Evasion of cell death is a hallmark of cancer, and understanding the intrinsic, or mitochondrial, pathway of apoptosis has produced potent targeted BH3-mimetic therapies like venetoclax.
BH3-mimetics activate apoptosis in malignant cells by targeting the B-cell lymphoma 2 (BCL-2) family of proteins that regulate it. However, the BCL-2 family is large with a complex web of interactions that makes predictions of its behaviour challenging.
This webcast will present intracellular BH3 (iBH3), a functional assay optimized for flow cytometry that can advance precision medicine by interrogating the mitochondria directly using a combination of peptides and compounds that specifically target different proteins in the BCL-2 family. iBH3 can detect the commitment to mitochondrial outer membrane permeabilization (MOMP), which correlates with chemosensitivity and can reveal which anti-apoptotic BCL-2 family proteins malignant cells are using for their survival.
Learn how BH3 profiling:
• Can rapidly determine how readily cells will undergo apoptosis
• Can validate the on-target behaviour of new BH3 mimetic drugs
• Reveals the mechanism of resistance to BH3 mimetics
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This webcast has been produced by Sartorius, who retails sole responsibility for content. About this content.
Speaker
Jeremy Ryan, Staff Scientist, Dana-Farber Cancer Institute
Jeremy Ryan earned his M.S. in Chemistry from MIT in 2005. He started in the Letai laboratory as a technician in 2006, with notable contributions including the evolution of BH3 profiling from a demanding ELISA to the modern flow and microscopy platforms. As a staff scientist, he currently leads the BH3 Profiling Flow Core at Dana-Farber where, among other projects, profiling is being adapted to clinical trials as a biomarker in diseases such as CLL, ALL and AML.
Moderator
Nikki Forrester, Freelance Science Writer and Editor
Nikki Forrester is a science journalist who covers biology, natural history, climate, and the culture of academic research. She earned a Ph.D. in ecology and evolutionary biology in 2019.
