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Down-Regulation of TLR9 Expression Affects the Maturation and Function of Murine Bone Marrow-Derived Dendritic Cells Induced by CpG

Cellular & Molecular Immunology volume 6, pages 199–205 (2009)Cite this article

Abstract

Toll-like receptor 9 (TLR9) is expressed intracellularly by dendritic cells (DCs) and specifically recognizes unmethylated CpG motif. Recognition of TLR9 to CpG DNA can induce DC maturation followed by the subsequent immune responses. Here, RNA interference (RNAi) was used to identify the effect of CpG DNA signaling on DC function. The results showed that transfection of DCs with siRNA specific for TLR9 gene significantly down-regulated TLR9 expression. Immature DCs transfected with TLR9 siRNA did not differentiate into mature DCs with exposure to CpG. TLR9 siRNA-treated DCs expressed low levels of MHC II and CD40 without reducing endocytosis. Furthermore, TLR9 siRNA-transfected DCs exhibited a decreased allostimulatory capacity in a lymphocyte proliferation assay and attenuated Th1 responses by decreasing IL-12p70 production. Our findings indicate that siRNA in silencing TLR9 gene in DCs may offer a potential tool to study the TLR9-CpG pathway.

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Authors and Affiliations

  1. Immunology and Reproductive Biology Lab, Medical School & State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, 210093, China

    Ling Ma, Guangfeng Zhao, Chunyan Hua, Xiaoxi Li, Xiaoyin Zhao & Yayi Hou

  2. The affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, 210008, China

    Lingyun Sun

  3. Jiangsu Key Laboratory of Molecular Medicine, Nanjing, 210093, China

    Yayi Hou

Authors
  1. Ling Ma
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  2. Guangfeng Zhao
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  3. Chunyan Hua
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  4. Xiaoxi Li
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  5. Xiaoyin Zhao
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  6. Lingyun Sun
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  7. Yayi Hou
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Correspondence to Lingyun Sun or Yayi Hou.

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Ma, L., Zhao, G., Hua, C. et al. Down-Regulation of TLR9 Expression Affects the Maturation and Function of Murine Bone Marrow-Derived Dendritic Cells Induced by CpG. Cell Mol Immunol 6, 199–205 (2009). https://doi.org/10.1038/cmi.2009.27

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  • DOI: https://doi.org/10.1038/cmi.2009.27

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