 1974. Giemsa C-banding and the evolution of wheat. Proc Nat Acad Sci USA, 71, 4086–4090.
Griffin, B E, and Mirzabekov, A D. 1972. 5S RNA conformation studies of the partial T1 ribonuclease digestion by gel electrophoresis and two dimensional thin layer chromatography. J Mol Biol, 72, 633–643.
Hennig, W, and Walker, P M B. 1970. Variations in the DNA from two rodent families (Cricetiaea and Muridae). Nature, 225, 915–919.
Huguet, T, and Jouanin, L. 1972. Wheat DNA: Study of the heavy satellite in Ag+-Cs2 SO4 density gradient. Biochem Biophys Res Comm, 46, 1169–1174.
Islam, A K M R, Shepherd, K W, and Sparrow, D H B. 1978. Wheat-barley hybrids and addition lines. Proc 5th Int Wheat Genet Symp, 1, 365–371.
Linde-Laursen, I. 1975. Giemsa C-banding of the chromosomes of “Emir” barley. Hereditas, 81, 285–289.
Lohe, A R. 1977. Highly repeated DNA in Drosophila simulons: chromosomal organization and evolutionary stability. Ph.D. Thesis, Australian National University.
Maxam, A M, and Gilbert, W. 1977. A new method for sequencing DNA. Proc Nat Acad Sci USA, 74, 560–564.
McFadden, E S, and Sears, E R. 1946. The origin of Triticum spelta and its free threshing hexaploid derivatives. J Hered, 37, 107–116.
Noda, K, and Kasha, K J. 1977. Barley chromosome identification with the C banding, Giemsa stain technique. Barley Genetics Newsletter 1977, 47–50.
Peacock, W J, Brutlag, D, Goldring, E, Appels, R, Hinton, C N, and Lindsley, D L. 1973. The organization of highly repeated DNA sequences in Drosophila melanogaster chromosomes. Cold Spring Harbor Symp Quant Biol, 38, 405–416.
Peacock, W J, Appels, R, Dunsmuir, P, Lohe, A R, and Gerlach, W L. 1976. Highly, repeated DNA sequences: chromosomal localization and evolutionary conservation. In: International Cell Biology, eds. B. R. Brinkley and K. R. Porter. Rockefeller University Press, pp. 494–506.
Peacock, W J, Lohe, A R, Gerlach, W L, Dunsmuir, P, Dennis, E S, and Appels, R. 1977. Fine structure and evolution of DNA in heterochromatin. Cold Spring Harbor Symp Quant Biol, 42, 1121–1135.
Ranjekar, P K, Palotta, D, and Lafontaine, J G. 1976. Analysis of the genome of plants. II. Characterization of repetitive DNA in barley and wheat. Biochim Biophys Acta, 425, 30–40.
Rigby, P W J, Dieckmann, M, Rhodes, C, and Berg, P. 1976. Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol, 113, 237–251.
Salser, W, Bowens, S, Brown, D, El Adli, F, Fedoroff, N, Fry, K, Heindell, H, Paddock, O, Poon, R, Wallace, B, and Whitcomb, P. 1976. Investigation of the organization of mammalian chromosomes at the DNA sequence level. Fed Proc, 35, 23–35.
Sears, E R. 1963. Chromosome mapping with the aid of telocentrics. Proc. 2nd Int. Wheat Genetics Symp., published in Hereditas, Suppl. Vol. 2, (1966).
Sears, E R. 1966. Nullisomic-tetrasonic combinations in hexaploid wheat. In “Chromosome Manipulations and Plant Genetics”, eds. R. Riley and K. R. Lewis. Oliver and Boyd, Edinburgh, 1966.
Singh, L, Purdom, I F, and Jones, K W. 1977. Effect of different denaturing agents on the detectability of specific DNA sequences of various base composition by in situ hybridization. Chromosoma (Berl), 60, 377–389.
Southern, E M. 1970. Base sequence and evolution of guinea pig a satellite DNA. Nature, 227, 794–798.
Symons, R H. 1974. Synthesis of α32P ribo- and deoxyribonucleoside triphosphates. Methods in Enzymology, 29, 102–115.
Walker, P M B. 1968. How different are the DNAs from related animals. Nature (Lond), 219, 228–232.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Dennis, E., Gerlach, W. & Peacock, W. Identical polypyrimidine-polypurine satellite DNAs in wheat and barley. Heredity 44, 349–366 (1980). https://doi.org/10.1038/hdy.1980.33
Received:
Issue Date:
DOI: https://doi.org/10.1038/hdy.1980.33
This article is cited by
-
Genotyping-by-sequencing and genome-wide association study reveal genetic diversity and loci controlling agronomic traits in triticale
Theoretical and Applied Genetics (2022)
-
Recombination between homoeologous chromosomes induced in durum wheat by the Aegilops speltoides Su1-Ph1 suppressor
Theoretical and Applied Genetics (2019)
-
Molecular cytogenetic analysis reveals evolutionary relationships between polyploid Aegilops species
Plant Systematics and Evolution (2019)
-
Fluorescence in situ hybridization karyotyping reveals the presence of two distinct genomes in the taxon Aegilops tauschii
BMC Genomics (2018)
-
Constructing an alternative wheat karyotype using barley genomic DNA
Journal of Applied Genetics (2015)