Abstract
Background:
Sphingolipids, like phytosphingosine (PS) are part of cellular membranes of yeasts, vegetables and fruits. Addition of PS to the diet decreases serum cholesterol and free fatty acid (FFA) levels in rodents and improves insulin sensitivity.
Objective:
To study the effect of dietary supplementation with PS on cholesterol and glucose metabolism in humans.
Methods:
Twelve men with the metabolic syndrome (MetS) (according to the International Diabetes Federation (IDF) criteria; age 51±2 years (mean±s.e.m.); body mass index (BMI) 32±1 kg/m2) were randomly assigned to 4 weeks of PS (500 mg twice daily) and 4 weeks of placebo (P) in a double-blind cross-over study, with a 4-week wash-out period between both interventions. At the end of each intervention anthropometric measures and serum lipids were measured and an intravenous glucose tolerance test (IVGTT) was performed.
Results:
Phytosphingosine did not affect body weight and fat mass compared with P. PS decreased serum total cholesterol (5.1±0.3 (PS) vs 5.4±0.3 (P) mmol/l; P<0.05) and low-density lipoprotein (LDL)-cholesterol levels (3.1±0.3 (PS) vs 3.4±0.3 (P) mmol/l; P<0.05), whereas it did not alter serum triglyceride and high-density lipoprotein (HDL)-cholesterol levels. In addition, PS lowered fasting plasma glucose levels (6.2±0.3 (PS) vs 6.5±0.3 (P) mmol/l; P<0.05). PS increased the glucose disappearance rate (K-value) by 9.9% during the IVGTT (0.91±0.06 (PS) vs 0.82±0.05 (P) %/min; P<0.05) at similar insulin levels, compared with P, thus implying enhanced insulin sensitivity. PS induced only minor gastrointestinal side effects.
Conclusion:
Dietary supplementation of PS decreases plasma cholesterol levels and enhances insulin sensitivity in men with the MetS.
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References
Alberti KG, Zimmet P, Shaw J (2006). Metabolic syndrome—a new world-wide definition. A consensus statement from the International Diabetes Federation. Diabet Med 23, 469–480.
Barrenetxe J, Aranguren P, Grijalba A, Martinez-Penuela JM, Marzo F, Urdaneta E (2006). Effect of dietary quercetin and sphingomyelin on intestinal nutrient absorption and animal growth. Br J Nutr 95, 455–461.
Berra B, Colombo I, Sottocornola E, Giacosa A (2002). Dietary sphingolipids in colorectal cancer prevention. Eur J Cancer Prev 11, 193–197.
Duivenvoorden I, Voshol PJ, Rensen PC, van Duyvenvoorde W, Romijn JA, Emeis JJ et al. (2006). Dietary sphingolipids lower plasma cholesterol and triacylglycerol and prevent liver steatosis in APOE*3Leiden mice. Am J Clin Nutr 84, 312–321.
Ford ES (2005). Risks for all-cause mortality, cardiovascular disease, and diabetes associated with the metabolic syndrome: a summary of the evidence. Diabetes Care 28, 1769–1778.
Ford ES, Giles WH, Dietz WH (2002). Prevalence of the metabolic syndrome among US adults: findings from the third National Health and Nutrition Examination Survey. JAMA 287, 356–359.
Harder T, Simons K (1997). Caveolae, DIGs, and the dynamics of sphingolipid-cholesterol microdomains. Curr Opin Cell Biol 9, 534–542.
Hendriks HF, Brink EJ, Meijer GW, Princen HM, Ntanios FY (2003). Safety of long-term consumption of plant sterol esters-enriched spread. Eur J Clin Nutr 57, 681–692.
Hendriks HF, Weststrate JA, van Vliet T, Meijer GW (1999). Spreads enriched with three different levels of vegetable oil sterols and the degree of cholesterol lowering in normocholesterolaemic and mildly hypercholesterolaemic subjects. Eur J Clin Nutr 53, 319–327.
Imaizumi K, Tominaga A, Sato M, Sugano M (1992). Effects of dietary sphingolipids on levels of serum and liver lipids in rats. Nutr Res 12, 543–548.
Isomaa B, Almgren P, Tuomi T, Forsen B, Lahti K, Nissen M et al. (2001). Cardiovascular morbidity and mortality associated with the metabolic syndrome. Diabetes Care 24, 683–689.
Lieber CS, Leo MA, Mak KM, Xu Y, Cao Q, Ren C et al. (2004). Acarbose attenuates experimental non-alcoholic steatohepatitis. Biochem Biophys Res Commun 315, 699–703.
Lundback K (1962). Intravenous glucose tolerance as a tool in definition and diagnosis of diabetes mellitus. Br Med J 1, 1507–1513.
Merrill Jr AH, Schmelz EM, Dillehay DL, Spiegel S, Shayman JA, Schroeder JJ et al. (1997). Sphingolipids—the enigmatic lipid class: biochemistry, physiology, and pathophysiology. Toxicol Appl Pharmacol 142, 208–225.
Miettinen TA, Puska P, Gylling H, Vanhanen H, Vartiainen E (1995). Reduction of serum cholesterol with sitostanol-ester margarine in a mildly hypercholesterolemic population. N Engl J Med 333, 1308–1312.
Noh SK, Koo SI (2004). Milk sphingomyelin is more effective than egg sphingomyelin in inhibiting intestinal absorption of cholesterol and fat in rats. J Nutr 134, 2611–2616.
Okada K, Yanagawa T, Warabi E, Yamastu K, Uwayama J, Takeda K et al. (2009). The alpha-glucosidase inhibitor acarbose prevents obesity and simple steatosis in sequestosome 1/A170/p62 deficient mice. Hepatol Res 39, 490–500.
Petersen KF, Dufour S, Befroy D, Lehrke M, Hendler RE, Shulman GI (2005). Reversal of nonalcoholic hepatic steatosis, hepatic insulin resistance, and hyperglycemia by moderate weight reduction in patients with type 2 diabetes. Diabetes 54, 603–608.
Ravikumar B, Gerrard J, Dalla MC, Firbank MJ, Lane A, English PT et al. (2008). Pioglitazone decreases fasting and postprandial endogenous glucose production in proportion to decrease in hepatic triglyceride content. Diabetes 57, 2288–2295.
Schmelz EM, Crall KJ, Larocque R, Dillehay DL, Merrill Jr AH (1994). Uptake and metabolism of sphingolipids in isolated intestinal loops of mice. J Nutr 124, 702–712.
Segal P, Eliahou HE, Petzinna D, Neuser D, Bruckner A, Spengler M (2005). Long-term efficacy and tolerability of acarbose treatment in patients with type 2 diabetes mellitus. Clin Drug Investig 25, 589–595.
Tang JL, Armitage JM, Lancaster T, Silagy CA, Fowler GH, Neil HA (1998). Systematic review of dietary intervention trials to lower blood total cholesterol in free-living subjects. BMJ 316, 1213–1220.
Vesper H, Schmelz EM, Nikolova-Karakashian MN, Dillehay DL, Lynch DV, Merrill Jr AH (1999). Sphingolipids in food and the emerging importance of sphingolipids to nutrition. J Nutr 129, 1239–1250.
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Snel, M., Sleddering, M., Pijl, H. et al. The effect of dietary phytosphingosine on cholesterol levels and insulin sensitivity in subjects with the metabolic syndrome. Eur J Clin Nutr 64, 419–423 (2010). https://doi.org/10.1038/ejcn.2009.154
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DOI: https://doi.org/10.1038/ejcn.2009.154