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Moderate weight loss depletes intramyocellular triglycerides but has no effect on diglycerides in type II diabetes

Abstract

Intramyocellular diglycerides have been implicated in the development of insulin resistance. We aimed to evaluate the effect of weight loss, a well-known means for the improvement of insulin resistance, on intramyocellular diglycerides and triglycerides in type II diabetes. Participants were five obese women with type II diabetes (body mass index 37.5±1.9 kg/m2), who participated in a weight loss program. The aim of the program was a reduction in body weight by 10%. Muscle biopsies were obtained before and after weight loss. The intervention induced a marked reduction in intramyocellular triglycerides by 77.7±13.1%, whereas no significant differences were observed for diglycerides before and after weight loss. Our results indicate that weight loss due to negative energy balance depletes intramyocellular lipid storage without affecting intermediate molecules of lipid metabolism, such as intramyocellular diglycerides.

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References

  • Anastasiou CA, Kavouras SA, Lentzas Y, Gova A, Sidossis LS, Melidonis A (2009). Diabetes mellitus is associated with increased intramyocellular triglyceride, but not diglyceride, content in obese humans. Metabolism 58, 1636–1642.

    Article  CAS  Google Scholar 

  • Goodpaster BH, Theriault R, Watkins SC, Kelley DE (2000). Intramuscular lipid content is increased in obesity and decreased by weight loss. Metabolism 49, 467–472.

    Article  CAS  Google Scholar 

  • Kelley DE, Goodpaster B, Wing RR, Simoneau JA (1999). Skeletal muscle fatty acid metabolism in association with insulin resistance, obesity, and weight loss. Am J Physiol 277, E1130–E1141.

    Article  CAS  Google Scholar 

  • Matthews DR, Hosker JP, Rudenski AS, Naylor BA, Treacher DF, Turner RC (1985). Homeostasis model assessment: insulin resistance and beta-cell function from fasting plasma glucose and insulin concentrations in man. Diabetologia 28, 412–419.

    Article  CAS  Google Scholar 

  • Nishizuka Y (1995). Protein kinase C and lipid signaling for sustained cellular responses. FASEB J 9, 484–496.

    Article  CAS  Google Scholar 

  • Perseghin G, Scifo P, De Cobelli F, Pagliato E, Battezzati A, Arcelloni C et al. (1999). Intramyocellular triglyceride content is a determinant of in vivo insulin resistance in humans: a 1H-13C nuclear magnetic resonance spectroscopy assessment in offspring of type 2 diabetic parents. Diabetes 48, 1600–1606.

    Article  CAS  Google Scholar 

  • Shetty PS, Henry CJ, Black AE, Prentice AM (1996). Energy requirements of adults: an update on basal metabolic rates (BMRs) and physical activity levels (PALs). Eur J Clin Nutr 50 (Suppl 1), S11–S23.

    PubMed  Google Scholar 

  • Tamura Y, Tanaka Y, Sato F, Choi JB, Watada H, Niwa M et al. (2005). Effects of diet and exercise on muscle and liver intracellular lipid contents and insulin sensitivity in type 2 diabetic patients. J Clin Endocrinol Metab 90, 3191–3196.

    Article  CAS  Google Scholar 

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Acknowledgements

The study was supported by the University Graduate Research Program.

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Correspondence to S A Kavouras.

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Anastasiou, C., Kavouras, S., Lentzas, Y. et al. Moderate weight loss depletes intramyocellular triglycerides but has no effect on diglycerides in type II diabetes. Eur J Clin Nutr 64, 328–330 (2010). https://doi.org/10.1038/ejcn.2009.157

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  • DOI: https://doi.org/10.1038/ejcn.2009.157

Keywords

  • diglycerides
  • insulin resistance
  • intramyocellular lipids
  • obesity
  • skeletal muscle
  • triglycerides

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